A conserved module in the formation of moss midribs and seed plant axillary meristems

Ge, Yanhua; Gao, Yi; Jiao, Yuling; Wang, Ying

doi:10.1126/sciadv.add7275

Cited by 7 publications

(5 citation statements)

References 47 publications

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“…1 E and F ). The recent study also showed the loss of midrib in the double mutant ( 45 ). In wild type, medial cells divided periclinally to form midrib tissue, while in the ∆pplas1∆pplas2 double mutant, the same cells divided transversely or longitudinally, but not periclinally ( Fig.…”

Section: Resultsmentioning

confidence: 84%

GRAS transcription factors regulate cell division planes in moss overriding the default rule

Ishikawa

Fujiwara

Kosetsu

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Plant cells are surrounded by a cell wall and do not migrate, which makes the regulation of cell division orientation crucial for development. Regulatory mechanisms controlling cell division orientation may have contributed to the evolution of body organization in land plants. The GRAS family of transcription factors was transferred horizontally from soil bacteria to an algal common ancestor of land plants. SHORTROOT ( SHR ) and SCARECROW ( SCR ) genes in this family regulate formative periclinal cell divisions in the roots of flowering plants, but their roles in nonflowering plants and their evolution have not been studied in relation to body organization. Here, we show that SHR cell autonomously inhibits formative periclinal cell divisions indispensable for leaf vein formation in the moss Physcomitrium patens , and SHR expression is positively and negatively regulated by SCR and the GRAS member LATERAL SUPPRESSOR , respectively. While precursor cells of a leaf vein lacking SHR usually follow the geometry rule of dividing along the division plane with the minimum surface area, SHR overrides this rule and forces cells to divide nonpericlinally. Together, these results imply that these bacterially derived GRAS transcription factors were involved in the establishment of the genetic regulatory networks modulating cell division orientation in the common ancestor of land plants and were later adapted to function in flowering plant and moss lineages for their specific body organizations.

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Section: Resultsmentioning

confidence: 84%

GRAS transcription factors regulate cell division planes in moss overriding the default rule

Ishikawa

Fujiwara

Kosetsu

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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“…As observed in other cases, the function of TFs could be only conserved in the sporophyte generation ( Romani and Moreno 2021 ). In the case of GRAS TFs such as HAM and SCR, they seem to play a prominent role in the gametophytic stem cell organization in Physcomitrium, but putative orthologues for some do not exist in Marchantia ( Beheshti et al 2021 ; Ge et al 2022 ; Ishikawa et al 2023 ).…”

Section: Discussionmentioning

confidence: 99%

The landscape of transcription factor promoter activity during vegetative development in Marchantia

Romani,

Sauret-Güeto,

Rebmann

et al. 2024

The Plant Cell

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Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterising the transcriptional activity of TF genes in space and time is a critical step towards understanding complex biological systems. The vegetative gametophyte meristems of bryophytes share some characteristics with the shoot apical meristems of flowering plants. However, the identity and expression profiles of TFs associated with gametophyte organization are largely unknown. With only ∼450 putative TF genes, Marchantia (Marchantia polymorpha) is an outstanding model system for plant systems biology. We have generated a near-complete collection of promoter elements derived from Marchantia TF genes. We experimentally tested reporter fusions for all the TF promoters in the collection and systematically analysed expression patterns in Marchantia gemmae. This allowed us to build a map of expression domains in early vegetative development and identify a set of TF-derived promoters that are active in the stem cell zone. The cell markers provide additional tools and insight into the dynamic regulation of the gametophytic meristem and its evolution. In addition, we provide an online database of expression patterns for all promoters in the collection. We expect that these promoter elements will be useful for cell-type-specific expression, synthetic biology applications, and functional genomics.

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“…Very recently, Ge et al, and Ishikawa et al, have demonstrated the role of PpLAS (LATERAL SUPPRESSOR) and PpSHR (SHORT-ROOT) in moss leaf mid-vein development, respectively (Ge et al 2022;Ishikawa et al 2023).…”

Section: Discussionmentioning

confidence: 99%

“…The homologs of SCR and other GRAS domain containing proteins have been identified in P. patens (Engstrom 2011), and their role in asymmetric cell division is being studied (Ge et al 2022;Ishikawa et al 2023). Very recently, PpSHR has been shown to govern leaf vein formation in P. patens (Ishikawa et al 2023), and it is positively regulated by PpSCR1, however the study was limited to leaf primordium analysis.…”

Section: Introductionmentioning

confidence: 99%

PpSCARECROW1 (PpSCR1) regulates leaf blade and mid-vein development in Physcomitrium patens

Mohanasundaram,

Palit,

Bhide

et al. 2024

Plant Mol Biol

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In plants, asymmetric cell divisions result in distinct cell fates forming large and small daughter cells, adding to the cellular diversity in an organ. SCARECROW (SCR), a GRAS domain-containing transcription factor controls asymmetric periclinal cell divisions in flowering plants by governing radial patterning of ground tissue in roots and cell proliferation in leaves. Though SCR homologs are present across land plant lineages, the current understanding of their role in cellular patterning and leaf development is mostly limited to flowering plants. Our phylogenetic analysis identified three SCR homologs in moss Physcomitrium patens, amongst which PpSCR1 showed highest expression in gametophores and its promoter activity was prominent at the mid-vein and the flanking leaf blade cells pointing towards its role in leaf development. Notably, out of the three SCR homologs, only the ppscr1 knock-out lines developed slender leaves with four times narrower leaf blade and three times thicker mid-vein. Detailed histology studies revealed that slender leaf phenotype is either due to the loss of anticlinal cell divisions or failure of periclinal division suppression in the leaf blade. RNA-Seq analyses revealed that genes responsible for cell division and differentiation are expressed differentially in the mutant. PpSCR1 overexpression lines exhibited significantly wider leaf lamina, further reconfirming the role in leaf development. Together, our data suggests that PpSCR1 is involved in the leaf blade and mid-vein development of moss and that its role in the regulation of cell division and proliferation is ancient and conserved among flowering plants and mosses. Key messageThe GRAS domain containing protein PpSCR1 regulates asymmetric cell divisions and governs leaf blade and mid-vein development in moss Physcomitrium patens. KeywordsCell division • GRAS domain • Leaf shape and development • Physcomitrium patens • SCARECROW • Slenderleaf * Anjan K. Banerjee

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A conserved module in the formation of moss midribs and seed plant axillary meristems

Cited by 7 publications

References 47 publications

GRAS transcription factors regulate cell division planes in moss overriding the default rule

GRAS transcription factors regulate cell division planes in moss overriding the default rule

The landscape of transcription factor promoter activity during vegetative development in Marchantia

PpSCARECROW1 (PpSCR1) regulates leaf blade and mid-vein development in Physcomitrium patens

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