A Design of Microfluidic Chip with Quasi‐Bessel Beam Waveguide for Scattering Detection of Label‐Free Cancer Cells

Abstract

Light scattering detection in microfluidic chips provides an important tool to identify cancer cells without any label processes. However, forward small‐angle scattering signals of cells, which are related to their sizes and morphologies, are hard to be detected accurately when scattering angle is less than 11° in microfluidic chips by traditional lighting design due to the influence of incident beam. Therefore, cell's size and morphology being the golden standard for clinical detection may lose their efficacy in recognizing cancer cells from healthy ones. In this article, a novel lighting design in microfluidic chips is put forward in which traditional incident Gaussian beam can be modulated into quasi‐Bessel beam by a microprism and waveguide. The quasi‐Bessel beam's advantages of nondiffraction theoretically make forward scattering (FS) detection less than 11° possibly. Our experimental results for peripheral blood lymphocytes of human beings and cultured HeLa cells show that the detection rates increase by 47.87% and 46.79%, respectively, by the novel designed microfluidic chip compared to traditional Gaussian lighting method in microfluidic chips. © 2019 International Society for Advancement of Cytometry

Microfluidics for the rapid detection of Staphylococcus aureus using antibody-coated microspheres

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“…Along with the continuous development of science and technology, as a result, new rapid detection methods have been developed in attempts to search for methods that suitable for on-site detection of bacteria in many fields [ 16 , 18 ]. In recent years, microfluidic chip is a powerful tool technology with the characteristics of integration, miniaturization, automatic sampling, and high-throughput, which highlights the advantages of bacterial detection.…”

“…In recent years, microfluidic chip is a powerful tool technology with the characteristics of integration, miniaturization, automatic sampling, and high-throughput, which highlights the advantages of bacterial detection. Microfluidic technologies have been applied in many fields such as clinical blood diagnosis, immunology, and cancer biology [ 18 , 22 , 23 ]. However, microfluidics efficacy has not been fully demonstrated [ 19 ], such as the limit of optical detections based microfluidic systems detection is only around pg/mL [ 24 ], other methods can detect very low concentration pathogens but suffer from expensive detection and difficult to operate the chip-based assay [ 17 , 25 ].…”

On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale

“…The proposed device involved modulation of the incident Gaussian beam into a quasi-Bessel beam using microprisms and waveguides. The non-diffracting characteristics of the quasi-Bessel beam demonstrated significant improvement (around 50%) in detection accuracy compared to traditional Gaussian lighting methods in microfluidics [ 180 ]. Watts et al, introduced a new chip design that not only captures forward scattered light (0.5–5°), enabling beam shaping and utilizing a cost-efficient light source with low quality and high divergence, but also efficiently blocks transmitted light.…”

Developing a Reliable Holographic Flow Cyto-Tomography Apparatus by Optimizing the Experimental Layout and Computational Processing

“…Moreover, the invasive and destructive nature of staining processes constitutes a significant motivation for researchers to develop label-free tools. Innovative no-contact and marker-free strategies have been modeled and tested in microfluidics and optics [ 2 , 3 , 4 , 5 , 6 ], and are set to become the key technologies in the biomedical field. Among them, Quantitative Phase Imaging (QPI) is a promising technique because the retrieved images quantitatively measure the optical thickness [ 7 , 8 ].…”