A contribution to the innervation of the urinary bladder of the cat

Edge, N. D.

doi:10.1113/jphysiol.1955.sp005237

Cited by 57 publications

(18 citation statements)

References 12 publications

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“…The atropineresistance of responses of the bladder to nerve stimulation is common to many species; it has been observed in the dog, cat and rabbit (Langley & Anderson, 1895;Henderson & Roepke, 1935;Ursillo & Clark, 1956;Ursillo, 1961;Edge, 1955) and in the possum (Burnstock & Campbell, 1963). These observations may indicate that the nerves were not cholinergic; however, Ambache (1955) was of the opinion that failure of atropine to block responses to nerve stimulation is not in itself proof that the nerves are noncholinergic.…”

Section: Discussionmentioning

confidence: 99%

Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

Huković

Rand

Vanov

1965

British Journal of Pharmacology and Chemotherapy

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Section: Discussionmentioning

confidence: 99%

Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

Huković

Rand

Vanov

1965

British Journal of Pharmacology and Chemotherapy

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“…In species other than the rat, contractions of the bladder have been observed on stimulation of the pelvic nerve, hypogastric nerve (see Gruber, 1933;Langley & Anderson, 1895;Elliot, 1907;Edge, 1955;Garret, 1963) and even the vagus nerves (Oehl, 1865, andPalumordwinov, 1916;cited in Gruber, 1933). The absence of responses of the bladder to stimulation of the vagus in the rat shows that in this species the vagus does not contribute motor fibres to the bladder.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies on mammalian urinary bladder have been made using dogs or cats with the bladder in situ (Langley & Anderson, 1895;Elliot, 1907;Edmunds & Roth, 1920;Henderson & Roepke, 1935;Edge, 1955;Gyermek, 1961). Responses to drugs and to nerve stimulation have usually been recorded by measuring changes in the intravesical pressure.…”

Section: Discussionmentioning

confidence: 99%

“…Elliot (1907) reported considerable species differences in the innervation of the bladder. The responses of the urinary bladder of several mammalian species (dog, cat, rabbit and ring-tail possum) to drugs and to nerve stimulation have been investigated by Edmunds & Roth (1920), Henderson & Roepke (1935), Edge (1955), Ursillo & Clark (1956) and Burnstock & Campbell (1963).The present paper describes the actions of acetylcholine, nicotine, dimethylphenylpiperazinium and 5-hydroxytryptamine on the rat urinary bladder in situ. Responses of the bladder were elicited by stimulation of its motor nerves and compared with the responses produced by drugs.…”

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RESPONSES OF THE RAT URINARY BLADDER IN SITU TO DRUGS AND TO NERVE STIMULATION

Vanov

1965

British Journal of Pharmacology and Chemotherapy

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The autonomic innervation of the urinary bladder has been studied extensively. Langley & Anderson (1895) first showed that stimulation of the pelvic nerves produced contraction of the bladder. Elliot (1907) reported considerable species differences in the innervation of the bladder. The responses of the urinary bladder of several mammalian species (dog, cat, rabbit and ring-tail possum) to drugs and to nerve stimulation have been investigated by Edmunds & Roth (1920), Henderson & Roepke (1935), Edge (1955), Ursillo & Clark (1956) and Burnstock & Campbell (1963).The present paper describes the actions of acetylcholine, nicotine, dimethylphenylpiperazinium and 5-hydroxytryptamine on the rat urinary bladder in situ. Responses of the bladder were elicited by stimulation of its motor nerves and compared with the responses produced by drugs. The results indicate that the autonomic innervation of the urinary bladder of the rat has characteristics of its own. METHODSRats of either sex weighing 180 to 250 g were used. They were anaesthetized by pouring a few drops of halothane on cotton wool in a Perspex box (dimensions 20 x 15 x 15 cm). Complete anaesthesia was obtained within 5 min. The rats were not atropinized because halothane, unlike ether, did not cause mucous secretion. A cannula was inserted into the trachea, the rats were pithed as described by Shipley & Tilden (1947) and immediately put on artificial ventilation. A polyethylene cannula was inserted into a femoral vein for injecting drugs. The blood pressure was recorded from a carotid artery with either a Condon manometer or a pressure transducer (Devices).The lower abdomen was opened along the midline, and the bladder exposed and detached from the prostate gland by blunt dissection. A thread attached to the vertex was fixed to a lever or to a straingauge for recording isotonic contractions, at a tension of 1.5 to 2 g, on a kymograph or a pen-recorder. Cotton-wool swabs soaked with warm saline were laid around the bladder to keep it warm and moist. The vesical nerves were stimulated by shielded platinum electrodes put underneath a ureter a few millimetres from the bladder. Rectangular pulses of 1 to 2 msec duration, and 5 to 10 V strength, generated from a Palmer electronic stimulator, were applied at a frequency of 10 or 20 shocks/sec, for 10 sec every 2 min.The hypogastric nerve was stimulated at about 1 cm from the bladder with pulses of 2 msec duration, 5 to 10 V strength and a frequency of 10 to 50 shocks/sec. In several experiments the right cervical vagus was stimulated. The stimuli applied were of the same duration, strength and frequency as those for the vesical nerves.The nerves were not cut, but stimulation was in effect only of centrifugal fibres, because the central nervous system in these animals had been destroyed by pithing.

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Antimuscarinic properties of vamicamide, a novel compound for the treatment of pollakiuria

Yamamoto

Matsuo

Yamazaki

et al. 1995

Drug Development Research

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The antimuscarinic profile of vamicamide, a novel compound for the treatment of pollakiuria, was investigated in both in vitro and in vivo preparations. inhibited the contractile response of isolated guinea-pig detrusor muscle to transmural electrical stimulation. In isolated guinea-pig detrusor muscle, vamicamide also inhibited contractile response to carbachol with an IC,, value of 0.71 pM, whereas it had little or no effect on detrusor contractions induced by KCI, BaCI,, or a, p-methylene ATP. In binding studies with dog membrane preparations, the dissociation constant (Ki) of vamicamide against tritiated N-methylscopolamine ([3HlNMS) binding was 172 * 8 n M for the urinary bladder, which was lower (P < 0.01) than those for the heart (322 ? 31 nM), stomach (317 & 29 nM), and salivary gland (321 * 32 nM). The Ki value for the cerebral cortex (95 * 4 nM) tended to be lower than that for the urinary bladder. In binding studies with cloned human muscarinic receptor subtypes (ml-m3), the dissociation constant (Ki) of vamicamide against I3H]NMS binding was 89.0 +. 3.5 n M for the m3 receptor subtype, which was lower than those for the m l (235 ? 5 nM, P < 0.05) and m2 (593 ? 49 nM, P < 0.01) receptor subtypes. In anesthetized dogs, vamicamide (3.2-100 Fg/kg; 11-336 nmol/kg, iv) inhibited the carbachol-induced contractile responses of the urinary bladder with an I D, , value of 16.1 pg/kg (54 nmol/kg), the stomach with a value of 31.3 pg/kg (105 nmol/kg), the descending colon with a value of 10.1 pg/kg (34 nmol/kg), and secretory response of the salivary gland to carbachol with a value of 43.3 pg/kg (146 nmol/kg); the inhibitory effects of the compound on the stomach and salivary gland were weaker (P < 0.01) than that on the urinary bladder. Furthermore, duration of the action of vamicamide was longer on the urinary bladder and descending colon than those on the stomach and salivary gland. These results suggest that vamicamide has a selective binding affinity to the muscarinic m3 receptor subtype and exhibits greater and longer inhibitory action on the urinary bladder than the other organs examined. o 1995 Wiiey-Liss, Inc.

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A contribution to the innervation of the urinary bladder of the cat

Cited by 57 publications

References 12 publications

Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

RESPONSES OF THE RAT URINARY BLADDER IN SITU TO DRUGS AND TO NERVE STIMULATION

Antimuscarinic properties of vamicamide, a novel compound for the treatment of pollakiuria

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