Background: To combat the reproductive and child health (RCH) problems, National Rural Health Mission (NRHM) had launched Janani Suraksha Yojana (JSY) in April 2005 in all states and union territories to promote institutional deliveries among the poor population through provision of antenatal, intra-natal and post-natal care services for women to have healthy outcomes of pregnancy and child birth. Hence this study has been undertaken on one of the important aspect of services that is antenatal care services with the objective to assess the utilization of antenatal care services among JSY beneficiaries in rural area.Methods: Cross sectional observational study done in the area under rural health training centre of a private medical college of Pune district. 1st October 2014 to 30th September 2015. Study sample: All JSY beneficiaries who delivered during study period (1st October 2014 to 30th September 2015). One to one interview with the all 155 beneficiaries was conducted using the pretested, questionnaire. The interview was timed at minimum 6 week interval after the delivery. Data analysis was done by using SPSS 20.0 statistical software.Results: Majority 78 (50.3%) of beneficiaries out of 155 got registered themselves within 12 weeks of pregnancy. Antenatal coverage was quite good. Coverage of injection tetanus toxoid was 100% and all essential investigation have been carried out. Association between literacy level (up to higher secondary level) of beneficiaries and number of ANC visits was found statistically significant (p<0.001).Conclusions: In present study utilization of ANC services was found to be good.