A comparison of three decalcification agents for assessments of cranial fracture histomorphology

Cornelison, Jered B.; Isaac, Carolyn; Devota, Clara J; Billian, Joseph; Brown, Theodore; deJong, Joyce L.; Douglas, Elizabeth A; Fisher-Hubbard, Amanda; Lackey‐Cornelison, Wendy; Prahlow, Joseph A.; Shattuck, Brandy

doi:10.1111/1556-4029.14990

Cited by 2 publications

(3 citation statements)

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“…The tibia tissues were fixed in 10% formalin for 24 h, and decalcified with 5% nitric acid solution for eight days (each day the solution was renewed). There are multiple decalcification methods, but nitric acid is a well-balanced option that quickly produces high-quality histological specimens and microscopic slides [ 15 ]. Following the decalcification process, the specimens were taken to the neutralization process with sodium bicarbonate for 3h and then subjected to histological processing.…”

Section: Methodsmentioning

confidence: 99%

Radioprotective role of amifostine on osteointegration of titanium implants in the tibia of rats

et al. 2023

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Background Titanium is the most widely used metal for bone integration, especially for cancer patients receiving ionizing radiation. This study aimed to investigate the amifostine administration that would reduce the effects of radiation on bone healing and osseointegration in rat models. Objectives It is aimed that the application of amifostine in rats receiving radiotherapy treatment will reduce the negative effects of ionizing radiation on the bone. Methods Thirty-five adult male Wistar rats were randomly divided into one healthy and four experimental groups. In three consecutive days, two experimental groups of rats (AMF-RT-IMP and RT-IMP) were exposed to radiation (15 Gy/3 fractions of 5 Gy each). Then the titanium implants were inserted into the left tibia. Before the radiotherapy process, a 200 mg/kg dose of amifostine (AMF) was administered to the rats in the AMF-IMP and AMF-RT-IMP groups. Twenty-eight days after the screw implant, all rats were sacrificed, and their blood samples and tibia bones were collected for analysis. Results The results indicated an accelerated bone formation and a more rapid healing process in the screw implants in the AMF-IMP, AMF-RT-IMP, and AMF-RT groups than in the RT-IMP group. Also, bone-implant contact area measurement and inflammation decreased with amifostine treatment in the implants subjected to irradiation ( p < 0.05). Conclusions The results obtained in the present study suggested that amifostine prevents the losses of bone minerals, bone integrity, and implant position from ionizing-radiation when given before exposure.

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Section: Methodsmentioning

confidence: 99%

Radioprotective role of amifostine on osteointegration of titanium implants in the tibia of rats

et al. 2023

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“…For juvenile or adult bones, however, nitric acid is a better option with faster decalcification that also results in high‐quality histologic slides. For further discussion regarding decalcification agents and their impact on bone histomorphology, please see Cornelison et al [10]. Throughout immersion decalcification, the sample should be monitored radiographically to establish the decalcification endpoint using the setting previously identified for the sample (see Sample Excision and Fixation ).…”

Section: Methodsmentioning

confidence: 99%

“…For juvenile or adult bones, however, nitric acid is a better option with faster decalcification that also results in high-quality histologic slides. For further discussion regarding decalcification agents and their impact on bone histomorphology, please see Cornelison et al[10]. ThroughoutF I G U R E 1 Recommended preprocessing photographic series: (A) fracture in situ, (B) excised fracture sample from an ectocranial view, (C) excised fracture sample from an endocranial view, (D) thick section of the fracture sample from the ectocranial view with forceps indicating the section used for slide development, (E) cross-sectional view of the thick section of the fracture sample, (F) trimmed fracture sample in cross-section.…”

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A method for the development of cranial fracture histology slides

Isaac

Cornelison

Devota

et al. 2022

Journal of Forensic Sciences

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Cranial vault fractures are of medicolegal interest as they have long-term impacts to someone's health and may contribute to an individual's death. The ability to distinguish antemortem from perimortem fractures and to assess the age of the injury is increasingly dependent on histology. Despite the increasing role of histology in assessing the microanatomy of osseous fractures, there are no methods currently available which account for the nuances and difficulties in creating high-quality histologic slides of cranial vault fractures that allow visualization of cellular features associated with healing bone. The authors present a modified method specific to slide development of human cranial vault fractures derived from the trial-and-error process of creating 730 such slides over a 3-year period which are suitable for the evaluation of the tissues, cells, and nuclei involved in fracture healing. This method adapts and troubleshoots typical histological procedures including sample excision, fixation, decalcification, dehydrating, clearing, embedding, microtomy, and staining, and introduces new procedures including preprocessing photography and cassette placement.By implementing these modifications, the number of poor-quality slides that required a new section to be sent to the histology laboratory was greatly reduced. Proactively implementing this new method into cranial fracture histologic slide development significantly reduces the number of slide rejections due to common issues like folding, chatter, or insufficient staining, saving both time and financial resources for forensic practitioners, researchers, and histotechnologists.

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A comparison of three decalcification agents for assessments of cranial fracture histomorphology

Abstract: A n t h r o p o l o g y ; P a t h o l o g y / B i o l o g y

Cited by 2 publications

References 33 publications

Radioprotective role of amifostine on osteointegration of titanium implants in the tibia of rats

Radioprotective role of amifostine on osteointegration of titanium implants in the tibia of rats

A method for the development of cranial fracture histology slides

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