Research progress on detection techniques for point-of-care testing of foodborne pathogens

Abstract

The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

Molecular Targets for Foodborne Pathogenic Bacteria Detection

“…However, such an approach increased the cost of detection and required trained personnel [ 176 ]. Attempts to concentrate the target cells (e.g., through immunological techniques) have not improved the sensitivity and specificity of the detection and, therefore, have not met wide acceptance [ 177 ].…”

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

“…State-of-the-art microfluidic devices for nucleic acid tests (NATs) were summarized in the literature. [6][7][8][9][10][11][12] Some notable examples of microfluidic NAT systems were centrifugal microdevices, paper-based microdevices, channel-based continuous flow chips, and digital microfluidic (DMF) devices. Paper-based microdevices, such as lateral flow assay strips, have been widely employed for point-of-care testing (POCT) due to the characteristics of low-cost, single configuration, and ease of operation.…”

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria

“…14 not relying on sophisticated instruments such as a spectrometer 15 or a smartphone-based microscope. 14 For example, three-dimensional microfluidic paper-based analytical devices (3D-μPADs) offer an affordable solution for analyzing multiplexed assays. 16 However, one major challenge with μPADs and all different kinds of microfluidics is distribution homogeneity, where channels must have the same hydrodynamic resistance for every branch in a certain region to prevent a bias in the final output.…”