A Comparison of Two Ultrasonic Methods for Detaching Biofilms from Natural Substrata

Mermillod‐Blondin, Florian; Fauvet, Guillaume; Chalamet, A.; Châtelliers, Michel Creuzé des

doi:10.1002/1522-2632(200106)86:3<349::aid-iroh349>3.0.co;2-b

Cited by 30 publications

(10 citation statements)

References 35 publications

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“…However, there is great variability in the literature in terms of these factors, and in any case, our design prevented us from providing precise bacterial counts for comparison. Nevertheless, our findings generally confirm those of Torlak and Sert [ 28 ] and Mermillod-Blondin et al [ 29 ] that the association between a germicidal detergent and ultrasonication is able to reduce the bacterial load on the surfaces analysed.…”

Section: Discussionsupporting

confidence: 92%

Comparative SEM analysis of nine F22 aligner cleaning strategies

et al. 2017

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BackgroundThe orthodontics industry has paid great attention to the aesthetics of orthodontic appliances, seeking to make them as invisible as possible. There are several advantages to clear aligner systems, including aesthetics, comfort, chairside time reduction, and the fact that they can be removed for meals and oral hygiene procedures.MethodsFive patients were each given a series of F22 aligners, each to be worn for 14 days and nights, with the exception of meal and brushing times. Patients were instructed to clean each aligner using a prescribed strategy, and sections of the used aligners were observed under SEM. One grey-scale SEM image was saved per aligner in JPEG format with an 8-bit colour depth, and a total of 45 measurements on the grey scale (“Value” variable) were made. This dataset was analysed statistically via repeated measures ANOVA to determine the effect of each of the nine cleaning strategies in each of the five patients.ResultsA statistically significant difference in the efficacy of the cleaning strategies was detected. Specifically, rinsing with water alone was significantly less efficacious, and a combination of cationic detergent solution and ultrasonication was significantly more efficacious than the other methods (p < 0.05).ConclusionsOf the nine cleaning strategies examined, only that involving 5 min of ultrasonication at 42 k Hz combined with a 0.3% germicidal cationic detergent was observed to be statistically effective at removing the bacterial biofilm from the surface of F22 aligners.

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Section: Discussionsupporting

confidence: 92%

Comparative SEM analysis of nine F22 aligner cleaning strategies

et al. 2017

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“…The differences between grinded and sonicated granules were found to be significant (p<0.05) for all three parameters tested. As was shown earlier by other authors, different types of US applications also may result in considerable differences in detaching biofilms from natural substrates [10]. The inhibition effect of grinding on the PAO activity of biofilm-derived microorganisms could be explained by ammonium fixation by clay.…”

Section: Comparison Of Biofilm Activity In Dependence On Granules Presupporting

confidence: 55%

Abundance and Enzyme Activity of Airborne Microorganisms in the Experimental Green Wall System

Kalniņš¹,

Žorža²,

Sieriņa³

et al. 2020

World Congress on Civil, Structural, and Environmental Engineering

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Recently the modern green wall (GW) systems with active air circulation, higher plants and optimized growth media are becoming increasingly more efficient for indoor air biofiltration. However, the functioning mechanisms of these complex systems are still poorly investigated. This study was focused on the activity of biofilm on the ceramic granules, which has been developed in the experimental GW within four months under realistic office conditions. Microbial abundance on the surface of ceramic granules has been evaluated by the number of culturable heterotrophic bacteria, as well as enzyme activity, i.e., fluorescein diacetate (FDA) hydrolysis and potential ammonium oxidation (PAO). Different pre-treatment types of granules, i.e., grinding and sonication, showed significant (p<0.05) differences in FDA and PAO activities. The microbial activity of biofilm derived from the surface of ceramic granules in pots with Epipremnum aureum placed on the 1 st , 3 rd , 5 th , 7 th and 9 th height levels, did not exhibit a linear dependence on the height. Particularly, the FDA hydrolysis on the granule surface varied in the range from 167.4 µg/g on the 3 rd level up to 463.9 µg/g on the 1 st level. Contribution of the GW to the concentration of airborne microorganisms in the air was found to be negligible. Further experiments will be focused on the GW performance in terms of biodegradation of volatile organic compounds.

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“…These results suggest that the reduction in the number of viable cells in the biofilms was largely due to the removal of biofilm. Several previous studies (Zips et al 1990;Mott et al 1998;Oulahal-Lagsir et al 2000Mermillod-Blondin et al 2001) reported that the successful utilization of ultrasonic apparatus operating at a low frequency (<100 kHz) to dislodge biofilms. As seen from our results, in addition to intensity of acoustic energy and frequency, effectiveness of ultrasound treatment is dependent on the treatment time; extended treatments are generally more effective (Scherba et al 1991).…”

Section: Resultsmentioning

confidence: 99%

Combined effect of benzalkonium chloride and ultrasound againstListeria monocytogenesbiofilm on plastic surface

Torlak

Sert

2013

Lett Appl Microbiol

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Significance and Impact of the Study: Listeria monocytogenes is capable of adhering and forming biofilm on inert surfaces such as plastics. Biofilms are more resistant to disinfectants compared to planktonic cells, and this makes their elimination from food processing facilities a big challenge. Moreover, the emergence of resistant to disinfectants bacteria clearly shows that new biofilm control strategies are required. Among new strategies, the ultrasound treatment attracts attention as a nonchemical and environmentally friendly technology. This study demonstrated that the combination of ultrasound and benzalkonium chloride is a feasible strategy for removal of L. monocytogenes biofilms from plastic food-contact surfaces. AbstractThis study was performed to evaluate the effectiveness of benzalkonium chloride combined with ultrasound in eliminating Listeria monocytogenes biofilm from polystyrene surface. The test strain of L. monocytogenes, previously classified as strong biofilm producer, was grown to form biofilm in tryptic soy broth at 20°C for 6 days in polystyrene specimen containers. The biofilms formed on surface of containers were treated with ultrasound alone, benzalkonium chloride (100 and 400 mg l À1 ) alone and their combination for three different exposure times (1, 5 and 15 min) at room temperature. Sonication was performed using an ultrasound bath at a constant ultrasound frequency of 35 kHz. After treatments, levels of biofilm biomass and viable cells in biofilm were determined using crystal violet staining and XTT assays, respectively. The combined treatment of ultrasound and benzalkonium chloride resulted in significant (P < 0Á05) more decrease in the level of viable cells in the L. monocytogenes biofilm compared to individual treatments of benzalkonium chloride. Our results suggest that the combination of benzalkonium chloride with ultrasound is useful approach for the elimination of L. monocytogenes biofilms from plastic surfaces.

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A Comparison of Two Ultrasonic Methods for Detaching Biofilms from Natural Substrata

Cited by 30 publications

References 35 publications

Comparative SEM analysis of nine F22 aligner cleaning strategies

Comparative SEM analysis of nine F22 aligner cleaning strategies

Abundance and Enzyme Activity of Airborne Microorganisms in the Experimental Green Wall System

Combined effect of benzalkonium chloride and ultrasound againstListeria monocytogenesbiofilm on plastic surface

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