Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay

Kandimalla, Vivek Babu; Kandimalla, N.; Hruška, K.; Fránek, Milan

doi:10.17221/2045-vetmed

Cited by 17 publications

(8 citation statements)

References 14 publications

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“…The enzyme-linked immunosorbent assay (ELISA) technique is one of the traditional techniques used to screen for the presence of antibiotic residues in meat, milk, surface water, groundwater, wastewater, soil and manure [30][31][32][33][34][35][36]. ELISA techniques are useful for the screening of structurally similar antibiotic mixtures in a sample [37].…”

Section: Introductionmentioning

confidence: 99%

Detection of Pharmaceutical Residues in Surface Waters of the Eastern Cape Province

Vumazonke

Khamanga

Ngqwala

2020

IJERPH

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Pharmaceuticals are emerging contaminants in the aquatic environments. Their presence poses toxicological effects in humans and animals even at trace concentrations. This study investigated the presence of antibiotics, anti-epilepsy and anti-inflammatory drugs in river water of selected rivers in the Eastern Cape Province in South Africa. Enzyme-linked immunosorbent assay was used for screening of sulfamethoxazole and fluoroquinolones antibiotics. The samples were collected in upper-stream, middle-stream and lower-stream regions of the rivers and effluent of selected wastewater treatment plants. Pre-concentration of the samples was conducted using lyophilisation and extraction was conducted using solid phase extraction (SPE) on Waters Oasis hydrophilic-lipophilic-balanced cartridge. The percentage recovery after sample clean-up on SPE was 103% ± 6.9%. This was followed by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry. The detected analytes were sulfamethoxazole, erythromycin, clarithromycin and carbamazepine. Carbamazepine and erythromycin were detected in high concentrations ranging from 81.8 to 36,576.2 ng/L and 11.2 to 11,800 ng/L respectively, while clarithromycin and sulfamethoxazole were detected at moderate concentrations ranging from 4.8 to 3280.4 ng/L and 6.6 to 6968 ng/L, respectively. High concentrations of pharmaceuticals were detected on the lower-stream sites as compared to upper-stream sites.

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Section: Introductionmentioning

confidence: 99%

Detection of Pharmaceutical Residues in Surface Waters of the Eastern Cape Province

Vumazonke

Khamanga

Ngqwala

2020

IJERPH

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“…Although currently available enzyme-linked immunosorbent assay (ELISA) methods were for individual SAs in waters (17,28), it should be possible to screen a broad spectrum of SAs in environmental waters by ELISA with antibodies that have a broadspecificity for SAs (29)(30)(31). So far, ELISA methods for analysis of environmental samples have suffered from limited sensitivity, low tolerance to matrix interferences, and variation of assay conditions (32)(33)(34)(35).…”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters

Zhang

Liu

Lei

et al. 2009

Environ. Sci. Technol.

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Using monoclonal antibodies labeled with Eu 3+ chelates, timeresolved fluoroimmunoassay (TRFIA) methods were developed for the determination of trace sulfamethazine (SMZ), sulfamethoxazole (SMX), and sulfadiazine (SDZ) in environmental waters. Under the optimized conditions, the developed methods offered (i) low detection limits (9.8 ng/L SMZ, 6.1 ng/L SMX, and 5.4 ng/L SDZ, based on 90% inhibition) which were about 1 order of magnitude lower than that of the enzyme-linked immunosorbent assay (ELISA), (ii) high selectivity with no crossreactivity (<0.05%) to similarly structured sulfonamides; (iii) high tolerance to variation of the sample pH (6.0-9.0) and salinity (0-100 mM), as well as the presence of humic acid (0-100 mg/L DOC) and heavy metals (0-1 mg/L concentration each of Cu 2+ , Cd 2+ , Hg 2+ , Pb 2+ , and As(V)) in the samples, and (iv) direct determination with low cost, high sample throughput, and low sample consumption (50-100 µL). The proposed TRFIA procedures were applied to determine sulfonamides in a variety of surface water and wastewater samples without sample pretreatment other than filtration. The satisfactory recoveries (64-127%) and reproducibilities (CV ) 0.2-16%) achieved, as well as the good agreement with those given by liquid chromatography-tandem mass spectroscopy and ELISA methods, demonstrated the applicability of the proposed TRFIA methods for routine screening/quantification of sulfonamides in environmental waters.

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“…To prove test validity, the implementation of an internal control zone (spot or line) into an immunochemical-based lateral flow test is a common practice. 11,12 So far, no control zone was implemented in column-based tests reported by ourselves. To the best of our knowledge, it was also not described by other groups.…”

Section: Control Layer Addition To the Column Testmentioning

confidence: 99%

New Immunochemically-based Field Test for Monitoring Benzo[α]pyrene in Aqueous Samples

et al. 2008

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Polycyclic aromatic hydrocarbons (PAHs) represent health and environmental concerns. The present study was designed to develop a highly sensitive and reliable immunochemically-based non-instrumental field assay to monitor the presence of benzo[a]pyrene (BAP) in aqueous samples at the ppt-level using visible test evaluation. The corresponding gel-based immunoassay was set up to combine preconcentration and detection of the target analyte using anti-PAH antibodies and horseradish-BAP tracer conjugate in one single cartridge. Water sample preparation, such as extraction, centrifugation, or filtering was found to be unnecessary. No interference by higher water-soluble PAHs at 4000-fold excess compared to BAP was observed. The assay was configured as a qualitative test (positive/negative) at the cut-off level of 5 ng L(-1), however, this level can be adapted to the required analyte concentration by rather simple adjustment of the anti-PAH antibody concentration of the test zone. Test validation was performed with real samples such as surface water, melted snow, and tap water using high performance liquid chromatography with fluorescent detection (HPLC-FLD) with solid phase extraction for validation.

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Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay

Cited by 17 publications

References 14 publications

Detection of Pharmaceutical Residues in Surface Waters of the Eastern Cape Province

Detection of Pharmaceutical Residues in Surface Waters of the Eastern Cape Province

Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters

New Immunochemically-based Field Test for Monitoring Benzo[α]pyrene in Aqueous Samples

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