A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification

Chakraborty, Samarshi; Ji, HaYeun; Kabadi, Ami M.; Gersbach, Charles A.; Christoforou, Nicolas; Leong, Kam W.

doi:10.1016/j.stemcr.2014.09.013

Cited by 186 publications

(168 citation statements)

References 21 publications

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“…A variety of second-generation dCas9-activator fusions have been subsequently engineered that incorporate varying copies of VP16 Chakraborty et al, 2014), the tripartite activator VPR (VP64-p65-Rta, where Rta is the transcriptional activation domain of the Epstein-Barr virus) , or the repeating peptide array SunTag that subsequently recruits multiple copies of an antibody-VP64 fusion (Tanenbaum et al, 2014) (Figure 4a). These various constructs offer strong gene activation in a variety of mammalian cell types (Chavez et al, 2016).…”

Section: Epigenome Editingmentioning

confidence: 99%

CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

Komor¹,

Badran²,

Liu³

2017

Cell

270

214

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The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this review we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and some of the remarkable advancements in basic research, biotechnology, and therapeutics development that these developments have facilitated.

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Section: Epigenome Editingmentioning

confidence: 99%

CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

Komor¹,

Badran²,

Liu³

2017

Cell

270

214

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“…Facilitated by many of the insights gained from zinc-finger transcription factor technology, both TALEs and CRISPR-Cas9 have now further expanded the possibilities of engineered transcriptional activators and repressors. For example, TALEs and CRISPR-Cas9 have enabled rapid construction of custom genetic circuits and logic gates (Gaber et al 2014;Lebar et al 2014;Liu et al 2014b), complex gene regulation networks (Nielsen and Voigt 2014;Nissim et al 2014), and even facilitated cellular reprogramming (Gao et al 2013) and the differentiation of mouse embryonic fibroblasts to skeletal myocytes (Chakraborty et al 2014). dCas9 transcriptional effectors have even been used to efficiently mediate repression and activation of endogenous genes in Drosophila (Lin et al 2015b) and in plant cells (Piatek et al 2015).…”

Section: Applications Of Targeted Transcriptional Regulationmentioning

confidence: 99%

Genome-Editing Technologies: Principles and Applications

Gaj

Sirk

Shui

et al. 2016

Cold Spring Harb Perspect Biol

297

142

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Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy. Here we review three foundational technologies-clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). We discuss the engineering advances that facilitated their development and highlight several achievements in genome engineering that were made possible by these tools. We also consider artificial transcription factors, illustrating how this technology can complement targeted nucleases for synthetic biology and gene therapy.

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“…34 Remarkably, these activators proved to be orthogonal to each other since they activated only the promoter they targeted i.e., they did not show any crosstalk. Improvements have been achieved by fusing to dSpCas9 either 2 copies of VP64 (VP64-dSpCas9-VP64) 35 or a hybrid tripartite activation domain made of VP64, p65 and the Rta activation domains (hence named VPR).…”

Section: ããmentioning

confidence: 99%

“…However, it still required that each gene was targeted by 3 or 4 gRNAs, whereas Chakraborty et al 35 pointed out that at least one human locus (Myod1) was clearly activated by a single guide RNA in conjunction with VP64-dSpCas9-VP64.…”

Section: ããmentioning

confidence: 99%

CRISPR-Cas type II-based Synthetic Biology applications in eukaryotic cells

Marchisio

Huang

2017

RNA Biology

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The CRISPR-Cas system has rapidly reached a huge popularity as a new, powerful method for precise DNA editing and genome reengineering. In Synthetic Biology, the CRISPR-Cas type II system has inspired the construction of a novel class of RNA-based transcription factors. In their simplest form, they are made of a CRISPR RNA molecule, which targets a promoter sequence, and a deficient Cas9 (i.e. deprived of any nuclease activity) that has been fused to an activation or a repression domain. Up-and downregulation of single genes in mammalian and yeast cells have been achieved with satisfactory results. Moreover, the construction of CRISPR-based transcription factors is much simpler than the assembly of synthetic proteins such as the Transcription Activator-Like effectors. However, the feasibility of complex synthetic networks fully based on the CRISPR-dCas9 technology has still to be proved and new designs, which take into account different CRISPR types, shall be investigated.

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A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification

Cited by 186 publications

References 21 publications

CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

Genome-Editing Technologies: Principles and Applications

CRISPR-Cas type II-based Synthetic Biology applications in eukaryotic cells

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