A determination method of pristine multiwall carbon nanotubes in rat lungs after intratracheal instillation exposure by combustive oxidation–nondispersive infrared analysis

Tamura, Moritaka; Inada, Masaharu; Nakazato, Tetsuya; Yamamoto, Kazuhiro; Endo, Shigehisa; Uchida, Kunio; Horie, Masanori; Fukui, Hitoshi; Iwahashi, Hitoshi; Kobayashi, Norihiro; Morimoto, Yasuo; Tao, Hiroaki

doi:10.1016/j.talanta.2011.02.017

Cited by 11 publications

(15 citation statements)

References 26 publications

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“…The precision of analysis (repeatability) for pulmonary MWCNTs determination was 5.6% and the efficiency of recovery was > 95% (Tamura et al ., 2011). …”

Section: Resultsmentioning

confidence: 99%

“…The detection limit and the precision of analysis for pulmonary MWCNTs in the present study were checked in the previous study (Tamura et al ., 2011). To check the validity of this method, the lung burdens of male Wistar rats (aged 8 weeks, mean body weight 272 g, range 245–299 g) were determined after a 28‐day inhalation whole‐body MWCNTs exposure (0.37 ± 0.18 mg m −3 ), and compared with our previously reported results for the same inhalation exposure using different methods (Oyabu et al ., 2011).…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, we administered the MWCNTs intratracheally to rats and determined lung, liver, and brain MWCNTs levels for 12 months. To achieve this, we used a recently developed method (Tamura et al ., 2011) involving non‐dispersive infrared (NDIR) analysis of the CO 2 generated from the decomposition of pristine MWCNTs that was separated from tissues by acid‐digestion and heat pretreatment. Pulmonary pristine MWCNTs were also observed over time using optical microscopy and transmission electron microscopy (TEM).…”

Section: Introductionmentioning

confidence: 99%

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Long‐term retention of pristine multi‐walled carbon nanotubes in rat lungs after intratracheal instillation

Shinohara

Nakazato

Ohkawa

et al. 2015

J of Applied Toxicology

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As a result of the growing potential industrial and medical applications of multi‐walled carbon nanotubes (MWCNTs), people working in or residing near facilities that manufacture them may be exposed to airborne MWCNTs in the future. Because of concerns regarding their toxicity, quantitative data on the long‐term clearance of pristine MWCNTs from the lungs are required. We administered pristine MWCNTs well dispersed in 0.5 mg ml−1 Triton‐X solution to rats at doses of 0.20 or 0.55 mg via intratracheal instillation and investigated clearance over a 12‐month observation period. The pristine MWCNTs pulmonary burden was determined 1, 3, 7, 28, 91, 175 and 364 days after instillation using a method involving combustive oxidation and infrared analysis, combined with acid digestion and heat pretreatment. As 0.15‐ and 0.38‐mg MWCNTs were detected 1 day after administration of 0.20 and 0.55 mg MWCNTs, respectively, approximately 30% of administrated MWCNTs may have been cleared by bronchial ciliary motion within 24 h of administration. After that, the pulmonary MWCNT burden did not decrease significantly over time for up to 364 days after instillation, suggesting that MWCNTs were not readily cleared from the lung. Transmission electron microscopy (TEM) showed that alveolar macrophages internalized the MWCNTs and retained in the lung for at least 364 days after instillation. MWCNTs were not detected in the liver or brain within the 364‐day study period (<0.04 mg per liver, < 0.006 mg per brain). Copyright © 2015 The Authors Journal of Applied Toxicology Published by John Wiley & Sons Ltd.

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“…The precision of analysis (repeatability) for pulmonary MWCNTs determination was 5.6% and the efficiency of recovery was > 95% (Tamura et al ., 2011). …”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Long‐term retention of pristine multi‐walled carbon nanotubes in rat lungs after intratracheal instillation

Shinohara

Nakazato

Ohkawa

et al. 2015

J of Applied Toxicology

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“…Before injection of the SWNTs solution, a Raman spectrum was recorded for calculating the SWNTs concentration based on the calibration curve (Tamura et al 2011).…”

Section: Reagentsmentioning

confidence: 99%

Sodium Carboxyl Methyl Cellulose for Improvement of the Determination of Polyethylene Glycol Modified Carbon Nanotubes by Raman Spectroscopy

Han

Zhang²,

Zheng³

et al. 2013

Analytical Letters

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Single-walled carbon nanotubes (SWNTs) have shown great potential in various areas of biomedicine. However, few research studies have focused on in vivo carbon nanotube detection by Raman spectroscopy. In this work, SWNTs were functionalized with polyethylene glycol (PEG) to form PEG-o-SWNTs to improve the biocompatibility and dispersibility. A novel application of Raman scattering was used to detect the PEG-o-SWNTs. Compared to the traditional assay method, the recovery rate was improved from 84.2% to 102.7%, the correlation coefficient of the standard curve increased from 0.7315 to 0.9872, and the degree of precision decreased from 4.4% to 3.1%. Large errors in the measured results were found the cryopreserved blood and organ samples in which the reticular sediments separated, on which the nanotubes generally concentrate. It was determined that by achieving a final concentration of 0.5% through the addition of sodium carboxyl methyl cellulose (CMC-Na), the homogeneity of the tested sample can be greatly enhanced, thus boosting the accuracy of the analyses. This work offers methods with higher accuracy for determining the presence of PEG-o-SWNTs in blood and organs using Raman spectroscopy.

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“…In the past decade, multi-walled carbon nanotube (MWCNT) polymer composites have attracted strong interest in academia and various industries, such as battery fabrication, 1 optoelectronics 2 and biological tagging, 3 because of their peculiar electrical performance, good°exibility, outstanding chemical stability, heat stability and adsorption characteristics. 4 In addition, MWCNTs have high speci¯c ratios but low density 5 which make them excellent additives to improve the mechanical, 6 tribological 7-9 and rheological properties, 10 decrease the percolation thresholds 11 of the matrix.…”

Section: Introductionmentioning

confidence: 99%

Tribology Property of Benzoxazine–Bismaleimide Composites with Hyperbranched Polysilane-Grafted Multi-Walled Carbon Nanotubes

Jia

Yan

et al. 2016

NANO

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To reveal the wear mechanism of hyperbranched polysilane (HBPSi) grafted multi-walled carbon nanotubes (HBPSi-MWCNTs) modi¯ed benzoxazine-bismaleimide (BOZ-BMI) resin (HBPSiMWCNTs/BOZ-BMI), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA) were employed. The results indicated that the suitable addition of HBPSi-MWCNTs could largely enhance the tribological properties of BOZ-BMI composites. The worn surface of the composites showed that the severe wear of the BOZ-BMI resin was converted from adhesive wear to abrasive wear with the addition of HBPSiMWCNTs. The excellent tribological properties can be attributed to the improved interfacial adhesion between HBPSi-MWCNTs and the BOZ-BMI resin matrix. The TGA demonstrated that the composite with 0.8 wt.% HBPSi-MWCNTs exhibits better thermal resistance; thus, it can inhibit adhesive wear during the friction process. The XPS spectra and the surface energy showed that the HBPSi-MWCNTs could be exposed on the worn surface of the composite to improve the anti-wear capacity of the composites further.

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A determination method of pristine multiwall carbon nanotubes in rat lungs after intratracheal instillation exposure by combustive oxidation–nondispersive infrared analysis

Cited by 11 publications

References 26 publications

Long‐term retention of pristine multi‐walled carbon nanotubes in rat lungs after intratracheal instillation

Long‐term retention of pristine multi‐walled carbon nanotubes in rat lungs after intratracheal instillation

Sodium Carboxyl Methyl Cellulose for Improvement of the Determination of Polyethylene Glycol Modified Carbon Nanotubes by Raman Spectroscopy

Tribology Property of Benzoxazine–Bismaleimide Composites with Hyperbranched Polysilane-Grafted Multi-Walled Carbon Nanotubes

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