DDS can also be in the form of rapid parenteral intravenous, intramuscular, or subcutaneous delivery, which is suitable for emergency situations and mostly accompanied with pain, and hence not preferred by patients. [2,3] Inhalation drug delivery is a painless DDS method received through the lungs that is mainly used for respiratory diseases with the risk of overdosing upon self-administrations. [4] Another method of drug delivery is transdermal drug delivery through skin layers via application of creams, hypodermic needles, ultrasound, electroporation, iontophoresis, or use of microneedles (MNs). [4][5][6] In therapeutic monitoring, early stage diagnostics, efficiency of biological samples, timely analysis, non-invasiveness, and potential for self-administration are all highly desirable. [7] Point-of-care (POC) medical tests using MNs have the potential to provide rapid, minimally invasive, and real time diagnostics, [7] sampling either capillary blood or interstitial fluid (ISF) in competition with venous blood, breath, stool, and sputum. ISF has been shown to contain 83% of serum proteins, only 50% of which are found in serum, indicting the significance of ISF biomarkers for monitoring and diagnostics. [8,9] Therefore, ongoing pathophysiological events such as chronic conditions can be rapidly and painlessly detected using POC MN diagnostic systems enabling collection of ISF samples with unique biomarkers using MN systems. [10] Single MNs can be used as a painless alternative to steel hypodermic needles but they are more likely to be used as arrays of multiple MNs incorporated into diagnostic or therapeutic patches. [11] Their painless, non-invasive, and selfadministrative capabilities have received increasing attention in recent years. [12] Conventional hypodermic needles can induce pain, are semi-invasive, create hazardous wastes, and require trained medical staff for administration. Instead, minimally invasive MNs can be administrated without discomfort to the patient by bypassing the stratum corneum (SC) barrier of skin, [12][13][14] while avoiding contact with nerve system and blood vessels. It can be self-administrated and create less waste in comparison to the traditional hypodermic needles. [15][16][17] MNs are potentially capable of delivering ionic, hydrophilic, and large molecular weight drugs more effectively than passive diffusion using needle-free patches or topical creams. [18,19] Thus, MNs are an attractive candidate for delivery of drugs including insulins, growth hormones, proteins, siRNA, immunobiological,