A comprehensive review on the phytochemical and pharmacological aspects of Podophyllum hexandrum: a high value medicinal plant

Rather, Muzafar Ahmad

doi:10.19046/abp.v03i04.06

Cited by 11 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that the process of cancer development is closely linked to the biochemical processes of cell cycle and apoptosis. Currently, the drug discovery process focuses on chemotherapeutic agents which can target the cell cycle and apoptosis [9][10][11][12][13][14].…”

Section: Cells With Decreased λψMmentioning

confidence: 99%

Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential

Long¹,

Huang²,

Yu³

et al. 2019

aoms

View full text Add to dashboard Cite

A b s t r a c t Introduction:The purpose of the present study was to evaluate the antiproliferative activity of dehydrocostus lactone against human BON-1 cancer cell lines and to explore the possible underlying mechanism. Material and methods: MTT cell viability assay was used to determine cytotoxic effects of dehydrocostus lactone in BON-1 cells. Fluorescence and transmission electron microscopic (TEM) techniques were used to study the effect of the compound on cellular morphology and apoptosis. Flow cytometry was used to assess the effect on cell cycle phase distribution. Effects of the drug on cell apoptosis and mitochondrial membrane potential were analyzed by flow cytometry using annexin v and rhodamine-123 as fluorescent probes. Results: The results of the present study indicated that dehydrocostus lactone significantly (p < 0.01) inhibited the growth of BON-1 cancer cells. These growth inhibitory effects of dehydrocostus lactone on BON-1 were found to be time and concentration-dependent. The IC50 of dehydrocostus lactone were found to be 71.9 μM and 52.3 μM at 24 and 48 h time intervals respectively. The growth inhibitory effects of dehydrocostus lactone were found to be due to loss of mitochondrial membrane potential, the induction of apoptosis and sub-G1 cell cycle arrest. Conclusions: Dehydrocostus inhibits in vitro gastrinoma cancer cell growth and therefore may prove beneficial in the management of gastrinoma cancer.

show abstract

Section: Cells With Decreased λψMmentioning

confidence: 99%

Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential

Long¹,

Huang²,

Yu³

et al. 2019

aoms

View full text Add to dashboard Cite

show abstract

“…Methylwogonin also inhibited cancer cell invasion in a dose-dependent manner. Previous studies have reported that the activation of the PI3K/AKT/mTOR pathway plays a vital role in ovarian cancer tumorigenesis, progression and chemotherapy resistance [27, 28]. Therefore we evaluated the effect of methylwogonin on this pathway.…”

Section: Discussionmentioning

confidence: 99%

Methylwogonin exerts anticancer effects in A375 human malignant melanoma cells through apoptosis induction, DNA damage, cell invasion inhibition and downregulation of the mTOR/PI3K/Akt signalling pathway

Chen

Huang

Liu

et al. 2019

aoms

View full text Add to dashboard Cite

Introduction The main purpose of the present research was to study the anticancer effects of methylwogonin in A375 human malignant melanoma cells by evaluating its effects on apoptosis, DNA fragmentation, cancer cell invasion and the mTOR/PI3K/AKT signalling pathway. Material and methods Effects on cell cytotoxicity were evaluated by MTT assay while a clonogenic assay determined the effects of methylwogonin on colony formation. Fluorescence microscopy evaluated apoptotic effects of methylwogonin in these cells using acridine orange/propidium iodide and Hoechst 33342 staining dyes. Gel electrophoresis evaluated the effects of methylwogonin on DNA fragmentation while the Matrigel invasion assay evaluated the effects of the drug on cancer cell invasion. Effects of methylwogonin on the mTOR/PI3K/AKT signalling pathway were evaluated by western blot assay. Results Methylwogonin induces concentration-dependent as well as time-dependent growth inhibitory effects inducing significant cytotoxicity in these cancer cells. Methylwogonin led to dose-dependent inhibition of colony formation in A375 human malignant melanoma cells. The number of cell colonies decreased significantly as the methylwogonin dose increased from 0, 50, 150, to 300 μM. Methylwogonin treatment of cells at lower doses led to yellow fluorescence (early apoptosis), which changed to red/orange fluorescence, indicating late apoptosis at higher doses. Similar results were obtained using Hoechst 33342 staining, revealing that 50, 150 and 300 μM doses of methylwogonin led to significant morphological changes including chromatin condensation, fragmented nuclei and cellular shrinkage. DNA ladder formation was also observed, and this effect increased with increasing doses of methylwogonin. Methylwogonin also inhibited cancer cell invasion in a dose-dependent manner. Conclusions Different doses of methylwogonin led to concentration-dependent downregulation of phosphorylated PI3K, AKT and mTOR.

show abstract

“…The genus Podophyllum is generally represented by three species including Podophyllum hexandrum, Podophyllum peltatum, and Podophyllum sikkimensis. Of these Podophyllum species, Podophyllum hexandrum Royle is medicinally more valuable, containing comparatively higher concentration of podophyllotoxin (Muzafar and Shajrul, 2016) [35] . P. hexandrum is a source of several physiologically significant metabolites with cytotoxic, antifungal, anti-inflammatory, antioxidant, and radioprotective properties (Prakash et al, 2005) [36] .…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, traditional uses and pharmacological potential of Podophyllum hexandrum: An endangered medicinal plant of North-Western Himalayas

Altaf,

Mushtaq,

Sofi

et al. 2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

Podophyllum hexandrum Royle is a useful medicinal plant and is found in the lower Himalayan zone at elevations between 2000 and 4500 meters. This herbaceous perennial plant has a number of secondary metabolites that are significant from a pharmacological standpoint. The most significant of them is podophyllotoxin, which is utilized to treat specific types of cancer and has cytotoxic and antitumor effects. Due to its vast medicinal potential, plants are also valued in many traditional medical systems. The frequency of this species has decreased in recent years due to overexploitation of the plant. The botanical, medicinal, phytochemical and pharmacological features of the plant are briefly reviewed in this article.

show abstract

A comprehensive review on the phytochemical and pharmacological aspects of Podophyllum hexandrum: a high value medicinal plant

Cited by 11 publications

References 52 publications

Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential

Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential

Methylwogonin exerts anticancer effects in A375 human malignant melanoma cells through apoptosis induction, DNA damage, cell invasion inhibition and downregulation of the mTOR/PI3K/Akt signalling pathway

Chemical composition, traditional uses and pharmacological potential of Podophyllum hexandrum: An endangered medicinal plant of North-Western Himalayas

Contact Info

Product

Resources

About