A Cu/Zn Superoxide Dismutase Gene From Saussurea involucrata Kar. et Kir., SiCSD, Enhances Drought, Cold and Oxidative Stress in Transgenic Tobacco

Zhang, Lili; Sun, Lingyan; Qiu, Honglin; Liu, C.; Wang, A.; Deng, Fenni; Zhu, Jianbo

doi:10.1139/cjps-2016-0180

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…The superoxide radical formed due to oxidative stress is converted into H 2 O 2 and molecular oxygen with the enzyme Cu/Zn-SOD and provides the conversion of H 2 O 2 to oxygen and water with APX and CAT enzymes. In plants, an increase in Cu/Zn-SOD gene expression has been reported under abiotic and biotic stress conditions (Van Camp et al, 1994;Wu, et al, 2016;Zhang et al, 2017). In this study, SLCu/Zn-SOD gene encoding Cu/Zn-SOD3 form was studied in L. esculentum.…”

Section: Rna Isolation and Qpcr Analysismentioning

confidence: 85%

Effect of proteasome inhibitor MG132 on the expression of oxidative metabolism related genes in tomato

Karpuz

Çakır

2022

Food Sci. Technol

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Expression analysis of antioxidant related genes may provide key information to develop plants more tolerant to abiotic and biotic stress. For this purpose, in this study, proteosome was inhibited by using MG132 proteosome inhibitor in Lycopersicon esculentum (tomato) tissues with high antioxidant content. We aimed to explain the response of gene isoforms associated with oxidative stress metabolism as a result of proteasome inhibition induced by MG132 in tomato. Gene expression levels of SLGR1, SLPhGPX, SLCAT1, SLFe-SOD, SLGPX, SLCu/Zn-SOD, SLcAPX and SLGST genes which are known to be associated with antioxidant mechanisms, were determined by real-time PCR in tomato. There was no significant change in SLPhGPX, SLCAT1, SL-Fe-SOD and SLcAPX genes in root, stem and leaf tissues. In addition, expression of SLGR1 and SLGST genes in root tissue was increased. In leaf tissue, expression of SLGPX, SLCu/Zn-SOD and SLGST genes increased significantly. There was no significant change in the expression of the genes studied in the shoot tissue. These results obtained from tomato root, stem and leaf tissues by creating proteasome inhibition will help to understand the relationship between antioxidant system and proteasome system in plants.

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Section: Rna Isolation and Qpcr Analysismentioning

confidence: 85%

Effect of proteasome inhibitor MG132 on the expression of oxidative metabolism related genes in tomato

Karpuz

Çakır

2022

Food Sci. Technol

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“…The transcripts of SOD genes were found to be altered under different abiotic stresses (Wang et al 2017;Zhang et al 2017;Zhou et al 2017). To explore whether the MtSOD genes are related to abiotic stress responses or not, we analyzed the microarray and high-throughput sequencing data from M. truncatula.…”

Section: Discussionmentioning

confidence: 99%

In silico identification and expression analysis of superoxide dismutase (SOD) gene family in Medicago truncatula

et al. 2018

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Superoxide dismutase (SOD) proteins are crucial antioxidant enzymes that play critical roles in plant growth, development, and response to various abiotic stresses. The gene family has been characterized in various plant species, but not in yet. Here, a total of 7 genes were first identified from the whole genome of, including 1 , 2, and 4 /, which are unevenly distributed in five out of the eight chromosomes. Phylogenetic analysis showed that SOD proteins from and other plant species could be classified into two main categories (Cu/ZnSODs and Fe-MnSODs), which could be further divided into eight subgroups, and members within the same subgroup tended to share the same subcellular localization. In addition, genes together with and within the same subgroup also displayed similar motif compositions and exon-intron structures. Most genes were ubiquitously expressed in various tissues, particularly in leaves, seeds and root nodules at different developmental stages. Moreover, microarray analysis and high-throughput sequencing showed that most genes were differentially expressed under salt, drought, and cold treatments, indicating their pivotal roles in stress response of . These findings provide useful information for the functional characterization of family genes for growth, development, and stress response of .

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“…Increases in activity of SOD are highly protective against drought stress in various plant species ( Faize et al, ; Zhang et al, , and further references therein). According to Faize et al () high expression of SOD genes in seeds improved germination of seeds under unfavorable conditions.…”

Section: Discussionmentioning

confidence: 99%

Zinc‐biofortified seeds improved seedling growth under zinc deficiency and drought stress in durum wheat

Candan

Çakmak

Öztürk

2018

J. Plant Nutr. Soil Sci.

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High zinc (Zn) concentration of seeds has beneficial effects both on seed vigor and human nutrition. This study investigated the effect of Zn biofortification on growth of young durum wheat (Triticum durum cv. Yelken) seedlings under varied Zn and water supply. The seeds differing in Zn concentrations were obtained by spraying ZnSO4 to durum wheat plants at different rates under field conditions. Three groups of seeds were obtained with the following Zn concentrations: 9, 20, and 50 mg Zn kg−1. The seeds differing in Zn were tested for germination rate, seedling height, shoot dry matter production, and shoot Zn concentration under limited and well irrigated conditions in a Zn‐deficient soil with and without Zn application. In an additional experiment carried out in solution culture, root and shoot growth and superoxide dismutase activity (SOD) of seedlings were studied under low and adequate Zn supply. Low seed Zn concentration resulted in significant decreases in seedling height both in Zn‐deficient and sufficient soil, but more clearly under water‐limited soil condition. Decrease in seed germination due to low seed Zn was also more evident under limited water supply. Increasing seed Zn concentration significantly restored impairments in seedling development. Drought‐induced decrease in seedling growth at a given seed Zn concentration was much higher when soil was Zn‐deficient. Increasing seed Zn concentration also significantly improved SOD activity in seedlings grown under low Zn supply, but not under adequate Zn supply. The results suggest that using Zn‐biofortified seeds assures better seed vigor and seedling growth, particularly when Zn and water are limited in the growth medium. The role of a higher antioxidative potential (i.e., higher SOD activity) is discussed as a possible major factor in better germination and development of seedlings resulting from Zn‐biofortified seeds.

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A Cu/Zn Superoxide Dismutase Gene From Saussurea involucrata Kar. et Kir., SiCSD, Enhances Drought, Cold and Oxidative Stress in Transgenic Tobacco

Cited by 15 publications

References 34 publications

Effect of proteasome inhibitor MG132 on the expression of oxidative metabolism related genes in tomato

Effect of proteasome inhibitor MG132 on the expression of oxidative metabolism related genes in tomato

In silico identification and expression analysis of superoxide dismutase (SOD) gene family in Medicago truncatula

Zinc‐biofortified seeds improved seedling growth under zinc deficiency and drought stress in durum wheat

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