The goal of this study was to determine the anthropization evolution of the Guamá river basin in the years 2000, 2008 and 2018 by means of the Anthropic Transformation Index. Land use and cover maps were obtained from two databases, Project Mapbiomas (Brazilian Annual Land Use and Land Cover Mapping Project) and PRODES (Project for the Satellite Monitoring of the Brazilian Amazon Forest). The main classes defined in the mapping process are: forest, natural non-forest vegetation, agriculture and livestock farming, secondary vegetation, urban infrastructure, water and others. Secondary vegetation was considered as the area where the forest classes of Mapbiomas intersects with the deforested areas of PRODES, as determined by the map algebra operator. The expansion of agriculture and livestock farming achieved an increase of about 10%, while the forest was reduced in almost 10%. The Guamá river basin obtained an Anthropic Transformation Index of 4.44 in 2000, 5.04 in 2008 and 5.09 in 2018, going from a regular to a degraded state in 18 years. The occupation process caused major alterations in the natural components of the landscape over the course of 18 years, notably in the amount of forest. Protection of 35% of the remnant primary forest in the Guamá river basin is vital for the conservation of water resources vulnerable to changes in land use.