A comprehensive mRNA expression analysis of developing chicken articular cartilage

Singh, Pratik; Ray, Ayan; Azad, Kimi; Bandyopadhyay, Amitabha

doi:10.1016/j.gep.2015.11.001

Cited by 15 publications

(14 citation statements)

References 41 publications

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“…To characterize molecular changes in chick knee joints upon immobilization, we selected 10 genes of interest by comparing our previous transcriptomic analysis of genes specifically expressed in the presumptive chick articular cartilage (Singh et al, 2016) with differentially expressed genes in wild-type and Sp d muscle-less mouse humeri (Rolfe et al, 2014b) (Table S1A). All genes showed altered patterns of expression in immobilized chick knee joints that agree with the altered expression (up or downregulated) found in RNAseq analysis of muscle-less mouse embryos (Table S1A).…”

Section: Molecular Changes At the Joint In Immobilized Chick Embryos mentioning

confidence: 99%

“…RNA in situ hybridization cDNA clones used to make digoxigenin-labeled antisense riboprobes generated by in vitro transcription are detailed in Table S1. RNA in situ hybridization was performed as described previously for chick (Singh et al, 2016) and mouse (Nowlan et al, 2008). Number of specimens analyzed for each gene (n) is given in Table S1.…”

Section: Tissue Processingmentioning

confidence: 99%

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Precise spatial restriction of BMP signaling in developing joints is perturbed upon loss of embryo movement

et al. 2018

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Dynamic mechanical loading of synovial joints is necessary for normal joint development, as evidenced in certain clinical conditions, congenital disorders and animal models where dynamic muscle contractions are reduced or absent. Although the importance of mechanical forces on joint development is unequivocal, little is known about the molecular mechanisms involved. Here, using chick and mouse embryos, we observed that molecular changes in expression of multiple genes analyzed in the absence of mechanical stimulation are consistent across species. Our results suggest that abnormal joint development in immobilized embryos involves inappropriate regulation of Wnt and BMP signaling during definition of the emerging joint territories, i.e. reduced β-catenin activation and concomitant upregulation of pSMAD1/5/8 signaling. Moreover, dynamic mechanical loading of the developing knee joint activates Smurf1 expression; our data suggest that Smurf1 insulates the joint region from pSMAD1/5/8 signaling and is essential for maintenance of joint progenitor cell fate.

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Section: Molecular Changes At the Joint In Immobilized Chick Embryos mentioning

confidence: 99%

Section: Tissue Processingmentioning

confidence: 99%

Precise spatial restriction of BMP signaling in developing joints is perturbed upon loss of embryo movement

et al. 2018

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“…In mammals, Irx1 and Irx2 are expressed in the developing interphalangeal joints, and either mouse IRX1 or zebrafish Irx7 can repress the chondrogenic differentiation of a murine chondrogenic cell line . In addition to Iroquois genes, the homolog of the Tricho‐rhino‐Phalangeal syndrome gene TRPS1 is expressed at joints in zebrafish, as well as in early chondrocytes of the murine growth plate . Loss of Trps1 results in precocious chondrocyte maturation in the condylar cartilage of the murine jaw joint, and cone‐shaped epiphyses, joint hypermobility, and osteoarthritis‐like changes to articular cartilage in humans .…”

Section: Controlling Chondrocyte Identity At the Jointmentioning

confidence: 99%

Building and maintaining joints by exquisite local control of cell fate

Smeeton

Askary

Crump

2016

WIREs Developmental Biology

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We owe the flexibility of our bodies to sophisticated articulations between bones. Establishment of these joints requires the integration of multiple tissue types: permanent cartilage that cushions the articulating bones, synovial membranes that enclose a lubricating fluid-filled cavity, and a fibrous capsule and ligaments that provide structural support. Positioning the prospective joint region involves establishment of an “interzone” region of joint progenitor cells within a nascent cartilage condensation, which is achieved through the interplay of activators and inhibitors of multiple developmental signaling pathways. Within the interzone, tight regulation of BMP and TGFβ signaling prevents the hypertrophic maturation of joint chondrocytes, in part through downstream transcriptional repressors and epigenetic modulators. Synovial cells then acquire further specializations through expression of genes that promote lubrication, as well as the formation of complex structures such as cavities and entheses. Whereas genetic investigations in mice and humans have uncovered a number of regulators of joint development and homeostasis, recent work in zebrafish offers a complementary reductionist approach toward understanding joint positioning and the regulation of chondrocyte fate at joints. The complexity of building and maintaining joints may help explain why there are still few treatments for osteoarthritis, one of the most common diseases in the human population. A major challenge will be to understand how developmental abnormalities in joint structure, as well as postnatal roles for developmental genes in joint homeostasis, contribute to birth defects and degenerative diseases of joints.

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“…We next analysed the interaction between the pelvic anlagen and the lateral tissues: the femoral anlagen and the interzone, a mesenchymal tissue present in the joint region between the articulating skeletal elements only during the morphogenetic stage, which secretes several signalling molecules [ 23 , 24 ]. We isolated the femoral anlagen, to the proximal end of which the hip interzone is attached (electronic supplementary material, figure S1a,b), and placed it in contact with the isolated pelvic anlagen, in an area where no perforation occurs during normal development ( figure 3 a ).…”

Section: Resultsmentioning

confidence: 99%

Morphogenetic mechanism of the acquisition of the dinosaur-type acetabulum

et al. 2018

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Understanding morphological evolution in dinosaurs from a mechanistic viewpoint requires the elucidation of the morphogenesis that gave rise to derived dinosaurian traits, such as the perforated acetabulum. In the current study, we used embryos of extant animals with ancestral- and dinosaur-type acetabula, namely, geckos and turtles (with unperforated acetabulum), and birds (with perforated acetabulum). We performed comparative and experimental analyses, focusing on inter-tissue interaction during embryogenesis, and found that the avian perforated acetabulum develops via a secondary loss of cartilaginous tissue in the acetabular region. This cartilage loss might be mediated by inter-tissue interaction with the hip interzone, a mesenchymal tissue that exists in the embryonic joint structure. Furthermore, the data indicate that avian pelvic anlagen is more susceptible to paracrine molecules, e.g. Wnt ligand, secreted by the hip interzone than ‘reptilian’ anlagen. We hypothesize that during the emergence of dinosaurs, the pelvic anlagen became susceptible to the Wnt ligand, which led to the loss of the cartilaginous tissue and to the perforation in the acetabular region. Thus, the current evolutionary-developmental biology study deepens our understanding of morphological evolution in dinosaurs and provides it with a novel perspective.

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A comprehensive mRNA expression analysis of developing chicken articular cartilage

Cited by 15 publications

References 41 publications

Precise spatial restriction of BMP signaling in developing joints is perturbed upon loss of embryo movement

Precise spatial restriction of BMP signaling in developing joints is perturbed upon loss of embryo movement

Building and maintaining joints by exquisite local control of cell fate

Morphogenetic mechanism of the acquisition of the dinosaur-type acetabulum

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