A Decision Tree based Real-time Hand Gesture Recognition Method using Kinect

Chang, Guochao; Park, Jaewan; Oh, Chi-Min; Lee, Chil-Woo

doi:10.9717/kmms.2013.16.12.1393

Cited by 9 publications

(10 citation statements)

References 15 publications

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“…In order to broaden the applicability of ICPMS-based methods in quantitative protein studies, concepts were developed for their sensitive detection after chemical labelling with an ICPMS detectable element [19,20], Several methods were suggested, either using the labelling of antibodies [21] or the direct labelling of proteins or peptides [22]. In contrast to metals naturally incorporated in a protein, these labels can exhibit a higher stability under typically applied separation conditions, as for example, denaturing SDS-PAGE.…”

Section: Introductionmentioning

confidence: 99%

Systematic studies on the determination of Hg-labelled proteins using laser ablation-ICPMS and isotope dilution analysis

et al. 2011

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A method was developed for the precise and accurate determination of ovalbumin labelled with p-hydroxy-mercuribenzoic acid (pHMB) using polyacrylamide gel electrophoresis with ns-laser ablation-inductively coupled plasma mass spectrometry. Following systematic optimisation of the ablation process in terms of detection sensitivity, two different quantification strategies were applied: external calibration using standards of the derivatized protein after (13)C(+) normalization and, as a proof of concept, label-specific isotope dilution analysis (IDA) using pHMB enriched in the isotope (199)Hg. Due to the inhomogeneous distribution of the protein within the gel bands, it could be demonstrated that the IDA approach was superior in terms of precision and accuracy. Furthermore, it permits a reliable quantification, if more complex separation protocols are applied, as typically occurring analyte loss and degradation can be compensated for as soon as complete mixture of spike and sample is achieved. The estimated limit of detection was 160 fmol in the case of ovalbumin. In contrast to earlier studies using metals naturally present in proteins, no loss of mercury was observed during separation under denaturing conditions and other sample preparation steps. Using label-specific IDA, the measured isotope ratios in the gel corresponded to recoveries between 95% and 103%.

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Section: Introductionmentioning

confidence: 99%

Systematic studies on the determination of Hg-labelled proteins using laser ablation-ICPMS and isotope dilution analysis

et al. 2011

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“…There are numerous devices for the tasks: wearable devices like a ring [5], armband [6], glove [7], leap motion [8], and controllers based motion recognition such as Wii-mote [9], and ordinary web-camera [3], stereo camera [10], and even using radar [11]. Every technique has pros and cons.…”

Section: Related Workmentioning

confidence: 99%

“…There are many solutions to enhance the effectiveness of slide presentations [1,2,3]. Gesturebased slide control is among the most natural ways of Human Computer Interaction (HCI) [4].…”

Section: Introductionmentioning

confidence: 99%

Finger-Pointing Gesture Analysis for Slide Presentation

Harika¹,

Prihatmanto²,

Hindersah³

et al. 2016

Journal of Korea Multimedia Society

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This paper presents a method for computer-assisted slide presentation using vision-based gesture recognition. The proposed method consists of a sequence of steps, first detecting a hand in the scene of projector beam, then estimating the smooth trajectory of a hand or a pointing finger using Kalman Filter, and finally interfacing to an application system. Additional slide navigation control includes moving back and forth the pages of the presentation. The proposed method is to help speakers for an effective presentation with natural improved interaction with the computer. In particular, the proposed method of using finger pointing is believed to be more effective than using a laser pointer since the hand, the pointing or finger are more visible and thus can better grab the attention of the audience.

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“…20 Using the inorganic standards as the calibration, the absolute quantification of the target protein could be achieved by ICP-MS. Since the first paper on an ICP-MS based immunoassay with metal-tagging was published in 2001, 21 quantification of proteins by ICP-MS with element tags has emerged rapidly. Element-tagging plays a great role in taking full advantage of element-tagged protein quantification with ICP-MS detection.…”

Section: Introductionmentioning

confidence: 99%

Application of inductively coupled plasma mass spectrometry in the study of apoptosis: determination of caspase-3 using a gold nanoparticle tag

Liu

Chen

et al. 2016

Analyst

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Apoptosis is a main type of cell death in which caspase-3 plays a key role. In this work, a simple, fast and sensitive immunoassay for caspase-3 is established by using inductively coupled plasma mass spectrometry (ICP-MS) detection and signal enhancement gold nanoparticle (Au-NP) labelling of the secondary antibody (IgG). After the immunoreaction of caspase-3, primary antibody and Au-NP labeled IgG, the concentration of caspase-3 was determined by ICP-MS analysis of the Au-NPs released from the immunocomplex. Under the optimized conditions, a limit of detection (LOD, 3σ) of 0.42 ng mL(-1) (0.31 nM) was obtained for caspase-3, with a dynamic range of 1-200 ng mL(-1) and a relative standard deviation of 4.1% (c = 5 ng mL(-1), n = 11). The proposed method has good specificity towards caspase-3. To demonstrate the application potential of the proposed method, the cell lysates of Hg(2+)-induced HepG2 cells were analyzed, and it was found that the concentration of caspase-3 increased when increasing the incubation concentration of Hg(2+). This method is easy, sensitive and selective, and has excellent capability of tolerating a complex biological matrix, indicating the potential of the ICP-MS-based assay in the study of apoptosis.

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A Decision Tree based Real-time Hand Gesture Recognition Method using Kinect

Cited by 9 publications

References 15 publications

Systematic studies on the determination of Hg-labelled proteins using laser ablation-ICPMS and isotope dilution analysis

Systematic studies on the determination of Hg-labelled proteins using laser ablation-ICPMS and isotope dilution analysis

Finger-Pointing Gesture Analysis for Slide Presentation

Application of inductively coupled plasma mass spectrometry in the study of apoptosis: determination of caspase-3 using a gold nanoparticle tag

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