The Disruption of Lysosome-like Particles of Solanum tuberosum Cells during Infection by Phytophthora erythroseptica Pethybr.

Solubilization of Molecular Forms of Lysosomal Acid Phosphatase of Solanum tuberosum L. Leaves during Infection by Phytophthora infestans (Mont.) de Bary

“…Such fragility changes are known to be preceded by an altered permeability of the lysosomal membrane, an event which could also permit the entry of an inhibitor of lysosomal acid phosphatase, perhaps of fungal origin, which would account for the observed decrease in the particulate enzyme location. Although none of these possibilities can be excluded at present, the earlier histochemical observations (Pitt & Coombes, 1968, 1969 have already shown that lysosomal disruption and increased cytoplasmic staining occur during fungal infections of potato tubers.…”

“…and Plij.tophthora infestans (Mont.) de Bary are accompanied by the release of acid hydrolases from a sedimentable to a soluble form (Pitt & Coombes, 1968, 1969. However, the increased lysosomal enzyme activity in the supernatantfluid fractions of tissue extracts may be accompanied by activation of preformed enzyme (Pitt & Galpin, 1973a), and consequently it is not clear if the soluble enzyme has arisen from a previously bound form within the host or parasite or through activation of an already soluble inactive form.…”

Immunochemical Identification of a New Molecular Form of Acid Phosphatase of Host Origin Arising during Infection of Potato Tubers by Phytophthora erythroseptica Pethybr.

“…described by Pitt & Coombes (1968). After this time disease lesions extended through approximately half of the tuber.…”

Release of Hydrolytic Enzymes from Cytoplasmic Particles of Solanum Tuber Tissues During Infection by Tuber-rotting Fungi

“…Although lysosomes have been implicated in a number of cellular processes (de Duve, 1963) particular attention has been directed to their role in animal cell pathology Bernheimer & Schwartz, 1964;Johnson, 1968). Histochemical observations (Pitt & Coombes, 1968) have shown that during infection of Solanum tissues by Phytophthora erythroseptica there was swelling and disruption of lysosome-like host-cell particles, accompanied by increased diffuse cytoplasmic staining for acid phosphatase and esterase. The present work adopts a primarily biochemical approach complemented with relevant histochemical observations to discover whether lysosomal damage occurring as a symptom of pink-rot disease of potato tubers, revealed by predominantly histochemical methods in earlier work (Pitt & Coombes, 1968), is a general feature of infection of potato tubers and callus tissues by other tuber-rotting fungi.…”

“…The isolate Iof Phytophthora erythroseptica Pethybr. was that used earlier (Pitt & Coombes, 1968). Fusarium caeruleum (Lib) Sacc.…”