A comparison study between different molecular weight polysaccharides derived fromLentinus edodesand their antioxidant activitiesin vivo

Meng

et al. 2017

CyTA - Journal of Food

Characterization, antioxidant activities and hepatoprotective effects of polysaccharides from prepressing separation apple (Fuji) peel pomace were investigated in this study. Gel permeation chromatography indicated that apple peel polysaccharides (APPs) were a kind of heteropolysaccharides composed of three fractions with homogenous polarity. Both GC-MS and HPLC analysis showed that arabinose, galactose and galacturonic acid were the main monosaccharides in APP. In vitro antioxidant activities of APP were characterized. Furthermore, administration of APP (450 mg/ kg bw) in mice significantly decreased the CCl 4 -induced elevations of serum alanine aminotransferase, asparate aminotransferase and lactic dehydrogenase activities, and hepatic malondialdehyde level and inhibited the decreases in superoxide dismutase and glutathione peroxidase activities caused by CCl 4 . Histopathological observation further confirmed that APP could protect the liver tissues from CCl 4 -induced histological alternation. These findings indicate that APP has the antioxidant and hepatoprotective potentials and provide a scientific basis for comprehensive utilization of pre-pressing separation apple peel pomace.Caracterización, actividad antioxidante y efectos hepatoprotectores de polisacáridos de la separación de la pela de manzana Fuji previamente prensada RESUMEN En este estudio se investigaron la caracterización, la actividad antioxidante y los efectos hepatoprotectores de los polisacáridos de la separación del orujo de pela de manzana (Fuji) previamente prensado. La cromatografía de permeación en gel indicó que los polisacáridos de la pela de manzana (APP) eran un tipo de heteropolisacáridos compuestos de tres fracciones con polaridad homogénea. Los dos análisis GC-MS y HPLC mostraron que la arabinosa, la galactosa y el ácido galacturónico fueron los principales monosacáridos en APP. Se caracterizó la actividad antioxidante in vitro de APP. Además, la administración de APP (450 mg/kg bw) en ratones disminuyó significativamente las elevaciones inducidas con CCl 4 de la alanina aminotransferasa sérica, el aspartato aminotransferasa, la actividad lactato deshidrogenasa y el nivel hepático de malondialdehído, además inhibió la disminución de la actividad de superóxido dismutasa y glutatión peroxidasa causada por CCl 4 . Posteriormente la observación histopatológica confirmó que APP podría proteger los tejidos del hígado de la alternación histológica inducida con Cl 4 . Estos hallazgos indican que APP tiene potencial antioxidante y hepatoprotector, además proporciona una base científica para una utilización integral de la separación de orujo de pela de manzana previamente prensado. ARTICLE HISTORY

Section: Chemical Composition Of Appmentioning

confidence: 98%

Characterization, antioxidant activities and hepatoprotective effects of polysaccharides from pre-pressing separationFujiapple peel

Meng

et al. 2017

CyTA - Journal of Food

Oxidative Medicine and Cellular Longevity

“…Among these, LCB had the highest antioxidant and cytotoxic activity. You et al [30] investigated the protective effects of Lentinus edodes PSs with molecular weights of 25.5, 306.2, and 605.4 kDa on D-galactoseinduced oxidative stress-induced myocardial cells in mice and showed that PSs with medium molecular weight have the strongest protective effect.…”

Section: Reduction Of Cytotoxicity Of Com Crystals Aftermentioning

confidence: 99%

Preprotection of Tea Polysaccharides with Different Molecular Weights Can Reduce the Adhesion between Renal Epithelial Cells and Nano-Calcium Oxalate Crystals

Zhao

Liu

et al. 2020

Crystal adhesion is an important link in the formation of kidney stones. This study investigated and compared the adhesion differences between nano-calcium oxalate monohydrate (COM) and human renal proximal tubule epithelial (HK-2) cells before and after treatment with tea polysaccharides (TPSs) TPS0, TPS1, TPS2, and TPS3 with molecular weights of 10.88, 8.16, 4.82, and 2.31 kDa, respectively. TPS treatment effectively reduced the damage of COM to HK-2 cells, thereby resulting in increased cell activity, decreased release of lactate dehydrogenase, cell morphology recovery, decreased level of reactive oxygen species, increased mitochondrial membrane potential, increased lysosomal integrity, decreased expression of adhesion molecule osteopontin and eversion of phosphatidylserine, and decreased crystal adhesion. Among the TPSs, TPS2 with moderate molecular weight had the best protective effect on cells and the strongest effect on the inhibition of crystal adhesion. Thus, TPS2 may be a potential anticalculus drug.

“…Thus, natural products such as mushrooms containing bioactive compounds can be used to help reduce such damage in the body [55]. As nutraceuticals, they activate endogenous protective system, generation important antioxidant role for the homeostasis of the organism [18] [24], fractions of different molecular weight of polysaccharides [56], crude extract of polysaccharide (LEP) [57] [58] and exudates obtained from the mycelium (DE) [59]. All reports have shown antioxidant activity with high phenolic content ( Table 2).…”

Section: Antioxidant Effects Of L Edodesmentioning

confidence: 99%

A Review on General Nutritional Compounds and Pharmacological Properties of the Lentinula edodes Mushroom

Finimundy¹,

Dillon²,

Henriques³

et al. 2014

FNS

Lentinula edodes is a macrofungus with great potential for therapeutic applications and serves as a model for investigating functional fungi properties and isolating pure compounds for pharmaceutical use. Mushrooms have a great nutritional value and present medicinal molecules including polysaccharides, terpenoids, sterols and lipids, that participate actively in several human disorders and modulate mechanisms involved in the immune system regulation. This review will focus on general nutritional compounds and pharmacological properties of L. edodes.