A conceptual air-lift reactor design for large scale animal cell cultivation in the context of in vitro meat production

Li, Xueliang; Zhang, Guoqiang; Zhao, Xinrui; Zhou, Jingwen; Du, Guocheng; Chen, Jian

doi:10.1016/j.ces.2019.115269

Cited by 75 publications

(44 citation statements)

References 55 publications

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“…Both in WoS (in Topic, as of December 31, 2019) and Factiva (as of December 31, 2019) databases, we searched for articles containing the following words: "artificial meat" OR "meat in vitro" OR "in vitro meat" OR "cultured meat" OR "synthetic meat" OR "lab-grown meat" OR "lab meat" OR "cell-based meat" OR "clean meat" OR "fake meat" OR "slaughter-free meat" OR "cell-cultured meat" OR "craft meat" OR "cultivated meat" OR "victimless meat" OR "animal-free meat" OR "cruelty-free meat" OR "shmeat" OR "Frankenmeat" OR "test Cultured meat (97) a Edelman et al, 2005;Bhat and Fayaz, 2011;Forgacs et al, 2012;Post, 2012;Hopkins, 2015;Bryant and Barnett, 2018;Hamdan et al, 2018;Bodiou et al, 2020;Chriki and Hocquette, 2020;Weinrich et al, 2020;Zhang et al, 2020 in vitro meat (85) Datar and Betti, 2010;Laestadius, 2015;Sharma et al, 2015;Hocquette, 2016;Wilks and Phillips, 2017;Lee, 2018;Bhat et al, 2019;Bryant and Barnett, 2019;Woll, 2019;Li et al, 2020 Clean meat (25) Lagally and Specht, 2017;Windhorst, 2018Windhorst, , 2019 Artificial meat (21) Bonny et al, 2015Bonny et al, , 2017Hocquette, 2015;Hocquette et al, 2015;Orzechowski, 2015;Sodhi, 2017 Synthetic meat (19) Kadim et al, 2015;Marcu et al, 2015;Jones, 2017;Siegrist and Sütterlin, 2017;Lynch and Pierrehumbert, 2019;Warner, 2019 Cell-based meat (10)/cell-cultured meat (1)/cellular meat (1) <...>…”

Section: Abstractelectionmentioning

confidence: 99%

Analysis of Scientific and Press Articles Related to Cultured Meat for a Better Understanding of Its Perception

et al. 2020

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Cultured meat is presented by its advocates as a good alternative for consumers who want to be more ethically minded but who do not wish to change their diet. This novel food has become an emerging topic in both the scientific field and the press media. From a bibliometric analysis of scientific publications and on a sociometric analysis of the mainstream press, the aim of this study was to identify potential differences between the scientific view and the public perception. This research analyzed the publications indexed by SCI-EXPANDED in the Web of Science Core Collection database owned by Clarivate Analytics, for scientific literature analysis, and indexed by the Factiva database, for the press media. A total of 327 scientific publications were analyzed according to year of publication and country and institution of origin, also including coauthorships, co-citations, and scientific fields' and journals' networks. A knowledge mapping using VOSviewer was used to study the literature in the field. Based on Factiva, 12,900 press articles dealing with artificial meat, mainly in English, have been found through public databases. The main conclusion is that cultured meat is mainly developing in the USA and the UK, with other countries, such as China, observing the trend for potential future applications. Scientific articles seemed initially to focus mainly on technical aspects of artificial meat and more recently on health value, consumer's acceptance, and sustainability. However, the potential environment-friendly effects of this novel food are more and more studied or described in scientific or press articles.

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Section: Abstractelectionmentioning

confidence: 99%

Analysis of Scientific and Press Articles Related to Cultured Meat for a Better Understanding of Its Perception

et al. 2020

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“…However, the impeller-mediated mixing can induce very high shear stresses on the cells [ 65 ], which is why many CM startups turn to rocking platform BRs, that causes lower shear stress, due to gentle wave-like fluid motion in the cellbag [ 66 ]. Other promising candidates include hollow fiber [ 8 ] and air-lift [ 67 ] bioreactors, but also modifications of the conventional stirred tank and rocking platform BRs, such as by other methods for inducing the wave-motion, e.g., by using a horizontal displacement in combination with a rocking motion, which can increase the mass transfer capacity [ 68 ].…”

Section: Bioreactors and Scaffoldingmentioning

confidence: 99%

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Djisalov

Knežić

Podunavac

et al. 2021

Biology

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Meat cultivation via cellular agriculture holds great promise as a method for future food production. In theory, it is an ideal way of meat production, humane to the animals and sustainable for the environment, while keeping the same taste and nutritional values as traditional meat and having additional benefits such as controlled fat content and absence of antibiotics and hormones used in the traditional meat industry. However, in practice, there is still a number of challenges, such as those associated with the upscale of cultured meat (CM). CM food safety monitoring is a necessary factor when envisioning both the regulatory compliance and consumer acceptance. To achieve this, a multidisciplinary approach is necessary. This includes extensive development of the sensitive and specific analytical devices i.e., sensors to enable reliable food safety monitoring throughout the whole future food supply chain. In addition, advanced monitoring options can help in the further optimization of the meat cultivation which may reduce the currently still high costs of production. This review presents an overview of the sensor monitoring options for the most relevant parameters of importance for meat cultivation. Examples of the various types of sensors that can potentially be used in CM production are provided and the options for their integration into bioreactors, as well as suggestions on further improvements and more advanced integration approaches. In favor of the multidisciplinary approach, we also include an overview of the bioreactor types, scaffolding options as well as imaging techniques relevant for CM research. Furthermore, we briefly present the current status of the CM research and related regulation, societal aspects and challenges to its upscaling and commercialization.

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“…Besides the classical bioreactors made of glass or stainless steel, the fluid flows in small ( 87 ), bench top ( 88 ), and pilot scale ( 89 ), helping to identify, for example, death zones or stagnant zones, where fluid flows very slowly or does not flow at all ( 90 ), impacting in the mass transfer and in the final product viability. Li et al explored the CFD model to estimate the mass transfer and mixing performance of a reactor to scale-up cell production for cultured meat applications ( 91 ). The same approach is already widely used for the design and manufacture of several medical devices and is well-suited for conducting optimization studies to evaluate far more design alternatives than the build and test method, impacting in the reduction of design cycle time ( 15 , 67 ).…”

Section: Computational Fluid Dynamics Modeling For Scaling-up Cell Prmentioning

confidence: 99%

Scale-Up Technologies for the Manufacture of Adherent Cells

et al. 2020

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Great importance is being given to the impact our food supply chain and consumers' food habits are having on the environment, human health, and animal welfare. One of the latest developments aiming at positively changing the food ecosystem is represented by cultured meat. This form of cellular agriculture has the objective to generate slaughter-free meat products starting from the cultivation of few cells harvested from the animal tissue of interest. As a consequence, a large number of cells has to be generated at a reasonable cost. Just to give an idea of the scale, there were billions of cells just in a bite of the first cultured-meat burger. Thus, one of the major challenges faced by the scientists involved in this new ambitious and fascinating field, is how to efficiently scale-up cell manufacture. Considering the great potential presented by cultured meat, audiences from different backgrounds are very interested in this topic and eager to be informed of the challenges and possible solutions in this area. In light of this, we will provide an overview of the main existing bioprocessing technologies used to scale-up adherent cells at a small and large scale. Thus, giving a brief technical description of these bioprocesses, with the main associated advantages and disadvantages. Moreover, we will introduce an alternative solution we believe has the potential to revolutionize the way adherent cells are grown, helping cultured meat become a reality.

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A conceptual air-lift reactor design for large scale animal cell cultivation in the context of in vitro meat production

Cited by 75 publications

References 55 publications

Analysis of Scientific and Press Articles Related to Cultured Meat for a Better Understanding of Its Perception

Analysis of Scientific and Press Articles Related to Cultured Meat for a Better Understanding of Its Perception

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Scale-Up Technologies for the Manufacture of Adherent Cells

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