A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)

Gioacchini, Giorgia; Marisaldi, Luca; Basili, Danilo; Candelma, Michela; Pignalosa, Paolo; Cigliano, Riccardo Aiese; Sanseverino, Walter; Hardiman, Gary; Carnevali, Oliana

doi:10.1038/s41598-019-43872-6

Cited by 13 publications

(14 citation statements)

References 81 publications

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“…Our DEAS analysis showed that the Cassette event was the most frequent DEAS event in the hypothalamus and the pituitary, but not in the ovary, indicating that AS events might play different regulatory roles within the pubertal HPO axis. We found that the staged DEAS events were likely to occur on genes involving the signaling pathways that have all been reported to regulate the timing of puberty (Parent et al, 2008;Sun et al, 2009;Singh et al, 2015;Aliberti et al, 2019;Gioacchini et al, 2019). For example, previous studies have shown that tight junctions form in cells to create a major component of the blood-testis barrier during puberty in males (McCabe et al, 2012), and the blood-brain barrier also plays a crucial role in the microenvironment required to maintain neuronal function at puberty (Seker et al, 2016).…”

Section: Discussionmentioning

confidence: 79%

Alternative Splicing Dynamics of the Hypothalamus–Pituitary–Ovary Axis During Pubertal Transition in Gilts

Pan

Chen

et al. 2021

Front. Genet.

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The timing of puberty in mammals marks the point at which reproduction becomes possible. Abnormalities in the timing of puberty may exert a series of negative effects on subsequent health outcomes. Alternative splicing (AS) has not only emerged as a significant factor in the transcription of genes but it is also reported to play a role in the timing of puberty. However, to date, the changes and dynamics of AS during the onset of puberty is extremely seldom explored. In the present study, we used gilts as a research model to investigated the dynamics of AS and differentially expressed AS (DEAS) events within the hypothalamus–pituitary–ovary (HPO) axis across pre-, in-, and post-puberty. We detected 3,390, 6,098, and 9,085 DEAS events in the hypothalamus, pituitary, and ovary when compared across pre-, in-, and post-pubertal stages, respectively. Within the entire HPO axis, we also identified 22,889, 22,857, and 21,055 DEAS events in the pre-, in-, and post-pubertal stages, respectively. Further analysis revealed that the differentially spliced genes (DSGs) associated with staged DEAS events were likely to be enriched in the oxytocin signaling pathway, thyroid hormone signaling pathway, GnRH signaling pathway, and oocyte meiosis signaling pathway. The DSGs associated with DEAS events across the entire HPO axis were enriched in endocytosis signaling pathway, the MAPK signaling pathway, and the Rap1 signaling pathway. Moreover. the ASs of TAC1, TACR3, CYP19A1, ESR1, ESRRA, and FSHR were likely to regulate the functions of the certain HPO tissues during the onset of puberty. Collectively, the AS dynamics and DEAS events were comprehensively profiled in hypothalamus, pituitary, and ovary across the pre-, in-, and post-pubertal stages in pigs. These findings may enhance our knowledge of how puberty is regulated by AS and shed new light on the molecular mechanisms underlying the timing of puberty in mammals.

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Section: Discussionmentioning

confidence: 79%

Alternative Splicing Dynamics of the Hypothalamus–Pituitary–Ovary Axis During Pubertal Transition in Gilts

Pan

Chen

et al. 2021

Front. Genet.

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“…Similarly, as previously observed in fish treated with L. rhanmosus [14,16], also SLAb51 administration stimulated certain forms of vtg. Focusing on vitellogenesis, in oviparous vertebrates this process starts in the liver and is triggered by gonadal estrogens [41,42]. Zebrafish contain 8 different forms of vitellogenin genes [43] encoding for 3 proteins including type I (vtg1, vtg4, vtg5, vtg6, and vtg7), type II (vtg2 and vtg8) and type III (vtg3) [44], which differently contribute to the embryonic morphogenesis, hatching, larval kinetics and survival (vtg1, 3, 4, and 5) or provide homeostatic regulation of total vtg levels (vtg7) [45,46].…”

Section: Discussionmentioning

confidence: 99%

Probiotic Administration Mitigates Bisphenol A Reproductive Toxicity in Zebrafish

Giommi

Habibi

Candelma

et al. 2021

IJMS

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Although the use of bisphenol A (BPA) has been banned in a number of countries, its presence in the environment still creates health issues both for humans and wildlife. So far, BPA toxicity has been largely investigated on different biological processes, from reproduction to development, immune system, and metabolism. In zebrafish, Danio rerio, previous studies revealed the ability of environmentally relevant concentrations of this contaminant to significantly impair fertility via epigenetic modification. In addition, several studies demonstrated the ability of different probiotic strains to improve organism health. This study provides information on the role of the probiotic mixture SLAb51 to counteract adverse BPA effects on reproduction. A 28-day trial was set up with different experimental groups: BPA, exposed to 10 µg/L BPA; P, receiving a dietary supplementation of SLAb51 at a final concentration of 109 CFU/g; BPA+P exposed to 10 µg/L BPA and receiving SLAb51 at a final concentration of 109 CFU/g and a C group. Since oocyte growth and maturation represent key aspects for fertility in females, studies were performed on isolated class III (vitellogenic) and IV (in maturation) follicles and liver, with emphasis on the modulation of the different vitellogenin isoforms. In males, key signals regulating spermatogenesis were investigated. Results demonstrated that in fish exposed to the combination of BPA and probiotic, most of the transcripts were closer to C or P levels, supporting the hypothesis of SLAb51 to antagonize BPA toxicity. This study represents the first evidence related to the use of SLAb51 to improve reproduction and open new fields of investigation regarding its use to reduce endocrine disrupting compound impacts on health.

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“…Small portions of ovary (2 cm 3 ) were excised and fixed in a formaldehyde/glutaraldehyde solution (NaH 2 PO 4– H 2 O + NaOH + formaldehyde (36.5%) + Gluteraldehyde (25%) + H 2 O) and kept at +4°C until histological analysis. Briefly, following Gioacchini et al (), samples were dehydrated in a series of increasing EtOH baths, cleared in xylene, and embedded in paraffin. Sections of 5 μm in width were cut with a microtome (model RM2125 RTS; Leica Biosystems, Wetzlar, Germany), stained with Mayer's haematoxylin/eosin, and examined under a microscope (Axio Imager 2; Zeiss, Oberkochen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Maturity assignment based on histology‐validated macroscopic criteria: Tackling the stock decline of the Mediterranean swordfish (Xiphias gladius)

Marisaldi

Basili

Candelma

et al. 2019

Aquatic Conservation

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Effective stock assessment relies on the evaluation of the reproductive potential of a population, but this is often achieved using fast, inexpensive, and inaccurate macroscopic indicators of gonadal maturity. Indeed, this approach suffers from high error rates as a result of misclassification and operator errors. Although histology is considered the gold standard to assign reproductive stages, it represents a time‐consuming and expensive method. Therefore, cost‐effective approaches are currently needed to assess the species‐specific reproductive potential and define the correct size at first maturity (L50). In the present study a histology‐calibrated method based on the gonadal index (GI) was developed to assign maturity stages to the overfished Mediterranean swordfish (Xiphias gladius), supported by histological classification of gonadal development, an estimation of L50, and an analysis of the reproductive output of this species. The performance of this new method had an overall agreement of 90% with histological data, and the L50 calculated by the GI‐based method compared with histology analysis was not statistically different (p > 0.05). Moreover, in order to apply this method without bias, a thorough validation of the relationship between length and gonad weight was carried out and the suitability of the GI to compare individuals of different size and maturity stages was confirmed. The present findings provide a comprehensive picture of the patterns characterizing Mediterranean swordfish reproduction and a promising method to assign maturity stages based on a macroscopic indicator. This method has the potential to be extensively applied during routine fish sampling activities in the field because of its simple requirements.

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A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)

Cited by 13 publications

References 81 publications

Alternative Splicing Dynamics of the Hypothalamus–Pituitary–Ovary Axis During Pubertal Transition in Gilts

Alternative Splicing Dynamics of the Hypothalamus–Pituitary–Ovary Axis During Pubertal Transition in Gilts

Probiotic Administration Mitigates Bisphenol A Reproductive Toxicity in Zebrafish

Maturity assignment based on histology‐validated macroscopic criteria: Tackling the stock decline of the Mediterranean swordfish (Xiphias gladius)

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