A critical study of the histochemical techniques for acid phosphatase, with a description of an azo‐dye method

The Disruption of Lysosome-like Particles of Solanum tuberosum Cells during Infection by Phytophthora erythroseptica Pethybr.

“…The standard coupling azo dye method (Grogg & Pearse, 1952) was done on fresh and fixed sections and tissue culture material with sodium a-naphthyl phosphate as substrate (Koch-Light Laboratories Ltd.) and Fast garnet GBC salt (G. T. Gurr, Ltd.) with incubation for 15 to 60 min. (b) The post-coupling azo dye method (Rutenberg & Seligman, 1955) was used on similar tissues with sodium 6-benzoyl-2-naphthyl phosphate (Koch-Light, Ltd.) and incubation for I hr at 37' followed by coupling with Fast blue B salt (G. T. Gurr, Ltd.) at 0 ' .…”

Histochemical Demonstration of Leucocyte Acid Phosphatase in Health and in Disease

“…The first azo-dye technique for acid phosphatase with a-naphthyl phosphate as substrate was described by Seligman and Manheimer (1948) and later modified by Grogg and Pearse (1952). This method was subsequently applied to the study of leucocyte acid phosphatase by others (Rozenszajn et al, 1963 ;Wulf, 1963).…”

Enzyme Histochemistry on Normal and Pathological Human Thymic Tissues.

“…AcP (orthophosphoric monoester phosphohydrolase, EC 3.1.3.2) [6] catalyzes the hydrolysis of esters of orthophosphoric acid with various alcohol and phenols [7]. To demonstrate AcP activity, tissue sections were fixed in 60% acetone for 2 min followed by incubation in 2.2 mM naphtol AS-BI phosphate as substrate and 3.8 mM Fast Red Violet as capture agent dissolved in 0.1 M acetate buffer pH 5.0.…”