A description of the hepatitis B virus genomic background in a high-prevalence area in China

Chen, Xiaoming; Gao, Jie; Ji, Zhenyu; Zhang, Weilu; Zhang, Lei; Xu, Rui; Zhang, Jingxia; Li, Fēi; Shi, Li; Hu, Shijie; Shang, Lei; Shao, Zhongjun; Yan, Yongping

doi:10.1186/1743-422x-11-101

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…The three most prevalent serotypes were adr (36%), adw2 (29%) and ayw1 (19.9%). We found serotypes adr, ayr and ayw3 most commonly associated with genotype C, adw2 for B and C, adw3 and ayw1 for B, and ayw2 for D. These findings differ to Norder's study [13], which reported that the serotypes adw2, ayw1 and ayw3 were more pronounced amongst genotypes A, B and D. Moreover, our observations of HBsAg subtype distribution for genotypes B and D are dissimilar to the finding of Chen et al [71] who reported that adr and ayw2 were prevalent among genotype B strains while most of genotype D sequences were assigned to adr [71]. This is likely due to the geographical selection of HBV sequences used in this study.…”

Section: Discussioncontrasting

confidence: 99%

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In Silico Analysis of Genetic Diversity of Human Hepatitis B Virus in Southeast Asia, Australia and New Zealand

Phan

Faddy

Flower

et al. 2020

Viruses

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The extent of whole genome diversity amongst hepatitis B virus (HBV) genotypes is not well described. This study aimed to update the current distribution of HBV types and to investigate mutation rates and nucleotide diversity between genotypes in Southeast Asia, Australia and New Zealand. We retrieved 930 human HBV complete genomes from these regions from the NCBI nucleotide database for genotyping, detection of potential recombination, serotype prediction, mutation identification and comparative genome analyses. Overall, HBV genotypes B (44.1%) and C (46.2%) together with predicted serotypes adr (36%), adw2 (29%) and ayw1 (19.9%) were the most commonly circulating HBV types in the studied region. The three HBV variants identified most frequently were p.V5L, c.1896G>A and double mutation c.1762A>T/c.1764G>A, while genotypes B and C had the widest range of mutation types. The study also highlighted the distinct nucleotide diversity of HBV genotypes for whole genome and along the genome length. Therefore, this study provided a robust update to HBV currently circulating in Southeast Asia, Australia and New Zealand as well as an insight into the association of HBV genetic hypervariability and prevalence of well reported mutations.

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Section: Discussioncontrasting

confidence: 99%

“…This is likely due to the geographical selection of HBV sequences used in this study. We targeted Southeast Asia, Australia and New Zealand, while the Norder study [13] was worldwide and the Chen study [71] focused on China.…”

Section: Discussionmentioning

confidence: 99%

In Silico Analysis of Genetic Diversity of Human Hepatitis B Virus in Southeast Asia, Australia and New Zealand

Phan

Faddy

Flower

et al. 2020

Viruses

View full text Add to dashboard Cite

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“…Similar to the HBV genotype profile identified in the overt HBV infection in Northwest China, the dominant genotype isolated from OBI cases was genotype D (77.5 %, 62/80), followed by genotype C (21.25%, 17/80) and genotype B (1.25%, 1/80). These results suggest that the genotype composition of OBI was the same as the overt HBV infection in this region.…”

Section: Discussionsupporting

confidence: 61%

Prevalence and natural course of occult hepatitis B virus infection in residents of 2 communities of Wuwei City, Gansu Province, China

Wang

et al. 2017

Journal of Viral Hepatitis

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Occult hepatitis B infection (OBI) is characterized by serum hepatitis B surface antigen (HBsAg) negative and hepatitis B virus (HBV) DNA positive (HBsAg-/HBV DNA+). Occult hepatitis B infection in community-based populations has been scarcely investigated, and OBI outcomes remain unclear, especially in Wuwei, a region located in Northwest China. This region is one of the areas in China that has the highest prevalence of chronic HBV infection. A prospective study was performed in the general population of 2 towns of Wuwei from June 2011 to May 2014. A questionnaire was used to collect demographic and medical data, and serum samples were collected from the participants and stored until analysis. DNA was detected using quantitative PCR (qPCR) or nested PCR, the HBV DNA from HBV DNA-positive or possible positive (below the detection limit) subjects was extracted and amplified by nested PCR, and the PCR products were sequenced. Sequence analysis was performed using the Mega 6.0 program and CLC sequence viewer software. Hepatitis B virus DNA was detected in 90 of 3,080 HBsAg-negative subjects, and the prevalence of OBI in the study population was 2.92% (90/3,080, 95% CI: 2.33%-3.51%). Hepatitis B virus genomes in 51 of 80 objects (63.75%) contained mutations in the "a" determinant of HBsAg. After 2 years follow-up, 42 of 90 HBV DNA of OBI subjects remained positive, and the natural clearance rate of OBI subjects was 53.3%. Occult hepatitis B infection prevalence in this cohort was much lower than chronic HBV infection in the same region. HBV DNA was cleared in most OBI subjects during the 2 year period. Our data suggest that some OBI may represent a late stage of resolving the HBV infection process.

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“…Moreover, our results highlight that identification of the genotype-dependent sites would be important for the distinction between mutations and mere polymorphisms during mutation analysis, for example, glutamine at rt238 as a low-frequency AA residue in our study was more likely to be a naturally occurring mutation rather than a polymorphism site. In addition, the standard for differentiating genotypes by our genotype-dependent sites analysis may [23,24]. How HBV genotypes potentially modulate NAs resistant mutation still remains controversial [14,25,26].…”

Section: Discussionmentioning

confidence: 99%

Mutational characterization of HBV reverse transcriptase gene and the genotype-phenotype correlation of antiviral resistance among Chinese chronic hepatitis B patients

et al. 2020

Emerging Microbes & Infections

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Background and Aims: The drug resistance of hepatitis B virus (HBV) originates from mutations within HBV reverse transcriptase (RT) region during the prolonged antiviral therapy. So far, the characteristics of how these mutations distribute and evolve in the process of therapy have not been clarified yet. Thus we aimed to investigate these characteristics and discuss their contributing factors. Methods: HBV RT region was direct-sequenced in 285 treatment-naive and 214 post-treatment patients. Mutational frequency and Shannon entropy were calculated to identify the specific mutations differing between genotypes or treatment status. A typical putative resistance mutation rtL229V was further studied using in-vitro susceptibility assays and molecular modeling. Results: The classical resistance mutations were rarely detected among treatment-naive individuals, while the putative resistance mutations were observed at 8 AA sites. rtV191I and rtA181T/V were the only resistance mutations identified as genotype-specific mutation. Selective pressure of drug usage not only contributed to the classical resistance mutations, but also induced the changes at a putative resistance mutation site rt229. rtL229V was the major substitution at the site of rt229. It contributed to the most potent suppression of viral replication and reduced the in-vitro drug susceptibility to entecavir (ETV) when coexisting with rtM204V, consistent with the hypothesis based on the molecular modeling and clinical data analysis. Conclusions: The analysis of mutations in RT region under the different circumstances of genotypes and therapy status might pave the way for a better understanding of resistance evolution, thus providing the basis for a rational administration of antiviral therapy.

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A description of the hepatitis B virus genomic background in a high-prevalence area in China

Cited by 9 publications

References 19 publications

In Silico Analysis of Genetic Diversity of Human Hepatitis B Virus in Southeast Asia, Australia and New Zealand

In Silico Analysis of Genetic Diversity of Human Hepatitis B Virus in Southeast Asia, Australia and New Zealand

Prevalence and natural course of occult hepatitis B virus infection in residents of 2 communities of Wuwei City, Gansu Province, China

Mutational characterization of HBV reverse transcriptase gene and the genotype-phenotype correlation of antiviral resistance among Chinese chronic hepatitis B patients

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