A continuous-flow acoustofluidic cytometer for single-cell mechanotyping

Wang, Han; Liu, Zhongzheng; Shin, Dong M.; Chen, Zhuo G.; Cho, Younghak; Kim, Yong-Joe; Han, Arum

doi:10.1039/c8lc00711j

Cited by 31 publications

(23 citation statements)

References 44 publications

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“…For example, using inertial microfluidics, white blood cells could be hydrodynamically isolated from lysed blood containing ring-stage malaria parasites as a result of the white blood cells' larger sizes [128]. Other recent works that demonstrate potential applications for single-cell label-free infectious disease analysis includes cell identification via their acoustophoretic responses [129], as well as their deformability and hydrodynamic resistance [130].…”

Section: Label-free Analysismentioning

confidence: 99%

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Lin

Tay

Lu³

et al. 2020

Cells

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The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell Sorting (FACS) have helped show substantial links between cellular heterogeneity and infectious disease progression. The extensive characterization of genomic and phenotypic biomarkers, in addition to host–pathogen interactions at the single-cell level, has resulted in the discovery of previously unknown infection mechanisms as well as potential treatment options. In this article, we review the various single-cell technologies and their applications in the ongoing fight against infectious diseases, as well as discuss the potential opportunities for future development.

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Section: Label-free Analysismentioning

confidence: 99%

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Lin

Tay

Lu³

et al. 2020

Cells

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“…The microfluidics acoustic-based devices benefit from acoustic radiation force for micro/nanoparticle confinement at predefined locations in a continuous flow. There are two main acoustic-based separation techniques, bulk acoustic wave (BAW) based systems ( Leibacher et al, 2015 ), and surface acoustic wave (SAW) based systems ( Wang et al, 2019a ), ( Fu et al, 2017 ). In SAW-based microdevices, a pair of interdigitated transducers electrodes are patterned at two sides of the fluidic channel, generating two opposite-direction surface acoustic waves by the piezoelectric surface actuation.…”

Section: Introductionmentioning

confidence: 99%

Neural Network-Based Optimization of an Acousto Microfluidic System for Submicron Bioparticle Separation

Talebjedi

Heydari

Taatizadeh

et al. 2022

Front. Bioeng. Biotechnol.

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The advancement in microfluidics has provided an excellent opportunity for shifting from conventional sub-micron-sized isolation and purification methods to more robust and cost-effective lab-on-chip platforms. The acoustic-driven separation approach applies differential forces acting on target particles, guiding them towards different paths in a label-free and biocompatible manner. The main challenges in designing the acoustofluidic-based isolation platforms are minimizing the reflected radio frequency signal power to achieve the highest acoustic radiation force acting on micro/nano-sized particles and tuning the bandwidth of the acoustic resonator in an acceptable range for efficient size-based binning of particles. Due to the complexity of the physics involved in acoustic-based separations, the current existing lack in performance predictive understanding makes designing these miniature systems iterative and resource-intensive. This study introduces a unique approach for design automation of acoustofluidic devices by integrating the machine learning and multi-objective heuristic optimization approaches. First, a neural network-based prediction platform was developed to predict the resonator’s frequency response according to different geometrical configurations of interdigitated transducers In the next step, the multi-objective optimization approach was executed for extracting the optimum design features for maximum possible device performance according to decision-maker criteria. The results show that the proposed methodology can significantly improve the fine-tuned IDT designs with minimum power loss and maximum working frequency range. The examination of the power loss and bandwidth on the alternation and distribution of the acoustic pressure inside the microfluidic channel was carried out by conducting a 3D finite element-based simulation. The proposed methodology improves the performance of the acoustic transducer by overcoming the constraints related to bandwidth operation, the magnitude of acoustic radiation force on particles, and the distribution of pressure acoustic inside the microchannel.

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“…Recently, acoustic tweezers have garnered increased interest from the biomedical research community, as they can perform noncontact, label-free, and precise manipulation of bioparticles (1,(8)(9)(10)(11). With these merits, acoustic tweezers have been used in a wide range of biomedical applications, including patterning and printing cells (12)(13)(14)(15), separating and sorting cells (16)(17)(18)(19), controlling cell-cell interactions (20,21), single-cell analysis (22)(23)(24), concentrating bioparticles (25)(26)(27)(28)(29)(30)(31), acousto-mechanical phenotyping (32,33), constructing tissues (34)(35)(36)(37)(38), generating and translating droplets (39)(40)(41), rotating multicellular organisms (42,43), and isolating extracellular vesicles (44,45).…”

Section: Introductionmentioning

confidence: 99%

Generating multifunctional acoustic tweezers in Petri dishes for contactless, precise manipulation of bioparticles

et al. 2020

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Acoustic tweezers are a promising technology for the biocompatible, precise manipulation of delicate bioparticles ranging from nanometer-sized exosomes to millimeter-sized zebrafish larva. However, their widespread usage is hindered by their low compatibility with the workflows in biological laboratories. Here, we present multifunctional acoustic tweezers that can manipulate bioparticles in a disposable Petri dish. Various functionalities including cell patterning, tissue engineering, concentrating particles, translating cells, stimulating cells, and cell lysis are demonstrated. Moreover, leaky surface acoustic wave–based holography is achieved by encoding required phases in electrode profiles of interdigitated transducers. This overcomes the frequency and resolution limits of previous holographic techniques to control three-dimensional acoustic beams in microscale. This study presents a favorable technique for noncontact and label-free manipulation of bioparticles in commonly used Petri dishes. It can be readily adopted by the biological and medical communities for cell studies, tissue generation, and regenerative medicine.

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A continuous-flow acoustofluidic cytometer for single-cell mechanotyping

Abstract: A continuous-flow single-cell mechanotyping method which can decouple the cell size-dependent effect from the cell compressibility-dependent effect is presented.

Cited by 31 publications

References 44 publications

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Neural Network-Based Optimization of an Acousto Microfluidic System for Submicron Bioparticle Separation

Generating multifunctional acoustic tweezers in Petri dishes for contactless, precise manipulation of bioparticles

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