A Consortium of Three Enzymes: Xylanase, Arabinofuranosidase, and Cellulase from Aspergillus sp. which liquefied Coffee Pulp Wastes

Muzakhar, Kahar

doi:10.1088/1757-899x/546/2/022013

Cited by 13 publications

(13 citation statements)

References 20 publications

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“…Other studies mention that xylanase produced by Aspergillus sp. using coffee pulp waste substrate optimal at pH 5 and stable in the pH range of 3-6.5 [31]. This result indicates that the target enzyme can work actively in the acidic pH range and its activity decreases dramatically in alkaline or basic pH.…”

Section: E Characterization (Stability and Optimum Ph)mentioning

confidence: 78%

Cellulase, Pectinase, and Xylanase Production by Listeria sp. ISH 16 using Coffee Pulp Waste Medium

Wasilah

Muzakhar

Purwatiningsih

2021

IJPS

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In this study, microbial utilization of coffee pulp waste to produce three carbohydrases (cellulase, pectinase, and xylanase) has been investigated. An isolate Listeria sp. ISH-16 which grown in liquid medium containing 0.5% coffee pulp alkali extract can grow well with density of reach 10 7 x 58.5 cell/mL. Further, the culture can optimum produced of pectinase after 72 hours incubation at 37 o C, but for cellulase and xylanase longer 84 hours incubation needed. The harvested of crude pectinase, cellulase and xylanase had activity 0.15, 0.23, and 0.61 unit/ ml after dialysis, respectively. All enzymes stable at a range of pH 4-5.5, but they work optimum at different pH. Of each enzyme pectinase, cellulase, and xylanase work optimum at pH 5, 4.5, and 5.5. based on these results, it may be adopted as a strategy to produce enzymes with low cost so that further investigation such as large scale and purification enzyme is needed.

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Section: E Characterization (Stability and Optimum Ph)mentioning

confidence: 78%

Cellulase, Pectinase, and Xylanase Production by Listeria sp. ISH 16 using Coffee Pulp Waste Medium

Wasilah

Muzakhar

Purwatiningsih

2021

IJPS

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“…Finally, a mixture of three enzymes, xylanase, arabinofuranosidase and cellulase from Aspergillus sp. was shown to hydrolyze coffee pulp waste to simple sugars, with a hydrolysis grade of 79% (Muzakhar, 2019). A higher hydrolysis rate has been reported for coffee pulp subjected to solid state fermentation by Pestalotiosis sp.…”

Section: Other Biotechnological Usesmentioning

confidence: 98%

Coffee Pulp: An Industrial By-product with Uses in Agriculture, Nutrition and Biotechnology

Marín-Tello

Lorena

Alexander

et al. 2020

RAS

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“…One ml supernatant was measured using a spectrophotometer at a wavelength of 500 nm [14,21]. One unit of enzyme activity was defined as the amount of enzyme that produced reducing sugar at a rate of 1 µmol per minute [15,22].…”

Section: Enzyme Assaysmentioning

confidence: 99%

Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

Azizah¹,

Hidayah²,

Amelia³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Hydrolytic Pectinase is an important enzyme and is widely used in several industries. In the previous investigation, this enzyme could be produced by Aspergillus sp. VTM5 under solid-state fermentation and the coffee pulp waste substrates were used. The specific activity of about 0.4 U/ml of pectinase was detected when 40gr coffee pulp water-saturated inoculated with 1.2x10 7 spores of Aspergillus sp. VTM5 was incubated at 30 °C for 168 h, and without any nutrients added. Against 0.5% alkaline extract of pomelo pulp substrate in 20 Mm acetate buffer pH 5, pectinase activity increases up to 1.03 U/ml, and the purity 3.5 times, after dialyzed using 12-14 kDa, pores cellulose membrane tube higher than previously step. Open column purification using Fractogel EMD SO3-(S) cation exchange chromatography, pectinase was eluted at 0.45 M NaCl. The pectinase activity significantly increased to 0.295 U/ml with 12.37-fold and 17.04% yield. Based on these results, it can be proved that coffee pulp waste is suitable as a substrate for pectinase production by Aspergillus sp. VTM5. Further research to analyze the properties and characterize the stability of pectinase against temperature, pH, the effect of heavy metals, and molecular analysis is needed.

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A Consortium of Three Enzymes: Xylanase, Arabinofuranosidase, and Cellulase from Aspergillus sp. which liquefied Coffee Pulp Wastes

Cited by 13 publications

References 20 publications

Cellulase, Pectinase, and Xylanase Production by Listeria sp. ISH 16 using Coffee Pulp Waste Medium

Cellulase, Pectinase, and Xylanase Production by Listeria sp. ISH 16 using Coffee Pulp Waste Medium

Coffee Pulp: An Industrial By-product with Uses in Agriculture, Nutrition and Biotechnology

Pectinase Production of Aspergillus sp. VTM5 Through Solid State Fermentation Using Coffee Pulp Substrate and Its Purification

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