A component of green tea, (−)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins

Qin, Jie; Xie, Liping; Zheng, Xiangyi; Wang, Yunbin; Bai, Yu; Shen, Huafeng; Li, Long-Cheng; Dahiya, Rajvir

doi:10.1016/j.bbrc.2007.01.003

Cited by 129 publications

(85 citation statements)

References 26 publications

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“…These catechins have demonstrated great potential as anti-carcinogenic agents (Wang et al, 1992;Qin et al, 2007), presumably via anti-oxidant and cell signaling properties. Our results revealed that PP-60, ECG and EGCG differentially protect human bladder cells against H 2 O 2 -induced stress/apoptosis mediated partially via superoxide and/or direct H 2 O 2 mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effects of green tea and its polyphenols on bladder cells

et al. 2008

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Genitourinary tract inflammation/ailments affect the quality of life and health of a large segment of society. In recent years, studies have demonstrated strong anti-oxidant effects of green tea and its associated polyphenols in inflammatory states. This in vitro study examined the antioxidant capabilities (and putative mechanisms of action) of green tea extract (GTE), polyphenon-60 (PP-60, 60 % pure polyphenols), (−)-epicatechin-3-gallate (ECG) and (−)-epigallocatechin-3-gallate (EGCG) in normal/malignant human bladder cells following catechin treatment ± 1 mM H 2 O 2 (oxidative agent). Cell viability, apoptosis and reactive oxygen species (ROS) formation were evaluated. Our results showed that H 2 O 2 exposure significantly reduced normal (UROtsa) and highgrade (TCCSUP, T24) bladder cancer (BlCa) cell viability compared with control-treated cells (p < 0.001). No affect on low-grade RT4 and SW780 BlCa cell viability was observed with exposure to H 2 O 2 . Compared to H 2 O 2 -treated UROtsa, treatment with PP-60, ECG and EGCG in the presence of H 2 O 2 significantly improved UROtsa viability (p < 0.01), with strongest effects evoked by ECG. Additionally, though not as effective as in UROtsa cells, viability of both high-grade TCCSUP and T24 BlCa cells, in comparison to H 2 O 2 -treated cells, were significantly improved (p < 0.01) by treatment with PP-60, ECG, and EGCG in the presence of H 2 O 2 . Overall, our findings demonstrate that urothelium cell death via H 2 O 2 -induced oxidative stress is mediated, in part, through superoxide (O 2 −· ), and potentially, direct H 2 O 2 mechanisms, suggesting that green tea polyphenols can protect against oxidative stress/damage and bladder cell death.

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Section: Discussionmentioning

confidence: 99%

Antioxidant effects of green tea and its polyphenols on bladder cells

et al. 2008

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“…17 Another example is (-)-epigallocatechin gallate, a phytochemical in green tea, that has been seen to reduce T24 UCC cell proliferation and viability in a dose-and time-dependent manner thought because of inactivation of the PI3K/Akt pathway. 77,78 Perhaps, some of the more popular alternatives to mTORspecific inhibition have been in targeting PI3K and Akt. Although clinical studies evaluating the role of PI3K inhibition PI3K inhibition is comprised of both pan-PI3K inhibitors and isoform-specific inhibitors.…”

Section: Future Directions-other Therapiesmentioning

confidence: 99%

Expanding therapeutic targets in bladder cancer: the PI3K/Akt/mTOR pathway

Ching

Hansel

2010

Laboratory Investigation

139

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“…Many studies conducted on cell-culture systems and animal models as well as human epidemiological studies show that tea could afford protection against a variety of cancer types (Qin et al, 2007;Thakur et al, 2012;Zhong et al, 2012;He et al, 2013;Henning et al, 2013). Several laboratory studies have tried to investigate the link between tea and renal cell carcinoma (Yoshioka et al, 1999;Gu et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Well-established risk factors for renal cell carcinoma include smoking (Asal et al, 1988;Mellemgaard et al, 1994;McLaughlin et al, 1995;Yuan et al, 1998;Bulgheroni et al, 2000;Theis et al, 2008), high intakeZheng-Hui Hu, Yi-Wei Lin, Xin Xu, Hong Chen, Ye-Qing Mao, Jian Wu, Xiang-Lai Xu, Yi Zhu, Shi-Qi Li, Xiang-Yi Zheng, Li-Ping Xie* cell proliferation and tumour-promotion related events (Carlson et al, 2007;Qin et al, 2007;Chen and Dou, 2008;Yang et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

No Association Between Tea Consumption and Risk of Renal Cell Carcinoma: A Meta-analysis of Epidemiological Studies

Hu¹,

Lin²,

Xu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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A component of green tea, (−)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins

Cited by 129 publications

References 26 publications

Antioxidant effects of green tea and its polyphenols on bladder cells

Antioxidant effects of green tea and its polyphenols on bladder cells

Expanding therapeutic targets in bladder cancer: the PI3K/Akt/mTOR pathway

No Association Between Tea Consumption and Risk of Renal Cell Carcinoma: A Meta-analysis of Epidemiological Studies

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