A Cost-Effective Nano-Sized Curcumin Delivery System with High Drug Loading Capacity Prepared via Flash Nanoprecipitation

Chen, Zhuo; Fu, Zhinan; Li, Li; Ma, Enguang; Guo, Xuhong

doi:10.3390/nano11030734

Cited by 10 publications

(4 citation statements)

References 40 publications

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“…A higher total flow rate (50 mL/min), representing a faster mixing, decreased the CUR encapsulation efficiency from 49% to 17% and loading efficiency from 11% to 5% compared with a lower total flow rate (10 mL/min). The reason is that the encapsulation/loading of CUR relies on a flash nanoprecipitation where the hydrophobic CUR was dissolved in the water-miscible 2-MI methanol solution and rapidly mixed with antisolvent (ZIN water solution) so that the nanoprecipitation happened and the precipitated CUR could be encapsulated by the synthesised ZIF-8 nanoparticles at the same time [ 80 ]. Therefore, an over-high total flow rate (higher than 50 mL/min) resulted in a very short mixing time (less than 0.7 ms), which was not enough for sufficient nanoprecipitation of CUR, leading to a relative low CUR encapsulation efficiency.…”

Section: Resultsmentioning

confidence: 99%

Microfluidic-Assisted ZIF-Silk-Polydopamine Nanoparticles as Promising Drug Carriers for Breast Cancer Therapy

Gao,

Mansor,

Winder

et al. 2023

Pharmaceutics

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Metal–organic frameworks (MOFs) are heralded as potential nanoplatforms for biomedical applications. Zeolitic imidazolate framework-8 (ZIF-8), as one of the most well known MOFs, has been widely applied as a drug delivery carrier for cancer therapy. However, the application of ZIF-8 nanoparticles as a therapeutic agent has been hindered by the challenge of how to control the release behaviour of anti-cancer zinc ions to cancer cells. In this paper, we designed microfluidic-assisted core-shell ZIF-8 nanoparticles modified with silk fibroin (SF) and polydopamine (PDA) for sustained release of zinc ions and curcumin (CUR) and tested these in vitro in various human breast cancer cells. We report that microfluidic rapid mixing is an efficient method to precisely control the proportion of ZIF-8, SF, PDA, and CUR in the nanoparticles by simply adjusting total flow rates (from 1 to 50 mL/min) and flow rate ratios. Owing to sufficient and rapid mixing during microfluidic-assisted nanoprecipitation, our designer CUR@ZIF-SF-PDA nanoparticles had a desired particle size of 170 nm with a narrow size distribution (PDI: 0.08), which is much smaller than nanoparticles produced using traditional magnetic stirrer mixing method (over 1000 nm). Moreover, a properly coated SF layer successfully enhanced the capability of ZIF-8 as a reservoir of zinc ions. Meanwhile, the self-etching reaction between ZIF-8 and PDA naturally induced a pH-responsive release of zinc ions and CUR to a therapeutic level in the MDA-MB-231, SK-BR-3, and MCF-7 breast cancer cell lines, resulting in a high cellular uptake efficiency, cytotoxicity, and cell cycle arrest. More importantly, the high biocompatibility of designed CUR@ZIF-SF-PDA nanoparticles remained low in cytotoxicity on AD-293 non-cancer cells. We demonstrate the potential of prepared CUR@ZIF-SF-PDA nanoparticles as promising carriers for the controlled release of CUR and zinc ions in breast cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Microfluidic-Assisted ZIF-Silk-Polydopamine Nanoparticles as Promising Drug Carriers for Breast Cancer Therapy

Gao,

Mansor,

Winder

et al. 2023

Pharmaceutics

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“…As shown in Figure 7 , the sizes of Cur-loaded CS nanocomplex decreased from 400 nm to 190 nm when the Re ranged from 199 to 2385. It was observed that the particle size did not further decrease when the Re exceeded 1590, which was owing to the homogenous mixing in the FNC process when Re reaches a certain degree [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

Scalable Manufacture of Curcumin-Loaded Chitosan Nanocomplex for pH-Responsive Delivery by Coordination-Driven Flash Nanocomplexation

Xia

et al. 2022

Polymers

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Metal coordination-driven nanocomplexes are known to be responsive to physiologically relevant stimuli such as pH, redox, temperature or light, making them well-suited for antitumor drug delivery. The ever-growing demand for such nanocomplexes necessitates the design of a scalable approach for their production. In this study, we demonstrate a novel coordination self-assembly strategy, termed flash nanocomplexation (FNC), which is rapid and efficient for the fabrication of drug-loaded nanoparticles (NPs) in a continuous manner. Based on this strategy, biocompatible chitosan (CS) and Cu2+ can be regarded anchors to moor the antitumor drug (curcumin, Cur) through coordination, resulting in curcumin-loaded chitosan nanocomplex (Cur-loaded CS nanocomplex) with a narrow size distribution (PDI < 0.124) and high drug loading (up to 41.75%). Owing to the excellent stability of Cur-loaded CS nanocomplex at neutral conditions (>50 days), premature Cur leakage was limited to lower than 1.5%, and pH-responsive drug release behavior was realized in acidic tumor microenvironments. An upscaled manufacture of Cur-loaded CS nanocomplex is demonstrated with continuous FNC, which shows an unprecedented method toward practical applications of nanomedicine for tumor therapy. Furthermore, intracellular uptake study and cytotoxicity experiments toward H1299 cells demonstrates the satisfied anticancer efficacy of the Cur-loaded CS nanocomplex. These results confirm that coordination-driven FNC is an effective method that enables the rapid and scalable fabrication of antitumor drugs.

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“…CUR-loaded zein NPs solution (1 ml) was placed into a dialysis bag (MWCO 10 kDa) and immersed in PBS solution (19 ml) containing 1% sodium dodecyl sulfonate (SDS, w/v) at pH 7.4 or 5.0 and 37 C. 17,18 The drug release process was studied over at a specific time interval.…”

Section: In Vitro Release (Surfactant-assisted)mentioning

confidence: 99%

Controlled fabrication of drug‐loaded protein nanoparticles via flash nanoprecipitation

Zhao

Wang

et al. 2022

AIChE Journal

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Protein nanoparticles (NPs) are perspective vehicles for delivery of therapeutic agents. However, whether they will ever reach the stage of widespread application relies crucially on the development of reliable fabrication strategies and techniques.Herein, we report a continuous precipitation approach, namely flash nanoprecipitation (FNP) for efficient construction of drug-loaded protein NPs with regulated size and properties. Our design relies on rapid mixing of solvent streams containing drug

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A Cost-Effective Nano-Sized Curcumin Delivery System with High Drug Loading Capacity Prepared via Flash Nanoprecipitation

Cited by 10 publications

References 40 publications

Microfluidic-Assisted ZIF-Silk-Polydopamine Nanoparticles as Promising Drug Carriers for Breast Cancer Therapy

Microfluidic-Assisted ZIF-Silk-Polydopamine Nanoparticles as Promising Drug Carriers for Breast Cancer Therapy

Scalable Manufacture of Curcumin-Loaded Chitosan Nanocomplex for pH-Responsive Delivery by Coordination-Driven Flash Nanocomplexation

Controlled fabrication of drug‐loaded protein nanoparticles via flash nanoprecipitation

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