A cytosine methyltransferase ortholog dmtA is involved in the sensitivity of Aspergillus flavus to environmental stresses

Zhi, Qing-Qing; Li, Jie-Ying; Liu, Qiuyun; He, Zhu‐Mei

doi:10.1016/j.funbio.2017.02.001

Cited by 14 publications

(15 citation statements)

References 72 publications

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“…Previous studies have reported that methyltransferases participated in various stress responses in A. flavus (Liang et al, 2017;Zhi et al, 2017). Therefore, the potential role of AflO (O-methyltransferase) with response to cell wall integrity, genotoxic and oxidative stresses was examined.…”

Section: Aflo Response To Cell Wall Integrity Stress In a Flavusmentioning

confidence: 99%

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Guang

Yue

Ren

et al. 2019

Environmental Microbiology

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Summary Aspergillus flavus is a pathogenic fungus that produces carcinogenic aflatoxins, posing a great threat to crops, animals and humans. Lysine acetylation is one of the most important reversible post‐translational modifications and plays a vital regulatory role in various cellular processes. However, current information on the extent and function of lysine acetylation and aflatoxin biosynthesis in A. flavus is limited. Here, a global acetylome analysis of A. flavus was performed by peptide pre‐fractionation, pan‐acetylation antibody enrichment and liquid chromatography–mass spectrometry. A total of 1313 high‐confidence acetylation sites in 727 acetylated proteins were identified in A. flavus. These acetylation proteins are widely involved in glycolysis/gluconeogenesis, pentose phosphate pathway, citric acid cycle and aflatoxin biosynthesis. AflO (O‐methyltransferase), a key enzyme in aflatoxin biosynthesis, was found to be acetylated at K241 and K384. Deletion of aflO not only impaired conidial and sclerotial developments, but also dramatically suppressed aflatoxin production and pathogenicity of A. flavus. Further site‐specific mutations showed that lysine acetylation of AflO could also result in defects in development, aflatoxin production and pathogenicity, suggesting that acetylation plays a vital role in the regulation of the enzymatic activity of AflO in A. flavus. Our findings provide evidence for the involvement of lysine acetylation in various biological processes in A. flavus and facilitating in the elucidation of metabolic networks.

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Section: Aflo Response To Cell Wall Integrity Stress In a Flavusmentioning

confidence: 99%

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Guang

Yue

Ren

et al. 2019

Environmental Microbiology

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“…Fungal secondary metabolism and morphological development have been shown to be associated at the genetic level [ 30 , 31 , 32 ]; for example, in A. nidulans and in Aspergillus spp. producing aflatoxins, it was demonstrated that a FadA-dependent signal transduction pathway regulate both conidiation and sterigmatocystin-aflatoxins biosynthesis [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of the alb1 Orthologue Gene in the Ochratoxigenic Fungus Aspergillus carbonarius (AC49 strain)

Gerin

González-Candelas

Ballester

et al. 2018

Toxins

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Aspergillus carbonarius, belonging to the group Nigri, is the main species responsible for contamination by ochratoxin A (OTA) in grapes and derivative products. OTA can accumulate in the mycelium and in black conidia of the fungus and released into the matrix. Here, we have deleted in A. carbonarius the alb1 orthologue gene of A. fumigatus, involved in melanin biosynthesis. Three A. carbonarius Δalb1 mutants were characterized for morphologic traits and OTA production on different media and temperatures. Δalb1 mutants showed a fawn color of conidia associated with a significant reduction of the conidiogenesis and a statistically significant increase (p ≤ 0.01) of total OTA production as compared to the wild type (WT) strain. The alb1 gene somehow affected OTA partitioning since in Δalb1 mutants OTA amount was lower in conidia and was more abundantly secreted into the medium as compared to the WT. On grape berries the Δalb1 mutants and the WT caused lesions with similar sizes but OTA amount in berry tissues was higher for the mutants. These results demonstrate that A. carbonarius conidia pigmentation is largely dependent on polyketide biosynthesis. The gene is not directly involved in virulence and its deletion affects morphological features and OTA production in the fungus.

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“…In such situations, we believe that DNA methylation does not act to regulate the expressions of the genes involved in a latoxin pathways and is not a target of 5-AC. Moreover, we ind that DmtA, a DNA methyltransferase homologue, is important in regulation of a latoxin biosynthesis and for A. lavus to adapt to stressful environments and for survival [93]. This increase the complexity in the regulation of a latoxin biosynthesis.…”

Section: Prospectsmentioning

confidence: 93%

Advances in research of the structural gene characteristics of the aflatoxin biosynthetic gene cluster

Li¹,

He²

2018

J Plant Sci Phytopathol

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Afl atoxins, produced by Aspergillus spp., are strongly toxic and carcinogenic fungal secondary metabolites. Afl atoxin biosynthesis is a complex process and involves at least 30 genes clustered within an approximately 75 kB gene cluster. In this paper, we reviewed current status of the researches on the characterized structural genes involved in afl atoxin biosynthesis and their roles in afl atoxin-producing fungi, especially in A. fl avus and A. parasiticus, which will improve our understanding of the mechanism of afl atoxin biosynthesis and regulation and provide reference for further study.

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A cytosine methyltransferase ortholog dmtA is involved in the sensitivity of Aspergillus flavus to environmental stresses

Cited by 14 publications

References 72 publications

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Functional Characterization of the alb1 Orthologue Gene in the Ochratoxigenic Fungus Aspergillus carbonarius (AC49 strain)

Advances in research of the structural gene characteristics of the aflatoxin biosynthetic gene cluster

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