Microfluidics for the rapid detection of
            <i>Staphylococcus aureus</i>
            using antibody-coated microspheres

Shopsin, Bo; Wang, Junsheng; Yan, Zhijun; Liu, Zhijian; Pan, Xinxiang; Zhang, Yingbo; Zhang, Xiaojie

doi:10.1080/21655979.2020.1831362

Cited by 19 publications

(7 citation statements)

References 34 publications

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“…The Ng strain was reconstituted from frozen stock cultures and propagated at 37°C with 5% CO 2 on GC agar (OXOID, Basingstoke, UK). Before each treatment, the bacterial concentration was adjusted to 5 × 10 6 CFU mL −1 (colony-forming units per milliliter)[ 14 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae

et al. 2021

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Neisseria gonorrhoeae (Ng) is highly resistant to treatment, and there is an urgent need for new treatments to alleviate gonococcal resistance caused by antibiotic monotherapy. The antimicrobial effect and mechanism of plasma-activated liquid (PAL) on Ng were evaluated in this study. Upon PAL treatment, extensively analyses on cell culturability, metabolic capacity, intracellular reactive oxygen species (ROS),membrane integrity and nucleic acids for Ng were carried out and significant antimicrobial effects observed.PAL exerted antibacterial effect on Ng and induced bacterial death (6.71-log) following immersion for 30 min and treatment for 120 s. However, bacterial viability test revealed that after immersion in the same PAL, 10.17% of bacteria retained their metabolic capacity. This indicates that bacteria enter a physiological viable but nonculturable state to protect themselves from environmental stress. Confocal fluorescence microscopy and transmission electron microscopy demonstrated that PAL exerts bactericidal effect on Ng and disrupts its morphological structure. PAL may upregulate inflammatory factors and genes to modulate the resistance of Ng and affect the immune status of the host during infection.

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Section: Methodsmentioning

confidence: 99%

In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae

et al. 2021

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“…For 2 μL/min flow rate, the fluorescence intensity reaches its maximum functionality, while a 5 μL/min flow rate and a 4 min duration are the optimal conditions for the optimal injection and reaction. At this flow rate, the target bacteria can be seen from the microsphere’s surface [ 209 ].…”

Section: Microfluidics and Locs In Food Safety Applicationsmentioning

confidence: 99%

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

2023

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Food quality and safety are important to protect consumers from foodborne illnesses. Currently, laboratory scale analysis, which takes several days to complete, is the main way to ensure the absence of pathogenic microorganisms in a wide range of food products. However, new methods such as PCR, ELISA, or even accelerated plate culture tests have been proposed for the rapid detection of pathogens. Lab-on-chip (LOC) devices and microfluidics are miniaturized devices that can enable faster, easier, and at the point of interest analysis. Nowadays, methods such as PCR are often coupled with microfluidics, providing new LOC devices that can replace or complement the standard methods by offering highly sensitive, fast, and on-site analysis. This review’s objective is to present an overview of recent advances in LOCs used for the identification of the most prevalent foodborne and waterborne pathogens that put consumer health at risk. In particular, the paper is organized as follows: first, we discuss the main fabrication methods of microfluidics as well as the most popular materials used, and then we present recent literature examples for LOCs used for the detection of pathogenic bacteria found in water and other food samples. In the final section, we summarize our findings and also provide our point of view on the challenges and opportunities in the field.

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“…in the sample measured by the microchip was approximately 12 CFU/ml. Song et al (2020) developed a microfluidic platform to detect S. aureus by fluorescence labeling method and a self-made microfluidic chip, which has immune spheres were used to study the effect of capturing S. aureus ( Figure 4C ). Results showed that our platform can detect S. aureus at an injection rate of 5 μl/min reacted for 4 min and the detection limit of bacteria was 1.5 × 10 1 CFU/mul.…”

Section: Microfluidic Detection Technologymentioning

confidence: 99%

“… (A) Schematic of this Colorimetric Biosensor for Detection of Salmonella ( Xue et al, 2021 ); (B) Making the process of the microchip ( Zhang et al, 2021b ); (C) The whole process of a microfluidic platform for detection of S. aureus by fluorescence labeling ( Song et al, 2020 ); (D) Schematics of the i-Gene ( Nguyen et al, 2020 ). …”

Section: Microfluidic Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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Microfluidics for the rapid detection of Staphylococcus aureus using antibody-coated microspheres

Cited by 19 publications

References 34 publications

In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae

In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic Neisseria gonorrhoeae

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

Research progress on detection techniques for point-of-care testing of foodborne pathogens

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