Dehydrocostus lactone inhibits in vitro gastrinoma cancer cell growth through apoptosis induction, sub-G1 cell cycle arrest, DNA damage and loss of mitochondrial membrane potential

Abstract

A b s t r a c t Introduction:The purpose of the present study was to evaluate the antiproliferative activity of dehydrocostus lactone against human BON-1 cancer cell lines and to explore the possible underlying mechanism. Material and methods: MTT cell viability assay was used to determine cytotoxic effects of dehydrocostus lactone in BON-1 cells. Fluorescence and transmission electron microscopic (TEM) techniques were used to study the effect of the compound on cellular morphology and apoptosis. Flow cytometry was used to assess the effect on cell cycle phase distribution. Effects of the drug on cell apoptosis and mitochondrial membrane potential were analyzed by flow cytometry using annexin v and rhodamine-123 as fluorescent probes. Results: The results of the present study indicated that dehydrocostus lactone significantly (p < 0.01) inhibited the growth of BON-1 cancer cells. These growth inhibitory effects of dehydrocostus lactone on BON-1 were found to be time and concentration-dependent. The IC50 of dehydrocostus lactone were found to be 71.9 μM and 52.3 μM at 24 and 48 h time intervals respectively. The growth inhibitory effects of dehydrocostus lactone were found to be due to loss of mitochondrial membrane potential, the induction of apoptosis and sub-G1 cell cycle arrest. Conclusions: Dehydrocostus inhibits in vitro gastrinoma cancer cell growth and therefore may prove beneficial in the management of gastrinoma cancer.

Antioxidative, antiproliferative, and apoptosis effect of Coleus tuberosus flesh and peel ethanol extracts on cervical cancer cell lines

“…Increased concentrations of the CPEE impact cell death, as evidenced by an increase in the percentage of sub-G1 (M1) cells and an increase in cell cycle arrest G0-G1 phase. This finding is consistent with previous research indicating that increasing the concentration of extracts of a cell-induced product increases cell death by increasing sub-G1 (M) and G0-G1 cell cycle arrest [56,57]. The CPEE could inhibit the cell cycle, causing cell accumulation in a hyperdiploid, and then undergo apoptosis.…”

Mechanism of Action of Novel Pyrazole Carboxamide Containing a Diarylamine Scaffold against Rhizoctonia solani

“…Fluorescent dye rhodamine 123 can directly distribute in the mitochondrial matrix according to the intensity of the MMP, and its fluorescence intensity can reflect the change of the MMP . Therefore, rhodamine 123 could be used as an indicator to detect the effect of compound SCU2028 on the MMP of R.…”

“…Fluorescent dye rhodamine 123 can directly distribute in the mitochondrial matrix according to the intensity of the MMP, and its fluorescence intensity can reflect the change of the MMP. 23 Therefore, rhodamine 123 could be used as an indicator to detect the effect of compound SCU2028 on the MMP of R. solani. As shown in Figure 3, the result showed that the fluorescence intensity of the mycelium mitochondria in the control group was strong, which indicated that the mitochondrial membrane was complete (Figure 3A).…”

Chemical Composition, Antioxidant and Anticancer Activities of Leptocarpha rivularis DC Flower Extracts

“…Research has associated the anticancer activities of dehydrocostus lactone to the inhibition of cancer cell proliferation and induction of cancer cell apoptosis [ 37 ], inhibition of migration and invasion and inhibition of angiogenesis [ 38 ]. The induction of apoptosis is associated with increased protein [ 37 ], a reduction in mitochondrial membrane potential [ 39 ] and the release of cytochrome C, triggering the intrinsic pathway of apoptosis and/or the activation of caspases [ 40 , 41 , 42 ]. Another, caryophyllene oxide, possesses strong anticancer properties [ 43 , 44 ].…”