A complex ensemble of cis-regulatory elements controls the expression of a Vicia faba non-storage seed protein gene

Fiedler, Ulrike; Filistein, Roman; Wobus, Ulrich; Bäumlein, Helmut

doi:10.1007/bf00047407

Cited by 50 publications

(34 citation statements)

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“…The activation potential of ABI3 on P HsfA9 :GUS was abolished by deletion of the RY/Sph motif ( Figure 3C). This observation is consistent with the previous reports where deletion of an RY/Sph motif in the promoter of a legumin gene of V. faba (Bä umlein et al, 1986(Bä umlein et al, , 1991a(Bä umlein et al, , 1991bFiedler et al, 1993), the promoter of a napin gene of Brassica napus (Ellerströ m et al, 1996), and the C1 promoter of Z. mays (Suzuki et al, 1997) abolished most of the seed-specific promoter activity. Interestingly, FUS3 was inactive on P HsfA9 :GUS ( Figure 3B); however, both B3 domain transcription factors (ABI3 and FUS3) have been shown to recognize the same RY/Sph core motif (Reidt et al, 2000;Mö nke et al, 2004).…”

Section: Hsfa9 Is Specifically Expressed In Seeds and Regulated By Abi3supporting

confidence: 82%

A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development ofArabidopsis

et al. 2007

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Within the Arabidopsis thaliana family of 21 heat stress transcription factors (Hsfs), HsfA9 is exclusively expressed in late stages of seed development. Here, we present evidence that developmental expression of HsfA9 is regulated by the seed-specific transcription factor ABSCISIC ACID–INSENSITIVE3 (ABI3). Intriguingly, ABI3 knockout lines lack detectable levels of HsfA9 transcript and protein, and further ectopic expression of ABI3 conferred the ability to accumulate HsfA9 in response to abscisic acid in transgenic plantlets. Consequently, the most abundant heat stress proteins (Hsps) in seeds (Hsp17.4-CI, Hsp17.7-CII, and Hsp101) were not detectable in the ABI3 knockout lines, but their expression could be detected in plants ectopically expressing HsfA9 in vegetative tissues. Furthermore, this seed-specific transcription factor cascade was reconstructed in transient β-glucuronidase reporter assays in mesophyll protoplasts by showing that ABI3 could activate the HsfA9 promoter, whereas HsfA9 in turn was shown to be a potent activator on the promoters of Hsp genes. Thus, our study establishes a genetic framework in which HsfA9 operates as a specialized Hsf for the developmental expression of Hsp genes during seed maturation.

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Section: Hsfa9 Is Specifically Expressed In Seeds and Regulated By Abi3supporting

confidence: 82%

A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development ofArabidopsis

et al. 2007

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“…Progressive 5 ~ deletions in the Psl promoter resulted in gradual decrease of the expression in seed. In contrast to some other studies [3,6,21,25,37,42], negative elements were not found with the deletions shown in Fig. 1.…”

Section: Discussioncontrasting

confidence: 54%

“…So-called RY repeats were found to play an important role in several seed-specific promoters. In some promoters the RY repeat has been reported to function as a positive element [1,7,11,20] but in another case as a negative element [6]. A sequence with homology to the RY repeat is also present in the Psl promoter (located at -16 to -1), but whether it is functional is unknown.…”

Section: Discussionmentioning

confidence: 99%

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The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds

et al. 1996

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The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit at a low level. This fragment contains a 22 bp element (W1), which has been demonstrated to be important for seed-specific expression when coupled as a trimer to a heterologous TATA box (de Pater et al., Plant Cell 5:877-886, 1993). Here we show that deletion of W1 in the natural promoter context resulted in a strongly decreased level of gene expression. A 4 bp mutation of W1 reduced the expression of truncated derivatives of the Psl promoter. A single copy of W1 coupled to the TATA box of the CaMV 35S promoter directed low gene expression in seeds and leaves. Multimerization enhanced the expression in seeds up to 100-fold, to levels found with the Psl promoter, whereas the expression level in leaves remained low. These results demonstrate that the W1 element is an essential control element in the Psl promoter. When taken out of its natural context and multimerized, it is sufficient for high expression in seeds.

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“…The RY cis-motif is required to maintain seed-specific promoter activity (Reidt et al, 2000) and acts as a key regulator during late embryogenesis. This motif is widely distributed in seed-specific gene promoters of dicots and monocots, such as the USP gene (Fiedler et al, 1993) and the napin gene (Ellerström et al, 1996). The RY motif is considered to play an alternative role as a negative element repressing expression in non-seed tissues (Morton et al, 1995).…”

Section: Structure Of the 5' Promoter Region Of Cumft1mentioning

confidence: 99%

Isolation and Characterization of a Citrus FT/TFL1 Homologue (CuMFT1), Which Shows Quantitatively Preferential Expression in Citrus Seeds

Nishikawa

Endo

Shimada

et al. 2008

J. Japan. Soc. Hort. Sci.

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A Citrus FT/TFL1 homologue (CuMFT1) was isolated from Satsuma mandarin (Citrus unshiu Marc.), and a high degree of expression was detected in mature seeds, indicating that its promoter is expected to induce the expression of a target gene in the late embryonic stage of Citrus seed. To obtain a seed-specific promoter useful for Citrus transgenic research, the 5' upstream region of CuMFT1 was isolated from the BAC library, and its promoter activity was characterized using particle bombardment and transgenic Arabidopsis. The 2.4 kbp in the 5' upstream region (CuMFT1 promoter) contained RY (CATGCAT), E-box (CANNTG), and distant B-box (GCCACTTGTC) cis-elements, which have been reported to promote seed-specific gene expression in plants. The CuMFT1 promoter fused to the uidA gene was directly incorporated into tissues from Citrus and its relatives using particle bombardment. The results showed that the CuMFT1 promoter conferred high β-glucronidase (GUS) activity in seed. The CuMFT1 promoter-GUS fusion construct was also incorporated into Arabidopsis, and transgenic Arabidopsis was evaluated by histochemical staining and fluorometric GUS analysis. The experiments revealed that the CuMFT1 promoter conferred quantitatively preferential expression in Arabidopsis seeds; thus, it was suggested that the cis-elements in the CuMFT1 promoter required for expression in Citrus seed were functionally conserved in the heterologous Arabidopsis plant. The CuMFT1 promoter could be utilized as a promoter regulating the quantitatively preferential expression in seed, and is useful for studies of seed development and manipulation by genetic engineering in Citrus and Arabidopsis.

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A complex ensemble of cis-regulatory elements controls the expression of a Vicia faba non-storage seed protein gene

Cited by 50 publications

References 34 publications

A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development ofArabidopsis

A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development ofArabidopsis

The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds

Isolation and Characterization of a Citrus FT/TFL1 Homologue (CuMFT1), Which Shows Quantitatively Preferential Expression in Citrus Seeds

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