A database on the stability of silver and gold nanostructures for applications in biology and biomolecular sciences

Abstract

Colloidal stability of nanoparticles in biological media is an important consideration when trying to ensure reliable data interpretation of in vitro and in vivo systems. We have developed a detailed colloidal stability library of newly synthesized gold, silver and gold-core silver-shell plasmonic nanoparticles, stabilized with aspartame, glucosamine and sucralose, in various biologically relevant buffers and bacterial and mammalian cell culture media. The stabilizer selection reflects the preference for molecules that are non-toxic, inexpensive, readily available, water soluble and easy-to-replace if that is the end-user preference. An on-line resource provides detailed stability information on each of the 81 systems examined. To illustrate how to utilize this stability library, we conducted bacterial toxicity and biocompatibility experiments through the use of one specific set of nanomaterials in the presence and absence of plasmonic irradiation.

Biocompatibility and photo-induced antibacterial activity of lignin-stabilized noble metal nanoparticles

“…peptidoglycan layer present in the bacterial cell wall can help to attract sugars, and with them the nanocomposites attached. 17,47 As mentioned before, there are two main processes that are believed to be involved in the photo-induced antimicrobial activity of MNP, 12,13 namely, photothermal and photochemical mechanisms. The rst one involves the absorption of light by the MNP and the concomitant local release of heat from the particle that can destroy biomolecules (or melt the lipids in a cell membrane) in close proximity to the MNP surface.…”

“…Coincidentally, these nanocomposites contain lignin with higher sugar content, which could contribute to a better interaction with the S. aureus bacterial cell wall. 17,47 Additionally, Fig. S9 † shows the AuNP@lignin tested are non-toxic for the bacteria in the dark, unless they are subjected to light exposure.…”

“…12,13 The growing interest in the use of environmentally friendly capping agents, as well as, the use of sustainable resources for antimicrobial applications, 14,15 such as food packaging, 16 has led our group towards the use of non-toxic and inexpensive capping agents. 17 Here we explore the use of lignin, the second most abundant natural polymer on earth aer cellulose, as an alternative reducing and capping agent for the synthesis and stabilization of metal nanoparticles for potential applications as antimicrobial agents.…”

Anti-biofilm effects of gold and silver nanoparticles synthesized by the Rhodiola rosea rhizome extracts

“…Essam et al reported that AgNPs produced by Bacillus licheniformis showed that the zeta potential ranged from À16.6 to À21.3 mV was quite stable, demonstrated excellent in vitro antimicrobial activity against important human pathogens and antiviral activity against the Bean Yellow Mosaic Virus [41]. Thus, the negative surface charge is highly responsible for providing the colloidal stability to nanoparticles, especially in biological environments [42].…”

Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

“…Stability was considered as the time it took for a 20% decrease in plasmon absorption at the wavelength of the initial maximum absorbance. 8 The stability measurements were carried out (using a BioTek plate reader) for 7 days (time = 0, 0.5, 1, 2, 4, 6, 12 18, 24, 48, 72, 120, 144, 168 h) in Milli-Q water, saline solution, PBS (pH = 7), Mueller Hinton broth (MHB), and tryptic soy broth (TSB) at different concentrations (100, 50, 25, 12.5%). To avoid contamination, the stability measurement of nanoparticles dispersed in growth media was conducted under sterile conditions.…”