A core region of the mafK gene IN promoter directs neurone‐specific transcription in vivo

Motohashi, Hozumi; Ohta, Jun; Engel, James Douglas; Yamamoto, Masayuki

doi:10.1046/j.1365-2443.1998.00222.x

Cited by 12 publications

(9 citation statements)

References 58 publications

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“…1C). This is consistent with the absence of tags corresponding to MafK in our SAGE analysis of long-term (9 d) NGF-treated cells (Table 1) and with the report by Motohashi et al (1998) Semiquantitative PCRs were performed with 25 cycles for MafK and 20 cycles for GAPDH. B, RT reactions were subjected to quantitative realtime PCR.…”

Section: Ngf Rapidly Elevates Mafk Expressionsupporting

confidence: 91%

The Basic Region and Leucine Zipper Transcription Factor MafK Is a New Nerve Growth Factor-Responsive Immediate Early Gene That Regulates Neurite Outgrowth

2002

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We used serial analysis of gene expression to identify new NGF-responsive immediate early genes (IEGs) with potential roles in neuronal differentiation. Among those identified was MafK, a small Maf family basic region and leucine zipper transcriptional repressor and coactivator expressed in immature neurons. NGF treatment elevates the levels of both MafK transcripts and protein. In contrast, there is no effect on expression of the closely related MafG. Unlike many other NGF-responsive IEGs, MafK regulation shows selectivity and is unresponsive to epidermal growth factor, depolarization, or cAMP derivatives. Inhibitor studies indicate that NGF-promoted MafK regulation is mediated by an atypical isoform of PKC but not by mitogenactivated kinase kinase, phospholipase C␥, or phosphoinositide 3Ј-kinase. Interference with MafK expression or activity by small interfering RNA and dominant negative strategies, respectively, suppresses NGF-promoted outgrowth and maintenance of neurites by PC12 cells and neurite outgrowth by immature telencephalic neurons. Our findings support a role for MafK as a novel regulator of neuronal differentiation.

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Section: Ngf Rapidly Elevates Mafk Expressionsupporting

confidence: 91%

The Basic Region and Leucine Zipper Transcription Factor MafK Is a New Nerve Growth Factor-Responsive Immediate Early Gene That Regulates Neurite Outgrowth

2002

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“…Taken together, it seems clear that all three small maf genes utilize multiple first exons. MafG does not appear to have tissue-specific first exons, such as the mafK I N exon that is employed exclusively in neurons (27). Nonetheless, expression analysis revealed that mafG first exons are utilized differentially in various tissues.…”

Section: Discussionmentioning

confidence: 95%

Nrf2 Transcriptionally Activates the mafG Gene through an Antioxidant Response Element

Katsuoka

Motohashi

Engel

et al. 2005

Journal of Biological Chemistry

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109

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Nrf2 accumulates in nuclei upon exposure to oxidative stress, heterodimerizes with a small Maf protein, and activates the transcription of stress target genes through antioxidant response elements (AREs). We found that diethyl maleate (DEM), a well known activator of Nrf2, induces one of the small Maf genes, mafG. To elucidate roles MafG might play in the oxidative stress response, we examined transcriptional regulation of the mouse mafG gene. MafG utilizes three independent first exons that are each spliced to second and third coding exons. Among the small maf genes, mafG showed the strongest response to DEM, and of the three first exons, the highest -fold induction was seen with the proximal first exon (Ic). Importantly, one ARE (Ic-ARE) is conserved in the promoter flanking exon Ic of the human and mouse mafG genes. The Nrf2/MafG heterodimer bound the Ic-ARE and activated transcription, whereas DEM failed to activate mafG in nrf2-null mutant cells. Chromatin immunoprecipitation further revealed that both Nrf2 and small Maf proteins associate with the Ic-ARE in vivo. These results demonstrate that mafG is itself an ARE-dependent gene that is regulated by an Nrf2/small Maf heterodimer and suggest the presence of an autoregulatory feedback pathway for mafG transcriptional regulation.

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“…2) (Motohashi et al, 1996; Motohashi et al, 1998). In the 3’ region of MafK , there is an enhancer referred to as H ematopoietic and C ardiac E nhancer for Maf K (HCEK), which drives MafK expression in both hematopoietic and cardiac tissues (Katsuoka et al, 2000).…”

Section: Gene Structure and Expression Profile Of Smafsmentioning

confidence: 99%

Small Maf proteins (MafF, MafG, MafK): History, structure and function

Katsuoka

Yamamoto

2016

Gene

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204

152

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The small Maf proteins (sMafs) are basic region leucine zipper (bZIP)-type transcription factors. The basic region of the Maf family is unique among the bZIP factors, and it contributes to the distinct DNA-binding mode of this class of proteins. MafF, MafG and MafK are the three vertebrate sMafs, and no functional differences have been observed among them in terms of their bZIP structures. sMafs form homodimers by themselves, and they form heterodimers with cap ‘n’ collar (CNC) proteins (p45 NF-E2, Nrf1, Nrf2, and Nrf3) and also with Bach proteins (Bach1 and Bach2). Because CNC and Bach proteins cannot bind to DNA as monomers, sMafs are indispensable partners that are required by CNC and Bach proteins to exert their functions. sMafs lack the transcriptional activation domain; hence, their homodimers act as transcriptional repressors. In contrast, sMafs participate in transcriptional activation or repression depending on their heterodimeric partner molecules and context. Mouse genetic analyses have revealed that various biological pathways are under the regulation of CNC-sMaf heterodimers. In this review, we summarize the history and current progress of sMaf studies in relation to their partners.

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A core region of the mafK gene I_N promoter directs neurone‐specific transcription in vivo

Cited by 12 publications

References 58 publications

The Basic Region and Leucine Zipper Transcription Factor MafK Is a New Nerve Growth Factor-Responsive Immediate Early Gene That Regulates Neurite Outgrowth

The Basic Region and Leucine Zipper Transcription Factor MafK Is a New Nerve Growth Factor-Responsive Immediate Early Gene That Regulates Neurite Outgrowth

Nrf2 Transcriptionally Activates the mafG Gene through an Antioxidant Response Element

Small Maf proteins (MafF, MafG, MafK): History, structure and function

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