A correlated study on spinal ganglion cells and associated nerve fibers with the light and electron microscopes

Beams, H. W.; Breemen, V. L. van; Newfang, Dorothy M.; Evans, Titus C.

doi:10.1002/cne.900960204

Cited by 44 publications

(10 citation statements)

References 29 publications

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“…The pattern and distribution of Nissl substance, Golgi complex, neurofilaments and dense bodies mostly resemble their counterparts described by earlier workers in spinal and other ganglion cells (BEAMS et al, 1952;DAWSON, HOSSACK and WYBURN, 1955;TENNYSON, 1963;DIXON, 1963), and provide no special features to be discussed here.…”

Section: Discussionsupporting

confidence: 79%

“…Several studies have been made on the fine structure of spinal ganglia in various mammals (BEAMS et al, 1952;DAWSON, HOSSACK and WYBURN, 1955;PALAY and PALADE, 1955;DUNCAN and NAIL, 1960;HIRAOKA and BREEMEN, 1963;TENNYSON, 1964;BUNGE et al, 1967;PANNESE, 1969) but there is no reference to the ultrastructure of the spinal ganglion cells of the slow loris (Nycticebus coucang coucang), a prosimian primate.…”

mentioning

confidence: 99%

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Fine Structure of Neurons in the Spinal Ganglion of Slow Loris (<i>Nycticebus coucang coucang</i>)

Ahmed

1973

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 99%

Fine Structure of Neurons in the Spinal Ganglion of Slow Loris (<i>Nycticebus coucang coucang</i>)

Ahmed

1973

Arch. Histol. Jap., Arch. Histol. Jpn

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“…As early as 1886, histologists observed that the relative quantity of these pigment granules in nerve cells appeared grossly correlated with the age of human and animal subjects (24). These early morphologic observations were subsequently confirmed by numerous other studies (2,8,37). Since it seems reasonable to assume that any extensive intracellular accumulation of these pigment bodies with age may result in significant changes in cellular physiology, several theories of aging have proposed that the deterioration and loss of non-dividing cells may represent one of the most fundamental aspects of biologic aging (4, I0, 29).…”

supporting

confidence: 76%

The Fine Structure of Lipofuscin Age Pigment in the Nervous System of Aged Mice

Samorajski

Ordy

Keefe

1965

The Journal of Cell Biology

172

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An examination of the topographic distribution of lipofuscin pigment granules with the light and electron microscope revcalcd either smaller and randomly "dispersed" or largcr and morc complex "clustered" pigment configurations in the cytoplasm of neurons in the dorsal ganglia and ventral spinal cord of 24-month old male mice. Qualitative comparisons revealcd no major diffcrenccs in shapc, size, complexity, dcnsity, orientation, and cytologic distribution of the pigment bodies in motor and sensory neurons. In gcncral, whcn the pigment granulcs werc quite numerous within the 2 types of cclls, they were smaller in size (~I/~), had a dense homogeneous matrix with few bands or lamcllae, and werc uniformly distributed throughout the cytoplasm. In contrast, when the pigment configurations were less in number, they were usually larger in size (~3/z), had a more complex internal banded structure, and appeared more localized within the cell. Examination of the bands revealed a repeating pattern of ~70 A. The bands appeared to fuse, forming hexagonal arrays of linear densities intersecting at an angle of approximately 120 ° in some regions of the pigment bodies. Structural similarities suggested that the striated membranous bands may be composed of phospholipids.

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“…Evidence derived from studies on the cytochemistry (8), ultracentrifugation (4), and phase-contrast microscopy (17) of nerve cells supports the view that Nissl bodies are real, an opinion which has been supported, for the most part, by electron microscope studies. Beams et al (6) observed in electron micrographs of nerve cells groups of dense, oriented fibrils which they compared to the filaments of the basophilic substance observed in hepatic cells by Dalton et al (16). In all probability these represent poorly preserved endoplasmic reticulum of the Nissl bodies.…”

Section: Discussionmentioning

confidence: 97%

Studies on the Fine Structure of Ultracentrifuged Spinal Ganglion Cells

Beams

Tahmisian

Anderson

et al. 1960

The Journal of Cell Biology

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The following structures were observed in electron micrographs of the mouse spinal ganglion cells: Nissl bodies composed of both aggregated rough-type, largely oriented, membranes of the endoplasmic retieulum and discrete particles; short rodlike mitochondria with well-developed transverse, obliquely or longitudinally arranged eristae, and a relatively typical Golgi complex. The components of ultracentrifuged ganglion cells (400,000 times gravity for 20 minutes) are stratified, the layers appearing in the order of their decreasing density as follows: (1) A microsomal or ergastoplasmic layer which may be further divided into three sublayers without sharp boundaries, namely, a discrete particle layer, a layer of discrete particles and highly distorted membranes of the endoplasmic reticulum, and a layer composed of relatively intact, but stretched membranes of the endoplasmic reticulum and discrete particles. (2) Mitochondria constitute a relatively broad layer. They are sometimes stretched; however, they retain most of their fine structure. The stratified nucleus is found within the mitochondrial layer. (3) A relatively wide layer of tightly packed vesicles. (4) At the centripetal end, resting against the cell membrane, are a few lipid vacuoles. A comparison is made between the ultrastructure of the stratified layers in situ and those described by others in differentially uhracentrifuged homogenates.Experiments utilizing sufficiently high centrifugal force to cause displacement and stratification of the microscopically visible contents of cells (eggs) were first made by Gurwitsch (21). This study and those which followed (cf. 39) demonstrated that the displaced components and inclusions were stratified in the order of their relative densities, a condition which did not markedly injure the egg or interfere with its normal development. The experiments of Harvey (26) further demonstrated that stratified Arbacia eggs may be pulled into nearly equal halves and the halves into quarters, each of which when fertilized develop into normal plutei, indicating that none of the formed elements is essential to early development in this form (27). In addition, the centrifuge has been used in the study of "specific organ forming substance," "surface active substance," polarity and bilaterality, viscosity, and the distribution of enzymes (cf. 3, 27, 29, 32). The experiments on the distribution of enzymes in cells, utilizing both centrifugation and histochemistry, were not sufficiently specific, except in a relatively few instances, to link given enzymes or their precursors to specific morphological components Although these studies were limited to cells of relatively

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A correlated study on spinal ganglion cells and associated nerve fibers with the light and electron microscopes

Cited by 44 publications

References 29 publications

Fine Structure of Neurons in the Spinal Ganglion of Slow Loris (<i>Nycticebus coucang coucang</i>)

Fine Structure of Neurons in the Spinal Ganglion of Slow Loris (<i>Nycticebus coucang coucang</i>)

The Fine Structure of Lipofuscin Age Pigment in the Nervous System of Aged Mice

Studies on the Fine Structure of Ultracentrifuged Spinal Ganglion Cells

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