A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat

Wang, Yi-Xue; Xu, Qiao-fang; Chang, Xiaoping; Hao, Chenyang; Li, Runzhi; Jing, Ruilian

doi:10.1016/s2095-3119(17)61685-x

Cited by 12 publications

(11 citation statements)

References 34 publications

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“…From 1970s to 1990s, the frequency of favorable allelic variation TaPPH-7A-1 (A) maintained a relatively stable level about 56%, but TGW continuously increased (Fig. 7), the possible reason may be that other genes contributing to TGW were selected, such as TaSnRK2.3-1A ( Hap-1A-1 ) and TaSnRK2.3-1B ( Hap-1B-1 ) [56], TaSPL21-6D-Hap II&III [57], and TaSAP7-B (C) [58]. Moreover, the frequency of favorable allelic variation TaPPH-7A-1 (A) was increased from landraces (Population 2) to modern cultivars (Population 3) in Chinese ten wheat Zones.…”

Section: Discussionmentioning

confidence: 99%

Identification of TaPPH-7A haplotypes and development of a molecular marker associated with important agronomic traits in common wheat

Wang

Chang

et al. 2019

BMC Plant Biol

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Background Premature senescence of flag leaf severely affects wheat yield and quality. Chlorophyll (Chl) degradation is the most obvious symptom during leaf senescence and catalyzed by a series of enzymes. Pheophytin pheophorbide hydrolase (Pheophytinase, PPH) gene encodes a Chl degradation hydrolase. Results In this study, the coding, genomic and promoter sequences of wheat TaPPH-A gene were cloned. The corresponding lengths were 1467 bp, 4479 bp and 3666 bp, respectively. Sequence structure analysis showed that TaPPH-A contained five exons and four introns. After the multiple sequences alignment of TaPPH-A genome from 36 accessions in a wheat diversity panel, four SNPs and one 2-bp InDel were observed, which formed two haplotypes, TaPPH-7A-1 and TaPPH-7A-2 . Based on the SNP at 1299 bp (A/G), a molecular marker TaPPH-7A -dCAPS was developed to distinguish allelic variation (A/G). Using the molecular markers, 13 SSR, and 116 SNP markers, a linkage map of chromosome 7A were integrated. TaPPH-A was mapped on the chromosome region flanked by Xwmc9 (0.94 cM) and AX-95634545 (1.04 cM) on 7A in a DH population. Association analysis between TaPPH-7A allelic variation and agronomic traits found that TaPPH-7A was associated with TGW in 11 of 12 environments and Chl content at grain-filling stage under drought stress using Population 1 consisted of 323 accessions. The accessions possessed TaPPH-7A-1 (A) had higher TGW and Chl content than those possessed TaPPH-7A-2 (G), thus TaPPH-7A-1 (A) was a favorable allelic variation. By analyzing the frequency of favorable allelic variation TaPPH-7A-1 (A) in Population 2 with 157 landraces and Population 3 with 348 modern cultivars, we found it increased from pre-1950 (0) to 1960s (54.5%), then maintained a relatively stable level about 56% from 1960s to 1990s. Conclusion These results suggested the favorable allelic variation TaPPH-7A-1 (A) should be valuable in enhancing grain yield by improving the source (chlorophyll content) and sink (the developing grain) simultaneously. Furthermore, the newly developed molecular marker TaPPH-7A -dCAPS could be integrated into a breeding kit of screening high TGW wheat for marker-assisted selection. Electronic supplementary material The online version of this article (10.1186/s12870-019-1901-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Identification of TaPPH-7A haplotypes and development of a molecular marker associated with important agronomic traits in common wheat

Wang

Chang

et al. 2019

BMC Plant Biol

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“…The given Pakistani wheat germplasm had high frequencies of unfavorable allelic variations for TaMOC-7A and TaPARG-2A, suggesting the potential of favorable haplotype introgression through functional markers. Previously, selection pressure was observed on TaSnRK2.3-1A/B (Miao et al, 2017), TaSnRK2.9-5A (Ur Rehman et al, 2019), TaSAP-7B (Wang et al, 2018), TaMOC-7A , TaLTPs-1A , TaPARG-2A (Li B. et al, 2016), and TaPPH-7A (Wang et al, 2019) favorable allelic variations in Chinese wheat cultivars where the frequencies of favored haplotypes had gradually increased from the beginning of the last century. Both PIC and H e values were higher in tetraploid wheat as compared to Pakistani hexaploid wheat.…”

Section: Allelic Variation At Loci Influencing Grain Related Traitsmentioning

confidence: 97%

“…TaSnRK2.3-1A/1B (Miao et al, 2017), TaSnRK2.9-5A (Ur Rehman et al, 2019), TaPARG-2A (Li B. et al, 2016), TaSAP-7B (Wang et al, 2018), TaPPH-7A (Wang et al, 2019), and TaMOC1-7A are associated with higher grain yield under water stress conditions. TaSnRK2.8-5A associated with higher seedling biomass under normal conditions and water-soluble carbohydrates under limited irrigation conditions (Zhang et al, 2013), TaLTPs-1A associated with ideal plant height under drought conditions , and TaDreb-B1 (Wei et al, 2009) and 1-feh w3 are also reported as drought toleranceconferring genes.…”

Section: Introductionmentioning

confidence: 99%

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Development and Exploitation of KASP Assays for Genes Underpinning Drought Tolerance Among Wheat Cultivars From Pakistan

et al. 2021

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High-throughput genotyping for functional markers offers an excellent opportunity to effectively practice marker-assisted selection (MAS) while breeding cultivars. We developed kompetitive allele-specific PCR (KASP) assays for genes conferring drought tolerance in common wheat (Triticum aestivum L.). In total, 11 KASP assays developed in this study and five already reported assays were used for their application in wheat breeding. We investigated alleles at 16 loci associated with drought tolerance among 153 Pakistani hexaploid wheat cultivars released during 1953–2016; 28 diploid wheat accessions (16 for AA and 12 for BB) and 19 tetraploid wheat (AABB) were used to study the evolutionary history of the studied genes. Superior allelic variations of the studied genes were significantly associated with higher grain yield. Favored haplotypes of TaSnRK2.3-1A, TaSnRK2.3-1B, TaSnRK2.9-5A, TaSAP-7B, and TaLTPs-1A predominated in Pakistani wheat germplasm indicating unconscious pyramiding and selection pressure on favorable haplotypes during selection breeding. TaSnRK2.8-5A, TaDreb-B1, 1-feh w3, TaPPH-7A, TaMOC-7A, and TaPARG-2A had moderate to low frequencies of favorable haplotype among Pakistani wheat germplasm pointing toward introgression of favorable haplotypes by deploying functional markers in marker-assisted breeding. The KASP assays were compared with gel-based markers for reliability and phenotypically validated among 62 Pakistani wheat cultivars. Association analyses showed that the favorable allelic variations were significantly associated with grain yield-contributing traits. The developed molecular marker toolkit of the genes can be instrumental for the wheat breeding in Pakistan.

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“…The functional marker of TaSAP7-B was identified by the dCAPS marker, which was significantly associated with 1,000-grain weight and plant height. The superior allelic variation of TaSAP7-B was apparently selected in wheat-breeding programs ( Wang et al, 2018 ). The present developed functional markers of TaSAP7-A , which associated with 1,000-grain weight, plant height, and chlorophyll content at the jointing stage and grain-filling stage, could be used in target trait selection in wheat-breeding programs.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of TaSAP7-A, a Member of the Stress-Associated Protein Family in Common Wheat

Wang

et al. 2021

Front. Plant Sci.

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Stress association proteins (SAPs) are A20/AN1 zinc-finger domain proteins, which play important roles in plant adaptation to abiotic stress and plant development. The functions of SAPs in some plants were reported, but little is known about it in wheat (Triticum aestivum L.). In this study, we characterized a novel 2AN1-type stress association protein gene TaSAP7-A, which was mapped to chromosome 5A in wheat. Subcellular localization indicated that TaSAP7-A was distributed in the nucleus and cytoplasm. Unlike previously known A20/AN1-type SAP genes, TaSAP7-A was negatively regulated to abiotic stress tolerance. Overexpressing TaSAP7-A Arabidopsis lines were hypersensitive to ABA, osmotic and salt stress at germination stage and post-germination stage. Overexpression of TaSAP7-A Arabidopsis plants accelerated the detached leaves’ chlorophyll degradation. Association analysis of TaSAP7-A haplotypes and agronomic traits showed that Hap-5A-2 was significantly associated with higher chlorophyll content at jointing stage and grain-filling stage. These results jointly revealed that TaSAP7-A is related to the chlorophyll content in the leaves of Arabidopsis and wheat. Both in vivo and in vitro experiments demonstrated that TaSAP7-A interacted with TaS10B, which was the component of regulatory subunit in 26S proteasome. In general, TaSAP7-A was a regulator of chlorophyll content, and favorable haplotypes should be helpful for improving plant chlorophyll content and grain yield of wheat.

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A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat

Abstract: ScienceDirect A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat WANG Yi-xue 1 , XU Qiao-fang 2 , CHANG Xiao-ping 2 , HAO Chen-yang 2 , LI Run-zhi 1 , JING Rui-lian 2

Cited by 12 publications

References 34 publications

Identification of TaPPH-7A haplotypes and development of a molecular marker associated with important agronomic traits in common wheat

Identification of TaPPH-7A haplotypes and development of a molecular marker associated with important agronomic traits in common wheat

Development and Exploitation of KASP Assays for Genes Underpinning Drought Tolerance Among Wheat Cultivars From Pakistan

Cloning and Characterization of TaSAP7-A, a Member of the Stress-Associated Protein Family in Common Wheat

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