A Copper Oxide/Zinc Oxide Composite Nano-Surface for Use in a Biosensor

Abstract

In this study, biosensors based on zinc oxide–copper oxide composite nano-surfaces were prepared using a simple and inexpensive distributed colloidal technique. Combinations of mixed dispersions with volume ratios of 1:1, 1:2 and 2:1 ZnO:CuO were compared. The uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) were analysed using scanning electron microscopy (SEM), Atomic Force Microscopy (AFM) and Raman Spectroscopy. The ZnO–CuO composite biosensor nano-surfaces showed a significantly increased impedimetric signal compared with pure ZnO nanocrystals, and the maximum output was achieved with a volume ratio of 1:2 ZnO/CuO. The antibody capture of C-reactive protein (CRP) on the nano-surfaces was used to demonstrate the enhanced signal generated with increasing amounts of CuO in the nano-surface.

Flexible ZnO-mAb nanoplatforms for selective peripheral blood mononuclear cell immobilization

“…In fact, Wang et al have concluded that ZnO NRs provided more protein interactions than other ZnO nanostructured materials 37 , reinforcing the development of ZnO NR-based immune-dependent platforms. For instance, Cao and colleagues have developed ZnO NRs-based sensors to immunodetect C-reactive protein, where a colloidal dispersion technique was used to cover PET 4,38 . Similarly, Tamashevski et al 15 developed a ZnO NRs-based sensor towards the immunodetection of human leukemic cells, where gaseous-dispersive synthesis was used to cover a glass substrate.…”

Nanoplatforms for Sepsis Management: Rapid Detection/Warning, Pathogen Elimination and Restoring Immune Homeostasis

“…More importantly, the nanosurface-based nanodiagnostic platform is easy to fabricate inexpensively [ 145 ], which implies excellent possibilities for clinical translation. Alternatively, the incorporation of copper oxide (CuO) nanocrystals could improve the performance of zinc oxide (ZnO) nanocrystal-based biosurfaces by potentiating redox properties and electron transfer and decreasing band gap energy [ 143 , 172 ]. For instance, biosurfaces fabricated by ZnO–CuO hybrid nanomaterials with a volume ratio of 1:2 exhibited a dramatically enhanced signal for CRP detection in comparison with pure ZnO-based biosurfaces (Fig.…”

“…For instance, biosurfaces fabricated by ZnO–CuO hybrid nanomaterials with a volume ratio of 1:2 exhibited a dramatically enhanced signal for CRP detection in comparison with pure ZnO-based biosurfaces (Fig. 6 c) [ 143 ]. Consequently, more accurate, time-saving and adaptive methods for clinical applications can be developed by precisely designing and optimizing the formulations of nanocrystal-based diagnostic platforms, including nanocrystal species, multiple components, sizes, and ratios.…”

A Review of Microwave Synthesis of Zinc Oxide Nanomaterials: Reactants, Process Parameters and Morphologies

“…It has been proved that ZnO NPs applied in textiles effectively fight bacteria, e.g., Staphylococcus aureus or Klebsiella pneumoniae [ 182 ]. In medicine, research is in progress on the application of ZnO NMs as a potential contrast agent (imaging), drug carrier, iron delivery, gene carrier, biosensor, a potential anti-cancer agent, in a photodynamic therapy, for prophylactic and therapeutic vaccines, support for antifungal treatments, in photocatalytic antibiotics, inhibition of influenza virus infection, diagnostic-therapeutic functions, wound dressing and tissue engineering [ 70 , 166 , 168 , 183 , 184 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 , 195 , 196 , 197 , 198 , 199 , 200 , 201 , 202 , 203 , 204 , 205 , 206 , 207 , 208 , 209 , 210 , 211 , 212 , 213 ]. However, before implementing ZnO NMs in biomedical applications on a commercial scale, the toxicity of ZnO NMs must be carefully learnt and their toxicity mechanisms must be explained [ 121 , 162 , 171 , 174 , 178 , ...…”