A convenient and label-free colorimetric assay for dopamine detection based on the inhibition of the Cu(ii)-catalyzed oxidation of a 3,3′,5,5′-tetramethylbenzidine–H₂O₂ system

Abstract

A convenient and label-free colorimetric assay was reported for dopamine detection based on the inhibition of the Cu2+ catalyzed oxidation of a TMB–H2O2 system.

Protein–inorganic nano hybrid sheets of Pd embedded BSA as a robust catalyst in water for oxidase mimic activity and C–C coupling reactions, and as a sustainable material for micromolar sensing of dopamine

“…Abnormal levels of dopamine are responsible for life threatening disorders like Parkinson's disease, 29 dementia and clinical depression. 30 Although there are several literature reports for efficient detection of dopamine, [31][32][33] a facile colorimetric method for its sensing in bodily fluids with no necessity of H 2 O 2 is always in demand. Utilizing the oxidase activity of Pd_NP@BSA sheet , a colorimetric method has been developed using the Pd_NP@BSA sheet _TMB ox system for easy detection of dopamine in biological samples such as human serum and urine.…”

Development of Enzyme Conductometric Biosensor for Dopamine Determination in Aqueous Samples

“…Common analytical methods of the dopamine determination are high performance liquid chromatography coupled with electrochemical or other detectors, spectrofluorimetry, mass spectrometry and spectrophotometry [11]. Additionally, other analytical methods have been developed, such as colorimetric assays, fluorescent assays, and photonic sensors [12][13][14][15][16]. However, these methods require complex sample pretreatment and expensive equipment.…”

A Convenient and Label-Free Colorimetric Detection for L-Histidine Based on Inhibition of Oxidation of 3,3′,5,5′-Tetramethylbenzidine-H2O2 System Triggered by Copper Ions

“…Inexpensive, stable, and readily available Cu 2+ ions are good candidates as peroxidase mimetics. Analytical systems comprising TMB, Cu 2+ ions, and H 2 O 2 were used to detect uric acids ( Lu et al, 2017 ), dopamine ( Wang et al, 2017 ), and glucose ( Li et al, 2019 ) in biological samples. During the analytical process, analytes are catalytically oxidized in the presence of enzymes to generate H 2 O 2 , which induces a color reaction.…”

“…Because of the presence of the N-coordinating ligands of the imidazole ring and –COOH groups, L-his presents a remarkable affinity for Cu 2+ ions and can strongly chelate with Cu 2+ ions to form stable L-His–Cu 2+ complexes ( Elbaz et al, 2008 ; Zhang et al, 2013 ; Wang et al, 2017 ; Cai et al, 2020 ). Upon adding L-His to the assay, the Cu 2+ ion-catalyzed TMB oxidation to oxTMB in the presence of H 2 O 2 was inhibited.…”