A conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application

Kandimalla, Vivek Babu; Tripathi, Vishal; Ju, Huangxian

doi:10.1016/j.biomaterials.2005.07.018

Cited by 137 publications

(74 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With increasing of enzyme level up to 5.0 mg mL À1 the catalytic current increased, the response then reduced slowly due to the increased protein concentration in biocomposite (Fig. 6b), which might be due to the fact that the increased protein content decreased the conductivity of the composite [38,39]. The concentration of 5.0 mg mL À1 was chosen as the GOD content for the preparation of the glucose biosensor.…”

Section: Optimization Of Enzyme Electrode Performancementioning

confidence: 99%

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

et al. 2009

View full text Add to dashboard Cite

A simple and controllable electrodeposition approach was proposed for one-step construction of glucose biosensors by in situ co-deposition of ferrocene-branched chitosan derivatives (CS-Fc), multiwalled carbon nanotubes (MWNTs), and glucose oxidase (GOD) onto electrode surface. The formation of CS-Fc could not only effectively prevent the leakage of Fc and retain its electrochemical activity, but also provide a biocompatible microenvironment for retaining the native activity of the immobilized biomolecules. Further entrapment of MWNTs into the CS matrix improved electronic conductivity of the biocomposite significantly. The facile procedure of immobilizing GOD and the promising feature of biocomposite will offer a versatile platform to fabricate biosensors and bioelectronic devices.

show abstract

Section: Optimization Of Enzyme Electrode Performancementioning

confidence: 99%

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Over the last two decades considerable attention has been paid to the development of new biocompatible materials with good conductivity, [1,2] suitable hydrophilicity, [1] high porosity, [3] and large surface area [4] for protein immobilization. The electrochemical biosensing applications of such materials have been explored due to the inherent advantages of electrochemical methods, such as low cost, portability, high sensitivity, and good selectivity.…”

Section: Introductionmentioning

confidence: 99%

Conductive Mesocellular Silica–Carbon Nanocomposite Foams for Immobilization, Direct Electrochemistry, and Biosensing of Proteins

Liu

2007

Adv Funct Materials

177

107

View full text Add to dashboard Cite

A mesocellular silica–carbon nanocomposite foam (MSCF) is designed for the immobilization and biosensing of proteins. The as‐prepared MSCF has a highly ordered mesostructure, good biocompatibility, favorable conductivity and hydrophilicity, large surface area, and a narrow pore‐size distribution, as verified by transmission electron microscopy (TEM), IR spectroscopy, electrochemical impedance spectroscopy (EIS), nitrogen adsorption–desorption isotherms, pore size distribution plots, and water contact angle measurements. Using glucose oxidase (GOD) as a model, the MSCF is tested for immobilization of redox proteins and the design of electrochemical biosensors. GOD molecules immobilized in the mesopores of the MSCF show direct electrochemistry with a fast electron transfer rate (14.0 ± 1.7 s–1) and good electrochemical performance. Based on a decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen, the proposed biosensor exhibits a linear response to glucose concentrations ranging from 50 μM to 5.0 mM with a detection limit of 34 μM at an applied potential of –0.4 V. The biosensor shows good stability and selectivity and is able to exclude interference from ascorbic acid (AA) and uric acid (UA) species that always coexist with glucose in real samples. The nanocomposite foam provides a good matrix for protein immobilization and biosensor preparation.

show abstract

“…It can be noted that BSA might be influencing the enzymatic reaction between the HRP and substrate by providing some favourable conditions to the HRP. Previous reports also suggested that some of the inert proteins could stabilise the active enzymes (Okawa et al, 1999;Kandimalla et al, 2006). Okawa et al (1999) reported that BSA can improve the thermostability of the enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…Okawa et al (1999) reported that BSA can improve the thermostability of the enzymes. The thermostability of glucose oxidase activity was improved after co-immobilisation with inert proteins inside the sol-gel (Kandimalla et al, 2006). Based on the comparison of blocking efficiency in PBS, 1.5% BSA was chosen as a suitable blocking solution.…”

Section: Resultsmentioning

confidence: 99%

Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay

Kandimalla¹,

Kandimalla²,

Hruška³

et al. 2007

Vet. Med.

View full text Add to dashboard Cite

ABSTRACT:During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. Hence, cost effective and simple screening methods are required for residual analysis of environmental and food samples onsite. Here, a simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase tracer conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 µg/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick.

show abstract

A conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application

Cited by 137 publications

References 32 publications

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

Conductive Mesocellular Silica–Carbon Nanocomposite Foams for Immobilization, Direct Electrochemistry, and Biosensing of Proteins

Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay

Contact Info

Product

Resources

About