A Device for Dried Blood Microsampling in Quantitative Bioanalysis: Overcoming The Issues Associated Blood Hematocrit

Abstract

The novel volumetric absorptive microsampling device has the potential to deliver the advantages of dried blood spot sampling while overcoming some of the issues associated with the technology.

Hematocrit-Independent Recovery is A Key for Bioanalysis Using Volumetric Absorptive Microsampling Devices, Mitra Tm

“…The Hct effect in DBS is mainly due to a constant punch size in spite of variable size of blood spot depending on the Hct, however, impacts of Hct on DBS might be partly overcome by optimization in extraction procedure as observed in the present study. Mitra has overcome the Hct-dependent variable volume of blood subjected to the assay as demonstrated by Spooner et al [3]. The present study has successfully demonstrated that optimization in extraction process to give high and consistent extraction recovery is of importance for accurate assay using Mitra.…”

“…In addition, some abuses during sampling and handling were also evaluated such as repeat dipping of the tip in samples, effects of submerging of the tip and postsampling abuses including tip touched against paper towel. In addition, the latest paper by Spooner et al [3] has demonstrated that the device absorbed a fixed volume of whole blood (~10 μl) regardless of Hct values across six different laboratories, suggesting minimal interlaboratory differences in sampling volume via Mitra. However, to the best of our knowledge, impacts of Hct on the accuracy of analyses have not been reported except in one recently pub-lished paper [4].…”

Analysis of whole blood by ICP-MS equipped with a high temperature total sample consumption system

“…46,47 Furthermore, the collected sample volume is independent of the hematocrit level. 48 It has been very recently demonstrated that, when aiming at multi-elemental analysis, this methodology allows the recovery of the sample in a simple way by applying a single extraction step with 1% HNO 3 without any further manipulation.…”

Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples

“…Thus, where estimation of HCT is made, there may be a need to enforce strict acceptance criteria for quality of received DBS specimens, such as a minimum apparent volume [5]. Last, it is worth noting that advanced collection techniques involving volumetric application of blood to filter paper for whole-spot analysis could obviate any need to estimate sample hematocrit from DBS [14,15].…”