A cost-effective carbohydrate fermentation test for yeast using microtitre plate

Kali, Arunava; Srirangaraj, Sreenivasan; Charles, Pravin

doi:10.4103/0255-0857.154884

Cited by 13 publications

(10 citation statements)

References 4 publications

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“…After the oral administration of probiotic organism, it is subjected to stressing conditions from the host which begins in the stomach with an acid pH between 1.5 and 3 and in the upper intestine and colon where high concentration of bile is encountered. It is, therefore, necessary that an efficient probiotic is capable of growing in an acidic environment and high concentration of bile [ 17 ]. As it was shown in this study.…”

Section: Discussionmentioning

confidence: 99%

Probiotic characterization of lactic acid bacteria isolated from swine intestine

Balasingham¹,

Valli²,

Radhakrishnan³

et al. 2017

Vet World

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Aim:A study was conducted with the objective to isolate probiotic microorganisms from swine intestine.Materials and Methods:In this study 63 isolates (24 caeca, 24 colon mucosal scrapings, and 15 rectal swab samples) were collected from Large White Yorkshire pigs. The isolates were inoculated and grown in de Man Rogosa Sharpe broth at 37°C with 5% CO2 for 48 h and subjected to morphological identification. Colonies having Gram-positive rods were selected for further physiological and biochemical identification tests, which were conducted in triplicate in two runs for each of the selected isolates using a standard protocol. Probiotic properties among the identified species were determined through the implementation of several tests related with pH tolerance, bile tolerance, and antimicrobial activity.Results:Morphological identification revealed that only 23 isolates were Gram-positive rods. Physiological tests performed on these 23 isolates further revealed that four of them did not exhibit any growth, at all conditions studied. The rest 19 isolates were, therefore, selected and subjected to biochemical tests. Six isolates were rejected because they were oxidase and nitrate reduction positive. From the 13 isolates subjected to sugar fermentation tests, speciation of only two isolates could be ascertained, one of the isolates showed characteristics for Lactobacillus acidophilus and the other for Lactobacillus plantarum. These two isolates were assessed for the strain possessing maximum probiotic property, and it was inferred that both – L. plantarum and L. acidophilus could tolerate a wide pH range (2-9), a wide bile concentration (0.05-0.3%) and revealed antimicrobial activity toward Escherichia coli, and Enterobacter spp.Conclusion:L. plantarum and L. acidophilus were isolated from swine intestine and were found to have good probiotic properties.

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Section: Discussionmentioning

confidence: 99%

Probiotic characterization of lactic acid bacteria isolated from swine intestine

Balasingham¹,

Valli²,

Radhakrishnan³

et al. 2017

Vet World

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“…The growing Candida specimen were identified using cornmeal agar and tween 80 that were incubated at 42-45°C 12,13 . We also conducted carbohydrate test to identify Candida species 14 . Furthermore, we conducted resistance test using disc diffusion method on Mueller Hinton agar with 2% glucose and methylen blue.…”

Section: Methodsmentioning

confidence: 99%

Nystatin Profile on Candida Species in HIV/AIDS Patients with Oral Candidiasis: A Phenomenology Study

Murtiastutik¹,

Maharani²,

Rahmadewi³

et al. 2019

J Pure Appl Microbiol

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“…Candida species were identified preliminary according to the colors produced by the colonies on CHROMagar Candida [17] The following tests for phenotypic differentiation of green isolates were carried out: Germ tube formation test in plasma [18], chlamydospores production in milk agar, macro and micro morphology in tobacco agar and growth at 45 °C according to Pineda et al [19]. Conventional yeast fermentation test was also carried out [20,21] .…”

Section: Methodsmentioning

confidence: 99%

Vaginal colonization and vulvovaginitis by Candida species in pregnant women from Northern of Colombia

Álvarez

Trujillo

Hoyos

et al. 2018

archmed

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Objective: identify the vaginal colonizing Candida species and VVC species, predisposing factors and susceptibility against fluconazole in pregnant women attending gynecological outpatient of a maternal clinic in Cartagena (Colombia). Vulvovaginal candidiasis (VVC) is a common infection that affects a large proportion of women of childbearing age. It is estimated that about 75% of healthy women have had at least one episode of VVC in her life. Vaginal colonization by Candida spp. can vary from 10 to 17% and this is increased by 35% during pregnancy. The epidemiological behavior of Candida in VVC tends to be variable and this will depend on the level of vaginal estrogens, conditions of immunosuppression or chronicity of the process. Materials and Methods: the laboratory study included the direct examination of vaginal swabs, by fresh and differential staining of smears and cultures. The isolated species were identified by phenotypic tests and by multiplex PCR and CLSI M27-A3 microdilution method was used to evaluate susceptibility to fluconazole. Results: the frequent clinical diagnosis of bacterial vaginosis, did not correspond with the laboratory diagnosis in which predominated the intermediate biota. The results showed Candida albicans as the most common isolated species, followed by Candida tropicalis and Candida krusei. Candida colonization occurred in 71.4% and 28.6% of the population was diagnosed with vaginal candidiasis. All isolates were susceptible to fluconazole. Conclusion: The most common factors for colonization were related to clothing and for vaginal candidiasis to the use of vaginal lubricants.

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A cost-effective carbohydrate fermentation test for yeast using microtitre plate

Cited by 13 publications

References 4 publications

Probiotic characterization of lactic acid bacteria isolated from swine intestine

Probiotic characterization of lactic acid bacteria isolated from swine intestine

Nystatin Profile on Candida Species in HIV/AIDS Patients with Oral Candidiasis: A Phenomenology Study

Vaginal colonization and vulvovaginitis by Candida species in pregnant women from Northern of Colombia

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