Cellulase, Pectinase, and Xylanase Production  by Listeria sp. ISH 16 using Coffee Pulp  Waste Medium

Wasilah, Ummi; Muzakhar, Kahar; Purwatiningsih, Purwatiningsih

doi:10.12962/j23546026.y2020i6.11122

Cited by 4 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sample molecules larger than membrane pores will be retained on the sample side of the membrane. However, smaller molecules and buffers will pass through the membrane freely, reducing the concentration of molecules in the sample (Wasilah et al 2020). The dialysis step was carried out in a cold environment at 4ºC to avoid decreased enzyme activity.…”

Section: Discussionmentioning

confidence: 99%

Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads

UTAMI

Fahrurrozi²,

Meryandini³

2022

Biodiversitas

View full text Add to dashboard Cite

Abstract. Utami AP, Fahrurrozi, Meryandini A. 2022. Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads. Biodiversitas 23: 3960-3966. Pectinase is one of the enzymes often used in the industrial sector, especially in the food industry, such as extracting and clarifying juices. Previous studies have obtained pectinolytic microbial cultures of Bacillus sp. 2P11 isolated from cocoa beans. Pectinase production using pure pectin substrates such as citrus pectin or pectin from apples costs a lot because these pectins are expensive. Liquid substrate from cocoa pods TSH 858 and ICS 60 with a concentration of 10% is a good amount for pectinase production, and the percentage of total pectin from cocoa pods is 0.823%. The amount of activity of crude extract of pectinase enzyme is 416,780 mU/mL. Precipitation with ammonium sulfate and dialysis increased pectinase activity by 452,335 mU/mL and 586.88 mU/mL, respectively. Enzymes can be used repeatedly with enzyme immobilization. In this study, the immobilization of the enzyme entrapment method with sodium alginate and CaCl2 was designed with the response surface method. The optimum concentration of sodium alginate was 0.5% and 0.3 M CaCl2, with the percentage of an immobilized enzyme at 40.782%. Stability and repeated use of immobilized pectinase can be done up to 5 times.

show abstract

Section: Discussionmentioning

confidence: 99%

Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads

UTAMI

Fahrurrozi²,

Meryandini³

2022

Biodiversitas

View full text Add to dashboard Cite

show abstract

“…Small molecules will diffuse across the membrane, while the larger would retain [8]. It was also demonstrated in another study that using dialysis can increase the purity of cellulase crude extract produced from solid-state fermentation [19].…”

Section: Purification Of Cellulasementioning

confidence: 92%

An Extracellular Cellulase Production Under Solid-State Fermentation of Coffee Pulp Waste by Aspergillus sp. VTM1 and Its Purification

Putrasetya¹,

Rusdianti²,

Sugianto³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

Self Cite

View full text Add to dashboard Cite

Microbial utilization of agricultural biomass wastes has been widely used to synthesize certain an enzyme. We previously investigated that cellulase can be produced under solid stated fermentation based on coffee pulp waste substrate using Aspergillus sp. VTM1. Crude cellulase production was optimized for a week of incubation at 30 °C, the crude was harvested daily with distilled water containing 1% NaCl and 0.1% NaN3. To measure cellulase activity, the reducing sugar Somogy-Nelson method was used. In the next step, crude cellulase was dialyzed using a 45μm cellulose membrane tube 12-14 kDa against acetate buffer pH 5. Further analysis found that the maximum activity of cellulase production was obtained after inoculating 10 8 spores/ml of Aspergillus sp. VTM1 to the 5g of sterile coffee pulp (67% humidity) at 30 °C for 96 h. Large-scale production of cellulase crude extract results in an activity of 0.70 U/ml with a total activity of 121.1 U/ml. After the dialysis, the enzyme activity (0.81 U/ml) and specific activity (0.01 U/mg) were increased compared to the crude extract. On an anion exchanger using DEAE cellulose DE-52, the specific activity was 0.1 U/mg, increased 25.03 times with a yield of 36,82%. The findings of this study lead to a green strategy for cellulase production under solid-state fermentation by Aspergillus sp. VTM1 and its purification.

show abstract

“…The preculture was maintained at optimum conditions, as mentioned in our previous research (Wasilah et al, 2020). A nutrient agar broth medium was used, and the cell density reached 10 8 /ml after being shaken at 100 rpm, 24 hours incubation at 30 o C. This culture was used as an inoculum in SSF for pectinase production.…”

Section: Maintainance Of Ish16 Isolate Preculture For Pectinase Produ...mentioning

confidence: 99%

“…Solid state fermentation for pectinase production was monitored for a period of 1-7 days by measuring the pectinase activity against 1% pectin substrate in 20 mM acetate buffer pH5. Optimizing water content during SFF in pectinase production is also carried out using the same previous research method (Wasilah et al, 2020). Jurnal Biodjati 7(2):177-188, November 2022 http://journal.uinsgd.ac.id/index.php/biodjati…”

Section: Solid State Fermentation and Pectinase Productionmentioning

confidence: 99%

“…is potential pectinolytic-producing bacteria that have been previously isolated. Under submerged fermentation in the alkaline extract of the coffee pulp at 10%, the pectinase activity reaches 0.52 U/ml (Azizah, 2019;Wasilah et al, 2020). Utilizing biomass by involving microbes is one of the strategies to effectively reduce costs and be environmentally friendly so that there is an opportunity for further research on producing pectinase from ISH16 bacteria isolate based on coffee pulp waste.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate

et al. 2022

View full text Add to dashboard Cite

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.

show abstract

Cellulase, Pectinase, and Xylanase Production by Listeria sp. ISH 16 using Coffee Pulp Waste Medium

Cited by 4 publications

References 19 publications

Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads

Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads

An Extracellular Cellulase Production Under Solid-State Fermentation of Coffee Pulp Waste by Aspergillus sp. VTM1 and Its Purification

An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate

Contact Info

Product

Resources

About