Since 1997, scientists have been trying to utilize new non-invasive approaches for thermal discomfort detection, which promise to be more effective for comparing frameworks that need direct responses from users. Due to rapid technological development in the bio-metrical field, a systematic literature review to investigate the possibility of thermal discomfort detection at the work place by non-invasive means using bio-sensing technology was performed. Firstly, the problem intervention comparison outcome context (PICOC) framework was introduced in the study to identify the main points for meta-analysis and, in turn, to provide relevant keywords for the literature search. In total, 2776 studies were found and processed using the preferred reporting items for systematic reviews and meta-analyses (PRISMA) methodology. After filtering by defined criterion, 35 articles were obtained for detailed investigation with respect to facility types used in the experiment, amount of people for data collection and algorithms used for prediction of the thermal discomfort event. The given study concludes that there is potential for the creation of non-invasive thermal discomfort detection models via utilization of bio-sensing technologies, which will provide a better user interaction with the built environment, potentially decrease energy use and enable better productivity. There is definitely room for improvement within the field of non-invasive thermal discomfort detection, especially with respect to data collection, algorithm implementation and sample size, in order to have opportunities for the deployment of developed solutions in real life. Based on the literature review, the potential of novel technology is seen to utilize a more intelligent approach for performing non-invasive thermal discomfort prediction. The architecture of deep neural networks should be studied more due to the specifics of its hidden layers and its ability of hierarchical data extraction. This machine learning algorithm can provide a better model for thermal discomfort detection based on a data set with different types of bio-metrical variables.