A critical review on submerged production of mushroom and their bioactive metabolites

Dudekula, Umera Tasleem; Doriya, Kruthi; Devarai, Santhosh Kumar

doi:10.1007/s13205-020-02333-y

Cited by 34 publications

(16 citation statements)

References 70 publications

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“…According to Zhang et al, the antitumor activity of the mushroom was reported firstly by Lucas by discovering the inhibitory effect of a substance from Boletus edulis against Sarcoma S-180 tumor cells which is also the first report for bioactivity of Basidiomycetes mushrooms [14,15]. Because of their preventive effect on oncogenesis and tumor metastasis and stimulating effect on the host immune system, mushroom polysaccharides are widely used especially in Asian countries with chemotherapy [5,12]. Other than antimicrobial [16], antioxidant [17] antiviral [18], antiallergic [19], anti-inflammatory [5], antidiabetic [20], hepatoprotective [21], and radioprotective [22] activities and immunological activities of polysaccharides derived from mushrooms are well-documented [23,24,25] and most extensively studied 5 .…”

Section: Discussionmentioning

confidence: 99%

“…Collecting or producing the fruit bodies is a season-depending, time-consuming, and/or laborintensive process. On the other hand, a submerged culture of mushrooms can be a promising alternative for easy, fast, and efficient production of bioactive metabolites [12]. In this view of point, the anticancer activities of exopolysaccharides (EPS) from submerged culture of six mushroom species, Coprinus comatus, Fistulina hepatica, Panus neostrigosus, Laetiporus sulphureus, Polyporus squamosus, and Lenzites betulinus, were investigated on the rat glioma (C6) cells in the present study.…”

Section: Introductionmentioning

confidence: 97%

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Determination of Antiproliferative Effects of Exopolysaccharides from Six Mushroom Species on Glioma Cells

CAKA¹,

Vatan

Kabadere

et al. 2022

Cumhuriyet Science Journal

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Glial tumors are the largest and danger group of central nervous system tumors. The use of natural products now has been contemplated of exceptional value in the control of cancer. Mushrooms have been used for many centuries, not just as a food, but also to treat many illnesses. Therefore, we aimed to evaluate the effect of exopolysaccharides (EPS) obtained from six different edible mushrooms on the survival of glioma cells. In this study the effects of 0.4, 1, 2, 4 and 6 μg/mL doses of EPSs from six mushroom species Coprinus comatus, Fistulina hepatica, Panus neostrigosus, Laetiporus sulphureus, Polyporus squamosus, and Lenzites betulinus were investigated on the rat glioma cell line (C6) in two different periods by MTT assay. According to our results 0.4 and 1 μg/mL of EPSs from six mushroom species were not effective or less effective, but 2, 4 and 6 μg/mL doses killed glioma cells about 27 to 71 % for 24 hours, 35 to 78 % for 48 hours As a result, these mushroom EPSs showed different cytotoxicity to glioma cells time and dose-dependently. These findings can be suggested that the anti-tumor effects of EPSs can be potential use in clinical applications to treat glioma. Further studies are needed to understand these effects more clearly on glioma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Determination of Antiproliferative Effects of Exopolysaccharides from Six Mushroom Species on Glioma Cells

CAKA¹,

Vatan

Kabadere

et al. 2022

Cumhuriyet Science Journal

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“…The ensuing fermentation stage, executed within controlled bioreactors or fermentation vessels, demands precise regulation of environmental parameters encompassing temperature, pH, oxygen availability, and agitation. 22 These conditions are meticulously modulated to optimize fungal proliferation and mycoprotein production. During the metabolic progression, the fungal cells actively metabolize the nutrients in the submerged medium, ultimately culminating in the biosynthesis of biomass enriched with mycoprotein.…”

Section: Methods Of Productionmentioning

confidence: 99%

Mycoprotein: production and nutritional aspects: a review

Majumder,

Miatur,

Saha

et al. 2024

Sustainable Food Technol.

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“…On the other hand, it provides several advantages such as higher productivity and yields, lower labor costs, and lower contamination risk. Submerged culture offers the potential advantages of faster production of EPS within a smaller space, reduced possibility of contamination, and better control of cultivation conditions such as carbon source and temperature ( Dudekula et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Production, Extraction, and Solubilization of Exopolysaccharides Using Submerged Cultures of Agaricomycetes

Dávila Giraldo,

Villanueva Baez,

Zambrano Forero

et al. 2023

BIO-PROTOCOL

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Macrofungi, also known as mushrooms, can produce various bioactive compounds, including exopolysaccharides (EPS) with distinct biological properties and subsequent industrial applications in the preparation of cosmetics, pharmaceuticals, and food products. EPS are extracellular polymers with diverse chemical compositions and physical properties secreted by macrofungi in the form of capsules or biofilms into the cellular medium. Submerged cultivation is an industrially implemented biotechnological technique used to produce a wide variety of fungal metabolites, which are of economic and social importance due to their food, pharmaceutical, and agronomic applications. It is a favorable technique for cultivating fungi because it requires little space, minimal labor, and low production costs. Moreover, it allows for control over environmental variables and nutrient supply, essential for the growth of the fungus. Although this technique has been widely applied to yeasts, there is limited knowledge regarding optimal growth conditions for filamentous fungi. Filamentous fungi exhibit different behavior compared to yeast, primarily due to differences in cell morphology, reproductive forms, and the type of aggregates generated during submerged fermentation. Furthermore, various growing conditions can affect the production yield of metabolites, necessitating the development of new knowledge to scale up metabolite production from filamentous fungi. This protocol implements the following culture conditions: an inoculum of three agar discs with mycelium, agitation at 150 rpm, a temperature of 28 °C, an incubation time of 72 h, and a carbon source concentration of 40 g/L. These EPS are precipitated using polar solvents such as water, ethanol, and isopropanol and solubilized using water or alkaline solutions. This protocol details the production procedure of EPS using submerged culture; the conditions and culture medium used are described. A detailed description of the extraction is performed, from neutralization to lyophilization. The concentrations and conditions necessary for solubilization are also described. Key features • Production and extraction of EPS from submerged cultures of mycelial forms of macrofungi. • Modification of the method described by Fariña et al. (2001), extending its application to submerged cultures of mycelial forms of the macrofungi. • Determination of EPS production parameters in submerged cultures of mycelial forms of macrofungi. • EPS solubilization using NaOH (0.1 N). Graphical overview

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A critical review on submerged production of mushroom and their bioactive metabolites

Cited by 34 publications

References 70 publications

Determination of Antiproliferative Effects of Exopolysaccharides from Six Mushroom Species on Glioma Cells

Determination of Antiproliferative Effects of Exopolysaccharides from Six Mushroom Species on Glioma Cells

Mycoprotein: production and nutritional aspects: a review

Production, Extraction, and Solubilization of Exopolysaccharides Using Submerged Cultures of Agaricomycetes

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