A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine

Abstract

A dendrimer-based sandwich type enzyme-linked immunosorbent assay (ELISA) was developed for the improved detection of recombinant human tumor necrosis factor-alpha (TNF-α) for early diagnosis of perinatal diseases. Hydroxyl-terminated generation four poly(amidoamine) dendrimer (G4-OH) was used for the development of a solid phase bio-sensing platform. The surface of the ELISA plate was modified with polyethylene-glycol (PEG) and thiol-functionalized G4-OH was immobilized on the PEG-functionalized plate. A capture antibody was oxidized and covalently immobilized onto the dendrimer-modified ELISA plate, which provides favorable orientation for the antigen binding sites towards the analyte. The dendrimer-modified plate showed enhanced sensitivity, and the detection limit for TNF-α was found to be 0.48 pg mL−1, which is significantly better than the commercially available ELISA kit. The selectivity of the dendrimer-modified ELISA plate was further evaluated with a mixture of cytokines, which showed results for similar to that of TNF-α alone. The modified plate provides a greater opportunity for the detection of a wide range of cytokines and biomarkers.

Is an episode of suspected preterm labor that subsequently leads to a term delivery benign?

“…The totality of the evidence suggests the following: 1) patients with an episode of suspected preterm labor may have subclinical intra-amniotic inflammation of unknown etiology, which has recently been attributed to danger signals (67–69); 2) some of these patients do not progress to preterm delivery and may enter a state of chronic intra-amniotic inflammation to which the fetus may be exposed; 3) experimental evidence in animal models shows that this process can lead to fetal neuroinflammation and damage (65); and 4) interestingly, this process can be subject to treatment with targeted nanodevices (i.e., dendrimers) if detected early (70–75). …”

Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva

“…39 The LOD of ELISA for TNF-α was reported to be 0.48 pg/mL in the buffer. 40 Compared with other biosensors for the detection of IL-8 or TNF-α, our method has not only better sensitivity, but also multiplexed detection ability. The sensitivity of the SiNW FET biosensor for IL-8 and TNF-α could also satisfy the requirement of diagnosing the OSCC at a premature stage.…”

Preparation of novel dendritic flame‐retardant polymer and its application in EPDM composites

“…However, the molecular weight, 525.2, 671.3, and 671.3, was obviously lower compared with the calculated molecular weight, 827, 2099, and 4643 Da. The reason for such a decrease may be ascribed to the generational defects and branching defects came from following aspects . First, some THPC molecules may turn into derivatives, for example, tetrakis (hydroxymethyl) phosphonium, the oxidation of trimethylolphosphine (THPO), the product of tris(hydroxymethyl) phosphine .…”