A Development-Associated Decrease in Osmotic Potential Contributes to Fruit Ripening Initiation in Strawberry (Fragaria ananassa)

Jia, Kenan; Zhang, Qing; Xing, Yu; Yan, Jiaqi; Liu, Luo; Nie, Kaili

doi:10.3389/fpls.2020.01035

Cited by 14 publications

(6 citation statements)

References 67 publications

(100 reference statements)

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“…However, little attention has been paid in the literature to the significance of the osmotic potential-associated changes in the regulation of fruit ripening. Such an effect was recently shown in a study by Jia et al [ 17 ] where a sharp decline in cellular osmotic potential coincided with the onset of strawberry fruit ripening. Moreover, by manipulating the changes in osmotic potential, the authors reported being able to modulate ripening.…”

Section: Introductionsupporting

confidence: 56%

“…Given that fruit ripening is such an important part of the life cycle of many plants, it is not surprising that many studies have shown that the progression of fruit ripening is sensitive to environmental signals. For example, the progression of strawberry fruit ripening is extremely sensitive to illumination, temperature and water availability [ 17 , 83 ]. A minor elevation in temperature, (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Signal transduction in non-climacteric fruit ripening

Wang

Fan

Hao

et al. 2022

Horticulture Research

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Fleshy fruit ripening involves changes in numerous cellular processes and metabolic pathways, resulting from the coordinated actions of diverse classes of structural and regulatory proteins. These include enzymes, transporters and complex signal transduction systems. Many aspects of the signaling machinery that orchestrates the ripening of climacteric fruits, such as tomato (Solanum lycopersicum), have been elucidated, but less is known about analogous processes in non-climacteric fruits. The latter include strawberry (Fragaria x ananassa) and grape (Vitis vinifera), both of which are used as non-climacteric fruit experimental model systems, although they originate from different organs: the grape berry is a true fruit derived from the ovary, while strawberry is an accessory fruit that is derived from the floral receptacle. In this article, we summarize insights into the signal transduction events involved in strawberry and grape berry ripening. We highlight the mechanisms underlying non-climacteric fruit ripening, the multiple primary signals and their integrated action, individual signaling components, pathways and their crosstalk, as well as the associated transcription factors and their signaling output.

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Section: Introductionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Signal transduction in non-climacteric fruit ripening

Wang

Fan

Hao

et al. 2022

Horticulture Research

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“…Cold stress is usually accompanied by dehydration and osmotic imbalance with time extending. Studies have certified that osmotin had a function in osmoregulation under stress and had adapted to low osmotic potential environments [ 14 , 24 , 56 ]. The expression levels of KoOsmotin were increased to substantial accumulation after 15 d under cold stress in among leaves, stems and roots.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress

et al. 2021

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Background Low temperature is a major abiotic stress that seriously limits mangrove productivity and distribution. Kandelia obovata is the most cold-resistance specie in mangrove plants, but little is known about the molecular mechanism underlying its resistance to cold. Osmotin is a key protein associated with abiotic and biotic stress response in plants but no information about this gene in K. obovata was reported. Results In this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal signal peptide, which were common signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) model of KoOsmotin, contained one α-helix and eleven β-strands, was formed by three characteristic domains. Database comparisons of the KoOsmotin showed the closest identity (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree also revealed that the KoOsmotin was clustered in the branch of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to both the plasma membrane and cytoplasm by the subcellular localization analysis. Gene expression showed that the KoOsmotin was induced primarily and highly in the leaves of K. obovata, but less abundantly in stems and roots. The overexpressing of KoOsmotin conferred cold tolerance in Escherichia coli cells. Conclusion As we known, this is the first study to explore the osmotin of K. obovata. Our study provided valuable clues for further exploring the function of KoOsmotin response to stress.

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“…N. benthamiana plants were used at 5.5 weeks old and the two youngest leaves chosen for at least six infiltration patches. Mature strawberry fruit (with fully developed seeds) were infiltrated at the initial reddening stage as described by (Jia et al, 2020) (Supplementary Figure 1A).…”

Section: Plant Materialsmentioning

confidence: 99%

Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production

et al. 2023

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Anthocyanins are a major group of red to blue spectrum plant pigments with many consumer health benefits. Anthocyanins are derived from the flavonoid pathway and diversified by glycosylation and methylation, involving the concerted action of specific enzymes. Blueberry and bilberry (Vaccinium spp.) are regarded as ‘superfruits’ owing to their high content of flavonoids, especially anthocyanins. While ripening-related anthocyanin production in bilberry (V. myrtillus) and blueberry (V. corymbosum) is regulated by the transcriptional activator MYBA1, the role of specific structural genes in determining the concentration and composition of anthocyanins has not been functionally elucidated. We isolated three candidate genes, CHALCONE SYNTHASE (VmCHS1), ANTHOCYANIDIN SYNTHASE (VmANS) and UDP-GLUCOSE : FLAVONOID-3-O-GLYCOSYLTRANSFERASE (VcUFGT2), from Vaccinium, which were predominantly expressed in pigmented fruit skin tissue and showed high homology between bilberry and blueberry. Agrobacterium-mediated transient expression of Nicotiana benthamiana showed that overexpression of VcMYBA1 in combination with VmANS significantly increased anthocyanin concentration (3-fold). Overexpression of VmCHS1 showed no effect above that induced by VcMYBA1, while VcUFGT2 modulated anthocyanin composition to produce delphinidin-3-galactosylrhamnoside, not naturally produced in tobacco. In strawberry (Fragaria × ananassa), combined transient overexpression of VcUFGT2 with a FLAVONOID 3´,5´-HYDROXYLASE from kiwifruit (Actinidia melanandra) modulated the anthocyanin profile to include galactosides and arabinosides of delphinidin and cyanidin, major anthocyanins in blueberry and bilberry. These findings provide insight into the role of the final steps of biosynthesis in modulating anthocyanin production in Vaccinium and may contribute to the targeted breeding of new cultivars with improved nutritional properties.

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A Development-Associated Decrease in Osmotic Potential Contributes to Fruit Ripening Initiation in Strawberry (Fragaria ananassa)

Cited by 14 publications

References 67 publications

Signal transduction in non-climacteric fruit ripening

Signal transduction in non-climacteric fruit ripening

Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress

Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production

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