2019
DOI: 10.1186/s12870-018-1597-6
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A cytosolic NAD+-dependent GPDH from maize (ZmGPDH1) is involved in conferring salt and osmotic stress tolerance

Abstract: BackgroundPlant glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the reduction of dihydroxyacetone phosphate (DHAP) to produce glycerol-3-phosphate (G-3-P), and plays a key role in glycerolipid metabolism as well as stress responses.ResultsIn this study, we report the cloning, enzymatic and physiological characterization of a cytosolic NAD+-dependent GPDH from maize. The prokaryotic expression of ZmGPDH1 in E.coli showed that the enzyme encoded by ZmGPDH1 was capable of catalyzing the reduction of DHAP in t… Show more

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Cited by 25 publications
(23 citation statements)
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“…However, strain HX57D produced slightly more glycerol than HX62W after 24 h of fermentation (data not shown). It was speculated that the strain HX62W might retain most glycerol intracellularly for its role in osmo-tolerance [ 38 ]. The hypothesis was supported by the facts that the osmotic stress-related transcription factors (TFs), Cin5p and Mot3p, had higher expression levels in HX62W than in HX57D (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…However, strain HX57D produced slightly more glycerol than HX62W after 24 h of fermentation (data not shown). It was speculated that the strain HX62W might retain most glycerol intracellularly for its role in osmo-tolerance [ 38 ]. The hypothesis was supported by the facts that the osmotic stress-related transcription factors (TFs), Cin5p and Mot3p, had higher expression levels in HX62W than in HX57D (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In addition, Vigeolas et al [61] found that a twofold overexpression of the yeast gene coding for GPD1 led to a three- to fourfold increase in the level of G3P in transgenic Brassica napus seeds, resulting in a 40% increase in oil content, with the protein content remaining substantially unchanged. Overexpression of maize GPD1 remarkably enhanced the G3P production and the salinity/osmotic stress tolerance in transgenic Arabidopsis plants, and the transgenic lines showed no aberrant phenotype under normal growth conditions, no matter in the vegetative growth phase or reproductive developmental stages [62]. Overexpression of the yeast GPD1 increased Camelina sativa seed oil content about 5.4% but cause no change in seed protein content and no effect on seed germination and seedling growth [63].…”
Section: Discussionmentioning
confidence: 99%
“…Hou et al [62] evidenced no significant differences in GSH contents between shoots and roots of maize seedlings under mercury stress, but under vanadium-mercury combined stress the GSH content decreased with increasing Hg concentration. Upon salinity or osmotic treatment, the transcripts of genes related to the GSH biosynthesis were significantly increased in transgenic wild type of Arabidopsis thaliana L. [63]. During heat stress, GSH content in maize seedlings decreased, but NAHS-treated plants maintained higher GSH level than non-treated controls.…”
Section: Discussionmentioning
confidence: 99%