Research was conducted to indicate the impact of the increased flow of thermal insulation materials on the environment due to the implementation of the new regulations on energy efficiency of buildings. The regulations on energy efficiency of buildings in Serbia came into force on 30 September 2012 for all new buildings as well as for buildings in the process of rehabilitation and reconstruction. For that purpose, the carbon footprint was analyzed in three scenarios (BS, S1 and S2) for which the quantities of construction materials and processes were calculated. The life cycle analysis (LCA), which is the basis for analyzing the carbon life cycle (LCACO 2 ), was used in this study. Carbon Calculator was used for measuring carbon footprint, and URSA program to calculate the operational energy. This study was done in two phases. In Phase 1, the embodied carbon was measured to evaluate short-term effects of the implementation of the new regulations. Phase 2 included the first 10 years of building exploitation to evaluate the long-term effects of the new regulations. The analysis was done for the period of 10 years, further adjustments to the regulations regarding energy efficiency of the buildings in Serbia are expected in accordance with EU directives. The study shows that, in the short-run, Scenario BS has the lowest embodied carbon. In the long-run, after 3.66 years, Scenario S2 becomes a better option regarding the impact on the environment. The study reveals the necessity to include embodied carbon together with the whole life carbon to estimation the impact of a building on the environment. emission by 26.90% by 2020 was identified by Kim et al. [3]. The studies, often quoted as examples of the impact on the environment from the construction sector [4], show that the use of cement is responsible for 8.6% of CO 2 emission in the world [5]. Cement is widely used in civil engineering, both to produce concrete and as matrix for many other products which are used on global level. One of the basic concepts of the economics of natural resources and environment is sustainability and sustainable development [6], especially sustainability of transportation and logistics [7][8][9][10][11]. In relation to sustainability, EU commission [12] decided that it is necessary for the civil engineering sector to implement measures to reduce emissions and mitigate climate changes. The measures refer to the savings in using primary materials, using recycled materials, reducing waste quantity, returning materials into energy cycles, and reducing water and energy consumption. This also includes the studies on possible savings in energy consumption in buildings [13][14][15][16]. According to Vourdobas [17], energy consumption in buildings accounts for 40% of total energy consumption in EU.Recommendations about the ways to reduce energy consumption and implement the measures for energy efficiency of the buildings are given in the approved EU Directive (Directive2002/91/EC and Directive 2010/31/EU) [18,19]. Serbia's legislations are tryi...