A cost-effective and sensitive TLC-densitometric identification of meloxicam

Abstract

The influence of different chromatographic conditions and the process of spot visualization on determining the limit of detection as well as quantification (LOD and LOQ) of meloxicam by TLC-densitometric technique was estimated. Of all chromatographic conditions tested, the lowest limiting values, thus the best sensitivity, in the NP-TLC system was achieved on silica gel 60F254 and neutral aluminum oxide plates developed with the mobile phase consisting of ethyl acetate/toluene/n-butylamine (2:2:1, V/V/V). In the case of the RP-TLC method, a mixture of methanol/water (8:2, V/V) enabled densitometric detection of meloxicam at the lowest concentration level on RP-8F254 and RP-18F254 plates. Additionally, the smallest LOD value of meloxicam ensured crystalline violet and gentian violet as visualization agents on silica gel 60F254 and neutral aluminum oxide 150F254 plates, resp. Comparison of the densitometrically obtained spectra of meloxicam drug and its standard after the use of appropriate visualization agents could be a good and cheap alternative tool for the identification of meloxicam as an active pharmaceutical ingredient.

A 20-Metal Zn(II)–Cd(II)–Eu(III) Nanocluster with Qualitative and Quantitative Luminescence Detection of Meloxicam (an Anti-Inflammatory Drug)

“…As shown in Table a, the recoveries and relative standard deviations (RSDs) are in the ranges of 96.85–104.64% and 0.84–5.21%, respectively. The detection limit (3σ/ k , σ is the standard deviation) of MLX is found to be 2.31 × 10 –2 nM, which is lower than most of those reported by the traditional analysis methods, ,− suggesting the high sensitivity of 1 to MLX. The luminescence change of 1 caused by the addition of MLX is fast, with a response time of about 5 s (Figure ).…”

“…Meloxicam (MLX), chemically named [4-hydroxy-2-methyl- N -(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-carboxamide-1,1-dioxide] (Scheme a), is a novel nonsteroidal anti-inflammatory drug (NSAID) with analgesic and antipyretic properties and has been widely applied to relieve symptoms of inflammation and pain. − MLX is stable in water for more than 7 days at room temperature, but on the other hand, it has become one of the common microcontaminants in surface waters and sewage. , Some traditional analysis methods, such as high-performance liquid chromatography (HPLC), , thin-layer chromatography (TLC), spectrophotometric, and electrophoresis, have been developed for the determination of MLX. For example, Alina et al first reported the use of visualization agents to detect MLX on a thin layer recently . These methods have characteristics of high selectivity and good precision.…”

“…For example, Alina et al first reported the use of visualization agents to detect MLX on a thin layer recently. 11 These methods have characteristics of high selectivity and good precision. However, their pretreatment processes are complicated, and they usually need long turnaround times, which makes them difficult to be widely used in fast control analyses.…”