A complete cleavage map of Neurospora crassa mtDNA obtained with endonucleases Eco RI and Bam HI

Terpstra, Peter; Holtrop, Marijke; Kroon, A.M.

doi:10.1016/0005-2787(77)90318-5

Cited by 27 publications

(12 citation statements)

References 25 publications

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“…The cleavage map of mtDNA obtained with endonucleases Eco RI, Barn HI and Hind III [4] is shown in Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

“…2A and 2B resp.). Hybridization to fragment B1 does not lead to a defined band, due to the large size of B1 (33 000 basepairs) as compared to the mean fragment length of the DNA preparation (15 000 basepairs) [4]. Therefore the majority of DNA fragments to which 24 S rRNA hybridizes, has only one or no Barn H1 cleavage site.…”

Section: Resultsmentioning

confidence: 99%

“…Isolation of mitochondria and mtDNA from Neurospora crassa strain E 5256 has been described [2,4]. Incubation of mtDNA with restriction enzymes and electrophoresis on 0.7% agarose slabgels was done as described previously [4].…”

Section: Methodsmentioning

confidence: 99%

“…Incubation of mtDNA with restriction enzymes and electrophoresis on 0.7% agarose slabgels was done as described previously [4]. Restriction enzymes Eco RI, Hind III and Barn HI were obtained from Miles Laboratories Inc. and from New England Biolabs.…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless it has been possible to construct a complete cleavage map of the mtDNA with restriction enzymes [3,4]. The sum of the fragment lengths is in excellent agreement with the contour length of the intact molecule indicating that the mtDNA is a collection of homogeneous molecules, without major gene repetition [4].…”

Section: Introductionmentioning

confidence: 98%

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The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Terpstra

Holtrop

Kroon

1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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“…The cleavage map of mtDNA obtained with endonucleases Eco RI, Barn HI and Hind III [4] is shown in Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Terpstra

Holtrop

Kroon

1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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Nucleotide sequence and intron structure of the apocytochrome b gene of Neurospora crassa mitochondria.

1983

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The sequence of the apocytochrome b (cob) gene of Neurospora crassa has been determined. The structural gene is interrupted by two intervening sequences of approximately 1260 bp each. The polypeptide encoded by the exons shows extensive homology with the cob proteins of Aspergillus nidulans and Saccharomyces cerevisiae (79% and 60%, respectively). The two introns are, however, located at sites different from those of introns in the cob genes of A. nidulans and S. cerevisiae (which contain highly homologous introns at the same site within the gene). The introns share several short regions of sequence homology (10‐12 bp long) with each other and with other fungal mitochondrial introns. Moreover, the second intron contains a 50 nucleotide long sequence that is highly homologous with sequences within every ribosomal intron of fungal mitochondria sequenced to date. The conserved sequences may allow the formation of a core secondary structure, which is nearly identical in many mitochondrial introns. The conserved secondary structure may be required for intron splicing. The second intron contains an open reading frame, continuous with the preceding exon, of approximately 290 codons. Two stretches of 10 amino acid residues, conserved in many introns, are present in the open reading frame.

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RNA processing in Neurospora crassa mitochondria: use of transfer RNA sequences as signals.

Breitenberger¹,

Browning

Alzner-Deweerd³

et al. 1985

The EMBO Journal

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We have used RNA gel transfer hybridization, S1 nuclease mapping and primer extension to analyze transcripts derived from several genes in Neurospora crassa mitochondria. The transcripts studied include those for cytochrome oxidase subunit III, 17S rRNA and an unidentified open reading frame. In all three cases, initial transcripts are long, include tRNA sequences, and are subsequently processed to generate the mature RNAs. We find that endpoints of the most abundant transcripts generally coincide with those of tRNA sequences. We therefore conclude that tRNA sequences in long transcripts act as primary signals for RNA processing in N. crassa mitochondria. The situation is somewhat analogous to that observed in mammalian mitochondrial systems. The difference, however, is that in mammalian mitochondria, noncoding spacers between tRNA, rRNA and protein genes are very short and in many cases non‐existent, allowing no room for intergenic RNA processing signals whereas, in N. crassa mtDNA, intergenic non‐coding sequences are usually several hundred nucleotides long and contain highly conserved GC‐rich palindromic sequences. Since these GC‐rich palindromic sequences are retained in the processed mature RNAs, we conclude that they do not serve as signals for RNA processing.

show abstract

A complete cleavage map of Neurospora crassa mtDNA obtained with endonucleases Eco RI and Bam HI

Cited by 27 publications

References 25 publications

The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Nucleotide sequence and intron structure of the apocytochrome b gene of Neurospora crassa mitochondria.

RNA processing in Neurospora crassa mitochondria: use of transfer RNA sequences as signals.

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