A controlled trial of the effect of milk basic protein (MBP) supplementation on bone metabolism in healthy menopausal women

Abstract

Milk has more beneficial effects on bone health than other food sources. Recent in vitro and in vivo studies have shown that milk whey protein, especially its basic protein fraction (milk basic protein, MBP), contains several components capable of promoting bone formation and inhibiting bone resorption. The object of this study was to examine the effect of MBP on the bone metabolism of healthy menopausal women. Thirty-two healthy menopausal women were randomly assigned to treatment with either placebo or MBP (40 mg per day) for 6 months. The bone mineral density (BMD) of the lumbar vertebrae L2-L4 of each subject was measured by dual-energy X-ray absorptiometry (DXA) at 0 and 6 months of treatment. Serum and urine indices of bone metabolism were measured at 0, 3 and 6 months. Twenty-seven subjects who completed the study in accordance with the protocol were included in the analysis. The mean rate of gain of lumbar BMD in the MBP group (1.21%) was significantly higher than in the placebo group (-0.66%, P=0.046). When compared with the placebo group, urinary cross-linked N-telopeptides of type-I collagen (NTx) were significantly decreased in the MBP group at 6 months, but no significant difference in serum osteocalcin was observed between the two groups. The urinary NTx excretion was found to be related to serum osteocalcin in the MBP group at 3 and 6 months, indicating that MBP maintained the balance of bone remodeling. These results suggested that MBP supplementation was effective in preventing bone loss in menopausal women and that this improvement in BMD may be primarily mediated through the inhibition of bone resorption while maintaining the balance of bone remodeling by MBP supplementation.

Dietary protein and bone health: a systematic review and meta-analysis from the National Osteoporosis Foundation,

“…Ten RCTs examined osteocalcin concentrations (Supplemental Table 6) (25,27,28,30,34,35,37,38,61,62). Eight studies (25,27,28,30,35,37,61,62) prescribed milk proteinbased supplements: the doses of milk-based protein in the higher-protein groups in 7 studies ranged from 10 to 45 g/d and 1 study (62) supplemented 250-mg capsules of milk ribonucleaseenriched lactoferrin/d, whereas doses in the lower-protein groups ranged from 0 to 2.1 g milk-based protein/d.…”

Invited review: Dairy intake and bone health: A viewpoint from the state of the art

“…Milk whey protein, especially the fraction with alkaline isoelectric point (milk basic protein, MBP), suppressed bone resorption and prevented bone loss caused by ovariectomy in aged rats (Toba et al, 2000). Supplementation with MBP also increased bone mineral density and suppressed urine levels of bone resorption biomarkers in young women (Aoe et al, 2001;Yamamura et al, 2002;Itabashi, 2006;Uenishi et al, 2007), in menopausal women (Aoe et al, 2005) and in healthy older women (>65 yr; Aoyagi et al, 2010). In particular, MBP directly suppressed osteoclast-mediated bone resorption and led to decreased osteoclast number in animal studies (discussed in Uenishi et al, 2007).…”

Concentrated Bovine-Milk Whey Active Protein (CBP) Supplement-Combined Dynamic Flamingo Therapy (DFT) Activates Bone Metabolism and Bone-Related Factors

“…In other words, DPD exhibits a biological reaction regardless of the type of exercise, and the difference in the regression time is influenced by the intensity of the exercise. Aoe et al reported that a 6-month intake of MBP supplements resulted in a reduction in type I collagen cross-linked N-telopeptides (NTx) and suggested that bone improvement was suppressed by bone-resorbing factors [9,10]. Research by Zou et al [11] also showed a reduction in NTx, despite a constant BAP, after MBP supplementation for 8 months.…”

“…In recent years, consumption of milk basic protein (MBP) supplement and similar dairy products has frequently been used in order to promote bone improvement [9][10][11][12][13]. Of these products, concentrated bovine-milk whey active protein (CBP) has been shown to induce bone formation in vitro, and has attracted interest as a method of bone improvement [12,13].…”