A critique of prevailing approaches to nutrient risk analysis pertaining to food supplements with specific reference to the European Union

Verkerk, Robert H. J.; Hickey, Stephen

doi:10.1016/j.tox.2009.12.017

Cited by 7 publications

(2 citation statements)

References 48 publications

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“…As a consequence, there are no established methods for determining quantity or nutritional role of dietNAs. However, some recent publications indicate that this omission may soon be repaired [1,2]. Our former research on the role of dietNAs as a food component resulted in the development of a toolbox of methods to enable an initial characterization of the abundance and quality of dietNAs in different raw and processed foods [3].…”

Section: Introductionmentioning

confidence: 99%

Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins

Kościelak,

Koziara,

Maria

et al. 2023

Foods

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Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One of the tools to narrow this gap could be microscopic imaging, as a convenient approach to visualize the abundance and distribution of dietNAs in food products. With the aid of appropriate bioinformatic elaboration, such images may in future enable the direct semiquantitative estimation of these macromolecules in food products. In the presented study, two methods of preparing microscopic sections and staining them with DNA-specific fluorochromes were used for microscopic imaging of dietNAs in food products of plant and animal origin. Procedures for preparing formalin-fixed paraffin-embedded sections and cryosections were compared in terms of their usefulness for routine food analysis. Both methods turned out equally suitable for visualizing dietNA distribution in animal and plant products. However, the use of cryosections allowed a significantly shorter analysis time and reduced the consumption of organic solvents. Both of these advantages make the cryosection method preferable while establishing a dedicated methodology for routine assessment of dietNAs in the food industry.

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Section: Introductionmentioning

confidence: 99%

Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins

Kościelak,

Koziara,

Maria

et al. 2023

Foods

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“…Conventional pre-clinical and toxicological practices are often based on decades old methodologies of animal trials and use of tumor-derived cell lines, delivering questionable and unreliable results [ 7 ]. In vitro models of normal human tissues are therefore strongly gaining importance due to their relevance and wide applicability that ranges from mechanistic studies to risk assessment [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Gut Cell Models for Toxicological and Bioactivity Studies of Functional and Novel Foods

Trapečar

Cencič

2012

Foods

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The concept of functional and novel foods undoubtedly bears great potential as an asset to human health. However, this very same quest for ever new bioactive ingredients calls for reliable and distinct risk assessment as they may be potentially hazardous to human health. Most of today’s methodologies still rely on decades old routines of animal trials and use of tumor-derived cell lines. Since such methodologies are not in line with the actual processes in the human body and with the 3R (replacement, reduction, refinement) concept, the results are often unreliable and misleading. Therefore, in this paper we propose the utilization of available untransformed small intestinal cell lines derived from human and pig tissue of non-tumor origin and describe several available cell models of the gut that offer a functional, close resemblance with the in vivo environment.

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State of the art in benefit–risk analysis: Food and nutrition

Tijhuis

Jong

Pohjola

et al. 2012

Food and Chemical Toxicology

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A critique of prevailing approaches to nutrient risk analysis pertaining to food supplements with specific reference to the European Union

Cited by 7 publications

References 48 publications

Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins

Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins

Application of Gut Cell Models for Toxicological and Bioactivity Studies of Functional and Novel Foods

State of the art in benefit–risk analysis: Food and nutrition

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